7 results on '"Zonghai Sheng"'
Search Results
2. In vivo assessment of inflammation in carotid atherosclerosis by noninvasive photoacoustic imaging
- Author
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Shaohong Dong, Huadong Liu, Jingqin Chen, Yaqiong He, Yanqing Yang, Xiaojing Gong, Chengyou Shu, Zonghai Sheng, Chengbo Liu, Liang Song, Dong Chen, Jie Liu, Zhihua Xie, and Jinke Zhang
- Subjects
Carotid atherosclerosis ,Noninvasive imaging ,Carotid arteries ,carotid atherosclerosis ,Medicine (miscellaneous) ,Photoacoustic imaging in biomedicine ,Inflammation ,02 engineering and technology ,030204 cardiovascular system & hematology ,03 medical and health sciences ,0302 clinical medicine ,In vivo ,noninvasive photoacoustic imaging ,medicine ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,business.industry ,PBD-CD36 ,021001 nanoscience & nanotechnology ,molecular imaging ,inflammation ,Molecular imaging ,medicine.symptom ,0210 nano-technology ,business ,Preclinical imaging ,Biomedical engineering ,Research Paper - Abstract
Objectives: The objective of this study was to demonstrate the feasibility of using noninvasive photoacoustic imaging technology along with novel semiconducting polymer nanoparticles for in vivo identifying inflammatory components in carotid atherosclerosis and assessing the severity of inflammation using mouse models. Methods and Results: Healthy carotid arteries and atherosclerotic carotid arteries were imaged in vivo by the noninvasive photoacoustic imaging system. Molecular probes PBD-CD36 were used to label the inflammatory cells to show the inflammation information by photoacoustic imaging. In in vivo imaging experiments, we observed the maximum photoacoustic signal enhancement of 4.3, 5.2, 8 and 16.3 times between 24 h post probe injection and that before probe injection in four carotid arteries belonging to three atherosclerotic mice models. In the corresponding carotid arteries stained with CD36, the ratio of 0.043, 0.061, 0.082 and 0.113 was found between CD36 positive (CD36(+)) expression area and intima-media area (P < 0.05). For the CD36(+) expression less than 0.008 in eight arteries, no photoacoustic signal enhancement was found due to the limited system sensitivity. The photoacoustic signal reflects CD36(+) expression in plaques, which shows the feasibility of using photoacoustic imaging for in vivo assessment of carotid atherosclerosis. Conclusion: This research demonstrates a semiconducting polymer nanoparticle along with photoacoustic technology for noninvasive imaging and assessment of inflammation of carotid atherosclerotic plaques in vivo.
- Published
- 2020
3. Förster Resonance Energy Transfer-Based Dual-Modal Theranostic Nanoprobe for In Situ Visualization of Cancer Photothermal Therapy
- Author
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Hairong Zheng, Liang Song, Zonghai Sheng, Fei Yan, Chengbo Liu, Dehong Hu, Mingting Zhu, Xin Liu, Ming Qian, and Xiaobing Wang
- Subjects
Materials science ,Theranostic Nanomedicine ,Photothermal effect ,Medicine (miscellaneous) ,Nanoprobe ,Cancer ,02 engineering and technology ,Photothermal therapy ,010402 general chemistry ,021001 nanoscience & nanotechnology ,medicine.disease ,01 natural sciences ,0104 chemical sciences ,Förster resonance energy transfer ,Cancer cell ,medicine ,Photoacoustic Techniques ,0210 nano-technology ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,Biomedical engineering - Abstract
The visualization of the treatment process in situ could facilitate to accurately monitor cancer photothermal therapy (PTT), and dramatically decrease the risk of thermal damage to normal cells and tissues, which represents a major challenge for cancer precision therapy. Herein, we prepare theranostic nanoprobes (NPs) for Forster resonance energy transfer (FRET)-based dual-modal imaging-guided cancer PTT, and clear visualization of the therapeutic process. The FRET-based theranostic NPs exhibit high FRET efficiency (88.2%), good colloidal stability, and tumor-targeting ability. Tumor tissue and surrounding blood vessels are visualized clearly by FRET-based NIR fluorescence imaging with a high signal-to-background ratio (14.5) and photoacoustic imaging with an excellent resolution at 24 h post injection of NPs. Under the guidance of dual-modal imaging, the NPs-induced photothermal effect selectively destructs cancer cells, simultaneously decreasing the FRET efficiency and leading to fluorescence and photoacoustic signal changes. The sensitive self-feedback process enables the in situ visualization of therapeutic process and precision guidance of in vivo cancer PTT. A high therapeutic efficacy and minimum side effects are achieved in C6 tumor-bearing nude mice, holding great promise for precision therapy and cancer theranostics.
- Published
- 2018
4. Molecular Engineering of Near-Infrared Light-Responsive BODIPY-Based Nanoparticles with Enhanced Photothermal and Photoacoustic Efficiencies for Cancer Theranostics
- Author
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Yubin Liu, Duyang Gao, Zonghai Sheng, Zhen Yuan, Boyu Zhang, Xuanjun Zhang, and Dehong Hu
- Subjects
Boron Compounds ,Materials science ,photothermal therapy ,Biocompatibility ,Infrared Rays ,Medicine (miscellaneous) ,Nanoparticle ,Mice, Nude ,Nanotechnology ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Fluorescence ,Theranostic Nanomedicine ,Molecular engineering ,Photoacoustic Techniques ,chemistry.chemical_compound ,Cell Line, Tumor ,Neoplasms ,medicine ,Animals ,Humans ,Bovine serum albumin ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,near-infrared absorption ,Mice, Inbred BALB C ,biology ,Cell Death ,theragnostic nanoparticles ,BODIPY molecular engineering ,Cancer ,Hyperthermia, Induced ,Photothermal therapy ,Phototherapy ,021001 nanoscience & nanotechnology ,medicine.disease ,0104 chemical sciences ,chemistry ,Cancer cell ,biology.protein ,Nanoparticles ,photoacoustic imaging ,BODIPY ,0210 nano-technology ,Research Paper - Abstract
Background: Engineering a single organic-molecule-based nanoparticle integrating precise diagnosis and effective therapy is of great significance for cancer treatment and future clinical applications but remains a great challenge. The goal of this study is to explore small organic molecule-based nanoparticles with high photothermal conversion efficiency for photoacoustic imaging-guided therapy. Methods: Heptacyclic B, O-chelated BODIPY structure (namely Boca-BODIPY) with strong near-infrared (NIR) absorption was designed as a theranostic agent through simply molecular engineering, in which heavy atoms and alkyl chains were introduced to promote its application for tumor theranostics. The Boca-BODIPY molecules are further encapsulated in reduced bovine serum albumin (BSA) through self-assembly. Results: The BSA-Boca-BODIPY exhibited excellent biocompatibility, extraordinary stability and high photothermal conversion efficiency up to 58.7%. The nanoparticles could dramatically enhance photoacoustic contrast of the tumor region, and the signal-to-noise ratio was increased about 14 times at 10 h post intravenous injection in 4T1 tumor-bearing mice. In addition, the nanoassemblies can efficiently convert laser energy (808 nm, 0.75 w cm-2, 5min) into hyperthermia for tumor ablation. Under the photoacoustic imaging-guided photothermal therapy (PTT), the 4T1 cancer cells were efficiently killed, no tumor recurrence and PTT-induced toxicity is observed. Conclusions: Molecular engineering is a promising way to design organic-molecule-based nanoparticles for cancer theranostics. Other organic-molecule-based nanoparticles which show great promise for imaging-guided cancer precision therapy can be engineered through this method.
- Published
- 2019
5. Förster Resonance Energy Transfer-Based Dual-Modal Theranostic Nanoprobe for
- Author
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Dehong, Hu, Zonghai, Sheng, Mingting, Zhu, Xiaobing, Wang, Fei, Yan, Chengbo, Liu, Liang, Song, Ming, Qian, Xin, Liu, and Hairong, Zheng
- Subjects
Indocyanine Green ,Mice, Inbred BALB C ,theranostics ,photothermal therapy ,Förster resonance energy transfer ,Mice, Nude ,Hyperthermia, Induced ,Phototherapy ,Fluorescence ,Theranostic Nanomedicine ,Rats ,Photoacoustic Techniques ,Mice ,human serum albumin ,Cell Line, Tumor ,Neoplasms ,Fluorescence Resonance Energy Transfer ,Animals ,Nanoparticles ,photoacoustic imaging ,Research Paper - Abstract
The visualization of the treatment process in situ could facilitate to accurately monitor cancer photothermal therapy (PTT), and dramatically decrease the risk of thermal damage to normal cells and tissues, which represents a major challenge for cancer precision therapy. Herein, we prepare theranostic nanoprobes (NPs) for Förster resonance energy transfer (FRET)-based dual-modal imaging-guided cancer PTT, and clear visualization of the therapeutic process. The FRET-based theranostic NPs exhibit high FRET efficiency (88.2%), good colloidal stability, and tumor-targeting ability. Tumor tissue and surrounding blood vessels are visualized clearly by FRET-based NIR fluorescence imaging with a high signal-to-background ratio (14.5) and photoacoustic imaging with an excellent resolution at 24 h post injection of NPs. Under the guidance of dual-modal imaging, the NPs-induced photothermal effect selectively destructs cancer cells, simultaneously decreasing the FRET efficiency and leading to fluorescence and photoacoustic signal changes. The sensitive self-feedback process enables the in situ visualization of therapeutic process and precision guidance of in vivo cancer PTT. A high therapeutic efficacy and minimum side effects are achieved in C6 tumor-bearing nude mice, holding great promise for precision therapy and cancer theranostics.
- Published
- 2017
6. Folate Receptor-Targeting Gold Nanoclusters as Fluorescence Enzyme Mimetic Nanoprobes for Tumor Molecular Colocalization Diagnosis
- Author
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Lintao Cai, Pengfei Zhang, Duyang Gao, Zonghai Sheng, Yanan Wang, Ping Gong, Yifan Ma, Shengtao Fang, and Dehong Hu
- Subjects
Male ,Mice, Nude ,Medicine (miscellaneous) ,Nanoprobe ,Molecular Colocalization ,Gold Nanoclusters ,Fluorescence ,Cell Line ,Nanoclusters ,Mice ,Neoplasms ,medicine ,Animals ,Humans ,Cytotoxicity ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,Peroxidase ,Mice, Inbred BALB C ,Staining and Labeling ,biology ,Histocytochemistry ,Folate Receptors, GPI-Anchored ,Colocalization ,Cancer ,medicine.disease ,Enzyme Mimetic ,Molecular biology ,Enzyme assay ,Biochemistry ,Folate receptor ,biology.protein ,Nanoparticles ,Female ,Tumor Diagnosis ,Gold ,Research Paper - Abstract
Nanoprobes with enzyme-like properties attracted a growing interest in early screening and diagnosis of cancer. To achieve high accuracy and specificity of tumor detection, the design and preparation of enzyme mimetic nanoprobes with high enzyme activity, tumor targeting and excellent luminescence property is highly desirable. Herein, we described a novel kind of fluorescence enzyme mimetic nanoprobe based on folate receptor-targeting Au nanoclusters. The nanoprobes exhibited excellent stability, low cytotoxicity, high fluorescence and enzyme activity. We demonstrated that the nanoprobes could be used for tumor tissues fluorescence/visualizing detection. For the same tumor tissue slice, the nanoprobes peroxidase staining and fluorescent staining were obtained simultaneously, and the results were mutually complementary. Therefore, the fluorescence enzyme mimetic nanoprobes could provide a molecular colocalization diagnosis strategy, efficiently avoid false-positive and false-negative results, and further improve the accuracy and specificity of cancer diagnoses. By examining different clinical samples, we demonstrated that the nanoprobes could distinguish efficiently cancerous cells from normal cells, and exhibit a clinical potential for cancer diagnosis.
- Published
- 2014
7. Indocyanine Green-Loaded Polydopamine-Reduced Graphene Oxide Nanocomposites with Amplifying Photoacoustic and Photothermal Effects for Cancer Theranostics
- Author
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Jingnan Zhang, Haodong Cui, Zonghai Sheng, Dehong Hu, Lintao Cai, and Guanhui Gao
- Subjects
Indocyanine Green ,Materials science ,Indoles ,Theranostic Nanomedicine ,Polymers ,Oxide ,Medicine (miscellaneous) ,Photoacoustic imaging in biomedicine ,Nanotechnology ,Breast Neoplasms ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,law.invention ,Nanocomposites ,Photoacoustic Techniques ,chemistry.chemical_compound ,law ,Animals ,Reduced graphene oxide ,Coloring Agents ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,Mice, Inbred BALB C ,Nanocomposite ,Graphene ,Oxides ,Hyperthermia, Induced ,Photothermal therapy ,Phototherapy ,021001 nanoscience & nanotechnology ,Theranostics ,0104 chemical sciences ,Disease Models, Animal ,Treatment Outcome ,chemistry ,Graphite ,Photoacoustic imaging ,0210 nano-technology ,Indocyanine green ,Research Paper - Abstract
Photoacoustic (PA) imaging and photothermal therapy (PTT) as light-induced theranostic platforms have been attracted much attention in recent years. However, the development of highly efficient and integrated phototheranostic nanoagents for amplifying PA imaging and PTT treatments poses great challenges. Here, we report a novel phototheranostic nanoagent using indocyanine green-loaded polydopamine-reduced graphene oxide nanocomposites (ICG-PDA-rGO) with amplifying PA and PTT effects for cancer theranostics. The results demonstrate that the PDA layer coating on the surface of rGO could effectively absorb a large number of ICG molecules, quench ICG's fluorescence, and enhance the PDA-rGO's optical absorption at 780 nm. The obtained ICG-PDA-rGO exhibits stronger PTT effect and higher PA contrast than that of pure GO and PDA-rGO. After PA imaging-guided PTT treatments, the tumors in 4T1 breast subcutaneous and orthotopic mice models are suppressed completely and no treatment-induced toxicity being observed. It illustrates that the ICG-PDA-rGO nanocomposites constitute a new class of theranostic nanomedicine for amplifying PA imaging and PTT treatments.
- Published
- 2015
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