Search

Lung tumor is a major disease in Taiwan and the development of minimally invasive imaging to detect and diagnose lung tumor in vivo is of biomedical value. However, prior to in vivo investigation, it is important to obtain the multiphoton signatures associated with lung tumor ex vivo. In this investigation, we used multiphoton microscopy to image normal and cancerous lung tissues (adenocarcinoma). Our results show that a combination of harmonic generation and multiphoton autofluorescence imaging may be used to acquire structural information in discerning lung carcinoma from normal lung tissues.

For many years, the dorsal skinfold chamber has been used for intravital observation in animal models. We have developed a chamber built using medical-grade titanium. Our design allows long-term, high resolution, and large-area observation in nude mice in vivo. Combined with two-photon fluorescence and second-harmonic generation microscopy, our chamber can be used to obtain qualitative and quantitative information for in vivo analysis of diseases such as tumors.