1. Mapuera virus, a rubulavirus that inhibits interferon signalling in a wide variety of mammalian cells without degrading STATs
- Author
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Nicola Stock, Steve Goodbourn, B. Precious, Kathrin Hagmaier, Lin-Fa Wang, Richard E. Randall, and Kay Childs
- Subjects
Cytoplasm ,Molecular Sequence Data ,Active Transport, Cell Nucleus ,Transcription factor complex ,Electrophoretic Mobility Shift Assay ,Virus ,Cell Line ,03 medical and health sciences ,Viral Proteins ,Interferon ,Virology ,medicine ,Animals ,Humans ,Electrophoretic mobility shift assay ,Amino Acid Sequence ,Rubulavirus ,Phosphorylation ,Mononegavirales ,030304 developmental biology ,Cell Nucleus ,0303 health sciences ,biology ,Base Sequence ,Animal ,030302 biochemistry & molecular biology ,Interferon-Stimulated Gene Factor 3 ,biology.organism_classification ,3. Good health ,STAT Transcription Factors ,Microscopy, Fluorescence ,STAT protein ,Interferons ,medicine.drug - Abstract
Mapuera virus(MPRV) is a paramyxovirus that was originally isolated from bats, but its host range remains unknown. It was classified as a member of the genusRubulaviruson the basis of structural and genetic features. Like other rubulaviruses it encodes a V protein (MPRV/V) that functions as an interferon (IFN) antagonist. Here we show that MPRV/V differs from the IFN antagonists of other rubulaviruses in that it does not induce the proteasomal degradation of STAT proteins, key factors in the IFN signalling cascade. Rather, MPRV/V prevents the nuclear translocation of STATs in response to IFN stimulation and inhibits the formation of the transcription factor complex ISGF3. We also show that MPRV/V blocks IFN signalling in cells from diverse mammalian species and discuss the IFN response as a barrier to cross-species infections.
- Published
- 2007