1. The topical use of insulin accelerates the healing of traumatic Tympanic membrane perforations
- Author
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Vani Maria Alves, Maria Célia Jamur, Marcos Miranda de Araújo, Adriana de Andrade Batista Murashima, and Miguel Angelo Hyppolito
- Subjects
Pathology ,medicine.medical_specialty ,biology ,business.industry ,Insulin ,medicine.medical_treatment ,Perforation (oil well) ,Malleus ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Otorhinolaryngology ,medicine ,biology.protein ,Regular insulin ,Immunohistochemistry ,Antibody ,030223 otorhinolaryngology ,Wound healing ,business ,Myofibroblast - Abstract
Objective In recent years, there has been a tendency to search for regulatory substances that can optimize the healing process of perforated tympanic membranes (TMs). The purpose of this study was to determine the effects of using topical insulin on the healing process of traumatic TMs perforations. Study Design Experimental. Methods Tympanic membranes of 20 Wistar rats were perforated in two sections, anterior and posterior to the malleus. The rats were divided into two groups: control and insulin. The insulin group was treated with topical regular insulin. The TMs were histologically examined 3, 5, and 7 days after the perforation through a morphometric analysis of the epithelial layer thickness, perforation size, TM cross-sectional area, semiquantitative and qualitative evaluation of the collagen production by polarization microscopy, and immunohistochemical evaluation of epithelial cells and myofibroblasts markers. Results Insulin accelerated the healing process of the perforated TMs (P
- Published
- 2015
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