1. F-actin Distribution of Dictyostelium Myosin I Double Mutants
- Author
-
Margaret A. Titus and Michelle D. Peterson
- Subjects
Genetics ,genetic structures ,biology ,Mutant ,Wild type ,macromolecular substances ,Myosins ,biology.organism_classification ,Microbiology ,Dictyostelium ,Actins ,eye diseases ,Dictyostelium discoideum ,Cell biology ,Mutation ,Myosin ,Animals ,Pseudopodia ,Cytoskeleton ,Gene knockout ,Actin - Abstract
The roles of the myosin I class of mechanoenzymes have been investigated by single and double gene knockout studies in the amoeba Dictyostelium discoideum. Cells lacking different myosin I pairs (myoA-/myoB-, myoB-/myoC-, and myoA-/myoC-) were examined with respect to their cytoskeletal organization. F-actin localization by rhodamine-phalloidin staining of cells indicates that the myoA-/myoB-, myoB-/myoC-, and myoA-/myoC- cells appear to redistribute their F-actin more slowly than wild type cells upon adhesion to a substrate. These studies suggest that Dictyostelium myoA, myoB, and myoC may have overlapping roles in maintaining the integrity or organization of the cortical membrane cytoskeleton.
- Published
- 1994
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