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Start Over Author "Lingxiang Zhu" Journal thoracic cancer
2 results on '"Lingxiang Zhu"'

1. Clinical evaluation of the effectiveness of fusion‐induced asymmetric transcription assay‐based reverse transcription droplet digital PCR for ALK detection in formalin‐fixed paraffin‐embedded samples from lung cancer

Author

Yuanyuan Liu, Shafei Wu, Xiaohua Shi, Linping Lu, Lingxiang Zhu, Yong Guo, Li Zhang, and Xuan Zeng

Subjects
ALK, asymmetric, droplet digital PCR, lung cancer, rearrangement, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282
Abstract

Background Accurate detection of anaplastic lymphoma kinase (ALK) rearrangement is the prerequisite for anti‐ALK therapy for the patient with non‐small cell lung cancer (NSCLC). Fusion‐induced asymmetric transcription assay (FIATA)‐based reverse transcription droplet digital PCR (RT‐ddPCR) was developed and performed for ALK status survey in NSCLC samples. Methods A total of 269 cases of formalin‐fixed paraffin‐embedded (FFPE) specimens from NSCLC, in which ALK status was confirmed by both fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC), were analyzed by FIATA‐based RT‐ddPCR. Results In the ALK‐positive group, the 3′ ALK transcript copies range was 336.6–107 955.4, and the R3 [(the ratio of the 3′ ALK transcript copy numbers to the internal reference gene transcript copy numbers) × 100] was 17.23–672.77. In the ALK‐negative group, the 3′ ALK transcript copies range was 3.7–1370.6, and the R3 range was 0.10–15.57. The lowest R3 level in the ALK‐positive group was significantly higher than the highest R3 level in the ALK‐negative group. A positive correlation between the proportion of cancer cells in the tissue section and ALK RNA expression level (R3) was found (P

Published
2020
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2. Clinical evaluation of the effectiveness of fusion‐induced asymmetric transcription assay‐based reverse transcription droplet digital <scp>PCR</scp> for <scp> ALK </scp> detection in formalin‐fixed paraffin‐embedded samples from lung cancer

Author

Shafei Wu, Yuanyuan Liu, Guo Yong, Lingxiang Zhu, Lu Linping, Li Zhang, Xiaohua Shi, and Xuan Zeng

Subjects
0301 basic medicine, Pulmonary and Respiratory Medicine, medicine.diagnostic_test, business.industry, General Medicine, medicine.disease, Molecular biology, Reverse transcriptase, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, Oncology, Transcription (biology), hemic and lymphatic diseases, 030220 oncology & carcinogenesis, Cancer cell, medicine, Immunohistochemistry, Anaplastic lymphoma kinase, Digital polymerase chain reaction, Lung cancer, business, Fluorescence in situ hybridization
Abstract

BACKGROUND Accurate detection of anaplastic lymphoma kinase (ALK) rearrangement is the prerequisite for anti-ALK therapy for the patient with non-small cell lung cancer (NSCLC). Fusion-induced asymmetric transcription assay (FIATA)-based reverse transcription droplet digital PCR (RT-ddPCR) was developed and performed for ALK status survey in NSCLC samples. METHODS A total of 269 cases of formalin-fixed paraffin-embedded (FFPE) specimens from NSCLC, in which ALK status was confirmed by both fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC), were analyzed by FIATA-based RT-ddPCR. RESULTS In the ALK-positive group, the 3' ALK transcript copies range was 336.6-107 955.4, and the R3 [(the ratio of the 3' ALK transcript copy numbers to the internal reference gene transcript copy numbers) × 100] was 17.23-672.77. In the ALK-negative group, the 3' ALK transcript copies range was 3.7-1370.6, and the R3 range was 0.10-15.57. The lowest R3 level in the ALK-positive group was significantly higher than the highest R3 level in the ALK-negative group. A positive correlation between the proportion of cancer cells in the tissue section and ALK RNA expression level (R3) was found (P

Published
2020

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