1. Gut microbiome affects the response to immunotherapy in non‐small cell lung cancer.
- Author
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Ren, Shengnan, Feng, Lingxin, Liu, Haoran, Mao, Yuke, and Yu, Zhuang
- Subjects
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FECAL analysis , *FECAL microbiota transplantation , *SHORT-chain fatty acids , *ACETIC acid , *STATISTICAL significance , *RESEARCH funding , *IMMUNOTHERAPY , *GUT microbiome , *PROGRAMMED death-ligand 1 , *KRUSKAL-Wallis Test , *MULTIPLE regression analysis , *TUMOR markers , *CHI-squared test , *MANN Whitney U Test , *MULTIVARIATE analysis , *DESCRIPTIVE statistics , *IMMUNE checkpoint inhibitors , *MICE , *RNA , *GENE expression , *ANIMAL experimentation , *ANALYSIS of variance , *LUNG cancer , *BUTYRIC acid , *CONFIDENCE intervals , *DATA analysis software , *BIOMARKERS , *SEQUENCE analysis , *ACYCLIC acids , *DISCRIMINANT analysis , *PROPORTIONAL hazards models - Abstract
Background: Immunotherapy has revolutionized cancer treatment. Recent studies have suggested that the efficacy of immunotherapy can be further enhanced by the influence of gut microbiota. In this study, we aimed to investigate the impact of bacteria on the effectiveness of cancer immunotherapy by combining analysis of clinical samples with validation in animal models. Methods: In order to characterize the diversity and composition of microbiota and its relationship with response to immune checkpoint inhibitors (ICIs), 16S ribosomal RNA (rRNA) and GC–MS sequencing was performed on 71 stool samples from patients with advanced non‐small cell lung cancer (NSCLC) prior to treatment with immune checkpoint blockade (ICB). Furthermore, fecal microbiota transplantation (FMT) was performed from different patients into mice and a subcutaneous tumor model established using the Lewis lung cancer cell line to evaluate the therapeutic effect of PD‐1 on mice with varying gut microbiota. Results: The results demonstrated a significant association between elevated gut microbiota diversity and response to treatment with ICIs, p < 0.05. Faecalibacterium was markedly increased in the gut microbiota of responders (R), accompanied by increased short‐chain fatty acid (SCFA) levels, especially butanoic acid, acetic acid and hexanoic acid, p < 0.05. Additionally, FMT from R and nonresponders (NR) could promote an anticancer effect and reduce the expression of Ki‐67 cells in tumors in mice, p < 0.05. Moreover, R and NR FMT did not alter PD‐L1 expression in the tumor tissues of mice, p > 0.05. The diversity of gut microbiota consistently correlated with an optimistic prognosis in NSCLC patients with immunotherapy, which could be functionally mediated by SCFAs. Conclusion: The findings of the present study indicated that the diversity of gut microbiota and SCFAs is related to the efficacy of immunotherapy. FMT can effectively delay tumor progression, and enhance the effect of immunotherapy, thus providing evidence for improving the efficacy of immunotherapy in NSCLC patients. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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