1. SARS-CoV-2 Seroprevalence and Antibody Kinetics Among Health Care Workers in a Spanish Hospital After 3 Months of Follow-up
- Author
-
Patricia Sotomayor, Neus Rosell, Antoni Trilla, Alfons Jiménez, Sarah R. Williams, M. Martinez, Angeline Cruz, Gemma Moncunill, Rebeca Santano, Robert A. Mitchell, Pilar Varela, Sergi Sanz, Diana Barrios, Selena Alonso, Montserrat Lamoglia, Javier Rodríguez Moreno, Anna Vilella, Marta Vidal, Alfredo Mayor, Alberto L. García-Basteiro, Laura Puyol, Jochen Hecht, Sara Torres, Nuria Pey, Carlo Carolis, Eugenia Chóliz, Marta Ribes, Susana Méndez, Carlota Dobaño, Antía Figueroa-Romero, Natalia Ortega, Pau Cisteró, Anna Llupià, Silvia Fochs, Sonia Barroso, Chenjerai Jairoce, Marta Tortajada, and Ruth Aguilar
- Subjects
Adult ,Male ,0301 basic medicine ,Immunoglobulin A ,medicine.medical_specialty ,Health Personnel ,Antibodies, Viral ,health care workers ,Immunoglobulin G ,Subclass ,Serology ,03 medical and health sciences ,0302 clinical medicine ,Seroepidemiologic Studies ,Internal medicine ,Major Article ,medicine ,Humans ,antibodies ,Immunology and Allergy ,Seroprevalence ,030212 general & internal medicine ,seroprevalence ,biology ,SARS-CoV-2 ,business.industry ,longitudinal cohort ,COVID-19 ,Middle Aged ,Kinetics ,Cross-Sectional Studies ,AcademicSubjects/MED00290 ,030104 developmental biology ,Infectious Diseases ,Immunoglobulin M ,Seroconversion ,Spain ,Cohort ,biology.protein ,Female ,Antibody ,business ,Follow-Up Studies - Abstract
Background At the COVID-19 spring 2020 pandemic peak in Spain, prevalence of SARS-CoV-2 infection in a cohort of 578 randomly selected health care workers (HCWs) from Hospital Clínic de Barcelona was 11.2%. Methods A follow-up survey 1 month later (April-May 2020) measured infection by rRT-PCR and IgM, IgA, and IgG to the receptor-binding domain of the spike protein by Luminex. Antibody kinetics, including IgG subclasses, was assessed until month 3. Results At month 1, the prevalence of infection measured by rRT-PCR and serology was 14.9% (84/565) and seroprevalence 14.5% (82/565). We found 25 (5%) new infections in 501 participants without previous evidence of infection. IgM, IgG, and IgA levels declined in 3 months (antibody decay rates 0.15 [95% CI, .11–.19], 0.66 [95% CI, .54–.82], and 0.12 [95% CI, .09–.16], respectively), and 68.33% of HCWs had seroreverted for IgM, 3.08% for IgG, and 24.29% for IgA. The most frequent subclass responses were IgG1 (highest levels) and IgG2, followed by IgG3, and only IgA1 but no IgA2 was detected. Conclusions Continuous and improved surveillance of SARS-CoV-2 infections in HCWs remains critical, particularly in high-risk groups. The observed fast decay of IgA and IgM levels has implications for seroprevalence studies using these isotypes., SARS-CoV-2 prevalence in 578 health care workers increased from 11.2% at the COVID-19 pandemic peak to 14.9% 1 month later. IgM, IgG, and IgA levels declined over 3 months of follow-up with 68.33%, 3.08%, and 24.29% of seroreversions, respectively.
- Published
- 2020