1. Alterations of folliculogenesis in women with polycystic ovary syndrome
- Author
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María Belén Hapon, Laura Sícaro, Alicia Beatriz Motta, Graciela A. Jahn, Valeria Analía Sander, and Eduardo Lombardi
- Subjects
endocrine system ,medicine.medical_specialty ,3-Hydroxysteroid Dehydrogenases ,medicine.drug_class ,Endocrinology, Diabetes and Metabolism ,Clinical Biochemistry ,Biochemistry ,Endocrinology ,Aromatase ,Ovarian Follicle ,Internal medicine ,GATA6 Transcription Factor ,Follicular phase ,medicine ,Humans ,RNA, Messenger ,Molecular Biology ,Progesterone ,20-alpha-Hydroxysteroid Dehydrogenase ,biology ,Estradiol ,Reverse Transcriptase Polymerase Chain Reaction ,Cholesterol side-chain cleavage enzyme ,Steroidogenic acute regulatory protein ,Steroid 17-alpha-Hydroxylase ,Cell Biology ,Luteinizing Hormone ,Phosphoproteins ,Polycystic ovary ,GATA4 Transcription Factor ,CYP17A1 ,Estrogen ,Cyclooxygenase 2 ,biology.protein ,Molecular Medicine ,Female ,Folliculogenesis ,Polycystic Ovary Syndrome - Abstract
The objective of the present study was to examine some factors involved in follicular development of women with polycystic ovary syndrome (PCOS). Women with PCOS showed increased levels of serum luteinizing hormone (LH) but decreased follicular production of progesterone and estradiol by pre-ovulatory follicles. The mRNA expression corresponding to steroidogenic acute regulatory protein (StAR), and 20alpha-hydroxysteroid dehydrogenase (20α-HSD) was increased, while that corresponding to cytochrome P450 aromatase (P450arom) was decreased in PCOS follicles as compared to controls. No changes in the mRNA expression for 3beta-hydroxysteroid dehydrogenase 2 (3β-HSD2), cytochrome P450 side-chain cleavage (P450scc), cytochrome P450 17 alpha hydroxylase/lyase (P450c17), cyclooxygenase 2 (COX2), and transcription factors (GATA-4 and GATA-6) were found. We conclude that despite the hyper-luteinized environment of PCOS follicles, these follicles produce lower levels of progesterone and estradiol, and that this is characterized by increased degradation of progesterone and decreased estradiol synthesis. Our data demonstrate that the synthesis of prostaglandin F2α (PGF2α) may be affected in PCOS-follicles and that the transcription factors GATA-4 and GATA-6 are present in PCOS-follicles but they are not involved in the abnormal transcription observed in the steroidogenic enzymes.
- Published
- 2010