1. Integrated map of AFLP, SSLP and RFLP markers using a recombinant inbred population of rice (Oryza sativa L.)
- Author
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J. Pot, J. T. M. Groenen, Susan R. McCouch, Yong-Gu Cho, M.-R. Kang, Martin Kuiper, and M. Y. Eun
- Subjects
Genetics ,education.field_of_study ,Oryza sativa ,Population ,food and beverages ,General Medicine ,Biology ,Quantitative trait locus ,Molecular biology ,Gene mapping ,Genetic marker ,Microsatellite ,Amplified fragment length polymorphism ,Restriction fragment length polymorphism ,education ,Agronomy and Crop Science ,Biotechnology - Abstract
A molecular map of rice consisting of 231 amplified fragment length polymorphisms (AFLPs), 212 restriction fragment length polymorphisms (RFLPs), 86 simple-sequence length polymorphisms (SSLPs), five isozyme loci, and two morphological mutant loci [phenol staining of grain (Ph), semi-dwarf habit (sd-1)] has been constructed using an F11 recombinant inbred (RI) population. The mapping population consisted of 164 RI lines and was developed via single-seed descent from an intercross between the genetically divergent parents Milyang 23 (M) (tongil type) and Gihobyeo (G) ( japonica type). A subset of previously mapped RFLP and SSLP markers were used to construct the map framework. The AFLP markers were derived from ten EcoRI(+2) and MseI(+3) primer combinations. All marker types were well distributed throughout the 12 chromosomes. The integrated map covered 1814 cM, with an average interval size of 3.4 cM. The MG map is a cornerstone of the Korean Rice Genome Research Program (KRGRP) and is being continuously refined through the addition of partially sequenced cDNA markers derived from an immature-seed cDNA library developed in Korea, and microsatellite markers developed at Cornell. The population is also being used for quantitative trait locus (QTL) analysis and as the basis for marker-assisted variety development.
- Published
- 1998