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1. Interaction of link protein with collagen

Author

S, Chandrasekhar, H K, Kleinman, and J R, Hassell

Subjects
Paper, Extracellular Matrix Proteins, Protein Denaturation, Macromolecular Substances, Immunologic Techniques, Animals, Collodion, Proteins, Enzyme-Linked Immunosorbent Assay, Proteoglycans, Collagen, Protein Binding, Rats
Abstract

Link protein (Mr = 42,000) is an integral component of cartilage as well as of some noncartilagenous tissues. In cartilage, it forms a macromolecular complex with cartilage proteoglycan and hyaluronic acid, but its function in other tissues is unknown. We provide evidence here that the link protein of cartilage binds well to native collagen types I and III. The binding occurs only if both link protein and collagen are native. The binding of link protein to collagen type fibrils is higher than to monomeric collagen. Link protein binding to collagen fibrils is saturable and occurs at molar ratio of collagen to link protein of 7-13:1. These data suggest that the link protein binds to collagen and that the binding requires the collagen to be in its native triple helical structure. This interaction may play a role in collagen fibril formation.

Published
1983

2. Affinity purification of antibodies from diazotized paper blots of heterogeneous protein samples

Author

J B, Olmsted

Subjects
Paper, Mice, Neuroblastoma, Tubulin, Cricetinae, Animals, Fluorescent Antibody Technique, Indicators and Reagents, Diazonium Compounds, Fibroblasts, Microtubules, Antibodies, Cell Line
Abstract

A method is described for the affinity purification of antibodies using protein samples that have been electrophoretically transferred to diazotized paper. Using differentiated neuroblastoma cells as the protein sample and a heterogeneous anti-microtubule protein serum, antibodies were isolated that specifically bound only to tubulin on blots and that stained microtubule networks in cells. The general utility of this method for various types of applications is discussed.

Published
1981

3. Metabolism of basic amino acids in Pseudomonas putida. Intermediates in L-arginine catabolism

Author

D L, Miller and V W, Rodwell

Subjects
Electrophoresis, Paper, Hot Temperature, Chemical Phenomena, Chromatography, Paper, Malates, Arginine, Tritium, Guanidines, Canavanine, Drug Stability, Ammonia, Pseudomonas, Urea, Carbon Isotopes, Computers, Aminobutyrates, Hydrogen-Ion Concentration, Amides, Keto Acids, Butyrates, Chemistry, Kinetics, Enzyme Induction, Citrulline, Chromatography, Thin Layer
Published
1971

4. Arginine racemase of Pseudomonas graveolens. II. Racemization and transamination of ornithine catalyzed by arginine racemase

Author

T, Yorifuji, H, Misono, and K, Soda

Subjects
Electrophoresis, Ornithine, Paper, Light, Oxaloacetates, Rotation, Phenylpyruvic Acids, Ultraviolet Rays, Acetates, Arginine, Models, Biological, Catalysis, Organophosphorus Compounds, Pseudomonas, Amino Acids, Isomerases, Pyruvates, Transaminases, Alanine, Aminobutyrates, Stereoisomerism, Hydrogen-Ion Concentration, Keto Acids, Enzyme Activation, Kinetics, Spectrophotometry, Pyridoxal Phosphate, Picolines, Ketoglutaric Acids, Propionates, Dialysis, Protein Binding
Published
1971

5. Sequence of bovine liver glutamate dehydrogenase. IV. Cyanogen bromide peptides

Author

T J, Langley and E L, Smith

Subjects
Electrophoresis, Paper, Cyanides, Chemical Phenomena, Chromatography, Paper, Hydrolysis, Carboxypeptidases, Chromatography, Ion Exchange, Chemistry, Methionine, Glutamate Dehydrogenase, Liver, Chromatography, Gel, Animals, Cattle, Trypsin, Amino Acid Sequence, Chromatography, Thin Layer, Amino Acids, Peptides, Countercurrent Distribution
Published
1971

6. Purification and specificity of porcine enterokinase

Author

S, Maroux, J, Baratti, and P, Desnuelle

Subjects
Electrophoresis, Paper, Isoflurophate, Chromatography, Paper, Duodenum, Swine, Aminopeptidases, Methylation, Catalysis, Chromatography, DEAE-Cellulose, Endopeptidases, Animals, Chymotrypsin, Protease Inhibitors, Trypsin, Isoleucine, Aspartic Acid, Enzyme Precursors, Binding Sites, Hydrolysis, Lysine, Phosphorus Isotopes, Hydrogen-Ion Concentration, Chromatography, Ion Exchange, Enzyme Activation, Kinetics, Chromatography, Gel, Trypsinogen, Calcium, Cattle, Sulfonic Acids, Peptides, Trypsin Inhibitors, Toluene
Published
1971

8. Control of pyrimidine biosynthesis in human lymphocytes. Induction of glutamine-utilizing carbamyl phosphate synthetase and operation of orotic acid pathway during blastogenesis

Author

K, Ito and H, Uchino

Subjects
Adult, Electrophoresis, Paper, Chromatography, Paper, Uracil Nucleotides, Glutamine, Anhydrides, Cell Line, Glutamates, Culture Techniques, Lectins, Chemical Precipitation, Humans, Phosphoric Acids, Lymphocytes, Uracil, Orotic Acid, Carbon Isotopes, Sulfates, Hydrolysis, Phosphotransferases, Cell Differentiation, Carbon Dioxide, Chromatography, Ion Exchange, Quaternary Ammonium Compounds, Pyrimidines, Enzyme Induction, Dactinomycin, Citrulline, Puromycin, Carbamates
Published
1971

10. The formation of uridine diphosphate-glucuronic acid in plants. Uridine diphosphate-glucuronic acid pyrophosphorylase from barley seedlings

Author

R M, Roberts

Subjects
Electrophoresis, Paper, Time Factors, Chromatography, Paper, Uracil Nucleotides, Glucuronates, Chromatography, DEAE-Cellulose, Calcium Chloride, Chlorides, Chemical Precipitation, Magnesium, Phosphoric Acids, Carbon Isotopes, Manganese, Nucleoside Diphosphate Sugars, Sulfates, Cobalt, Hydrogen-Ion Concentration, Plants, Chromatography, Ion Exchange, Nucleotidyltransferases, Quaternary Ammonium Compounds, Alcohol Oxidoreductases, Kinetics, Zinc, Glucose, Chromatography, Gel, Edible Grain, Copper
Published
1971

11. Phosphoribokinase from Pseudomonas saccharophila

Author

L R, DeChatelet and J B, Alpers

Subjects
Electrophoresis, Paper, Carbon Isotopes, Kinetics, Pentosephosphates, Drug Stability, Phosphoglucomutase, Pseudomonas, Phosphotransferases, Hexosephosphates, Hydrogen-Ion Concentration, Chromatography, Ion Exchange, Vibration
Published
1970

12. Rotational relaxation time of pyridoxyl 5-phosphate lysozyme

Author

R, Irwin and J E, Churchich

Subjects
Electrophoresis, Paper, Alkylation, Chemical Phenomena, Light, Rotation, Chromatography, Paper, Borohydrides, Acetates, Fluorescence, Micrococcus, Cell Wall, Fluorometry, Trypsin, Glucosamine, Chemistry, Physical, Viscosity, Hydrolysis, Lysine, Sodium, Iodides, Fluoresceins, Chemistry, Spectrophotometry, Pyridoxal Phosphate, Picolines, Chromatography, Gel, Potassium, Muramidase, Peptides, Oxidation-Reduction, Mathematics, Thiocyanates
Published
1971

15. Enzymatic breakage and joining of deoxyribonucleic acid. IX. Synthesis and properties of the deoxyribonucleic acid adenylate in the phage T4 ligase reaction

Author

C L, Harvey, T F, Gabriel, E M, Wilt, and C C, Richardson

Subjects
Electrophoresis, Genetics, Microbial, Paper, Chemical Phenomena, Morpholines, Deoxyribonucleotides, Polynucleotides, Tritium, Coliphages, Chromatography, DEAE-Cellulose, Ligases, Adenosine Triphosphate, Drug Stability, Centrifugation, Density Gradient, Escherichia coli, Thymine Nucleotides, Adenine Nucleotides, Hydrolysis, Phosphotransferases, Temperature, Phosphorus Isotopes, Hydrogen-Ion Concentration, Alkaline Phosphatase, Guanine Nucleotides, Chemistry, DNA, Viral, Mutation, Chromatography, Gel
Published
1971

16. Mechanism of action of sucrose phosphorylase. 3. The reaction with water and other alcohols

Author

J J, Mieyal, M, Simon, and R H, Abeles

Subjects
Paper, Carbon Isotopes, Chromatography, Protein Denaturation, Sucrose, Binding Sites, Chromatography, Gas, Ethanol, Hydrolysis, Methanol, Water, Acetates, Dioxins, Catalysis, Phosphates, Glycols, Kinetics, Structure-Activity Relationship, Drug Stability, Cyclohexanes, Glucosyltransferases, Pseudomonas, Nitriles, Protein Binding
Published
1972

17. Ethanolamine deaminase, a cobamide coenzyme-dependent enzyme. II. Physical and chemical properties and interaction with cobamides and ethanolamine

Author

B H, Kaplan and E R, Stadtman

Subjects
Electrophoresis, Paper, Carbon Isotopes, Cyanides, Chemical Phenomena, Chromatography, Paper, Ultraviolet Rays, Spectrum Analysis, Detergents, Coenzymes, Acetaldehyde, Electrophoresis, Disc, Amino Alcohols, Guanidines, Molecular Weight, Chemistry, Vitamin B 12, Aminohydrolases, Urea, Cysteine, Sulfhydryl Compounds, Amino Acids, Ultracentrifugation, Nitrobenzenes, Protein Binding
Published
1968

18. Measurement of cyclic 3',5'-denosine monophosphate by the activation of skeletal muscle protein kinase

Author

W B, Wastila, J T, Stull, S E, Mayer, and D A, Walsh

Subjects
Male, Paper, Time Factors, Epinephrine, In Vitro Techniques, Norepinephrine, Adenosine Triphosphate, Dogs, Cyclic AMP, Methods, Animals, Magnesium, Trichloroacetic Acid, Epididymis, Adenine Nucleotides, Tissue Extracts, Microchemistry, Muscles, Myocardium, Phosphotransferases, Isoproterenol, Caseins, Phosphorus Isotopes, Heart, Glucagon, Phosphoric Monoester Hydrolases, Rats, Enzyme Activation, Adipose Tissue, Liver, Rabbits, Liver Extracts, Adenylyl Cyclases
Published
1971

21. The chemical structure of tryptophanase from Escherichia coli. I. Isolation and structure of a pyridoxyl decapeptide from borohydride-reduced holotryptophanase

Author

H, Kagamiyama, Y, Morino, and E E, Snell

Subjects
Boron Compounds, Electrophoresis, Paper, Indoles, Chromatography, Paper, Lysine, Tryptophan, Chromatography, Ion Exchange, Methylation, Fluorescence, Spectrophotometry, Escherichia coli, Chymotrypsin, Amino Acids, Peptides, Pyruvates, Oxidation-Reduction, Hydro-Lyases
Published
1970

22. Metabolism of cyclic adenosine 3',5'-monophosphate-8-14C by isolated, perfused rat liver

Author

R A, Levine, S E, Lewis, J, Shulman, and A, Washington

Subjects
Electrophoresis, Male, Paper, Carbon Isotopes, Cell Membrane Permeability, Time Factors, Adenine Nucleotides, Chromatography, Paper, Biological Transport, Fasting, Carbon Dioxide, In Vitro Techniques, Chromatography, Ion Exchange, Rats, Perfusion, Blood, Liver, Spectrophotometry, Cyclic AMP, Animals, Autoradiography, Bile
Published
1969

24. A phytotoxic glycopeptide from potato plants infected with Corynebacterium sepedonicum

Author

G A, Strobel

Subjects
Electrophoresis, Paper, Infrared Rays, Immune Sera, Monosaccharides, Corynebacterium, Hydrogen-Ion Concentration, Plants, Gluconates, Molecular Weight, X-Ray Diffraction, Spectrophotometry, Chromatography, Gel, Animals, Biological Assay, Indicators and Reagents, Rabbits, Amino Acids, Peptides, Acids, Gels, Toxins, Biological
Published
1970

25. Evidence for a covalent linkage between heteropolysaccharide and an hydroxy proline-containing peptide from Metridium dianthus connective tissue

Author

R L, Katzman and A L, Oronsky

Subjects
Electrophoresis, Paper, Chromatography, Gas, Chromatography, DEAE-Cellulose, Potassium Chloride, Cnidaria, Species Specificity, Methods, Animals, Chemical Precipitation, Amino Acid Sequence, Amino Acids, Fucose, Clostridium, Glucosamine, Xylose, Glycopeptides, Galactose, Hexosamines, Pepsin A, Hydroxyproline, Glucose, Hydrazines, Microbial Collagenase, Connective Tissue, Chromatography, Gel, Chromatography, Thin Layer, Collagen, Sulfonic Acids, Mannose, Peptide Hydrolases, Toluene
Published
1971

26. Steroid-protein interactions. XVI. Isolation and characterization of the corticosteroid-binding globulin of the rabbit

Author

G J, Chader and U, Westphal

Subjects
Electrophoresis, Male, Paper, Hydrocortisone, Guinea Pigs, Carbohydrates, Dogs, Species Specificity, Alpha-Globulins, Animals, Humans, Amino Acids, Immunoelectrophoresis, Transcortin, Carbon Isotopes, Chromatography, Ion Exchange, Rats, Molecular Weight, Cats, Chromatography, Gel, Cattle, Female, Rabbits, Corticosterone, Dialysis, Ultracentrifugation, Protein Binding
Published
1968

27. Rapid labeling of mitochondrial lipids by labeled orthophosphate and adenosine triphosphate

Author

A K, Hajra, E B, Seguin, and B W, Agranoff

Subjects
Electrophoresis, Paper, Antimetabolites, Chromatography, Paper, Guinea Pigs, Phosphotransferases, Phosphorus Isotopes, Mitochondria, Liver, In Vitro Techniques, Hydroxylamines, Lipid Metabolism, Phosphatidylinositols, Oxidative Phosphorylation, Phosphoric Monoester Hydrolases, Phosphates, Adenosine Triphosphate, Animals, Oligomycins, Glycosides, Dinitrophenols, Phospholipids
Published
1968

28. The deoxyribonuclease induced after infection of Escherichia coli by bacteriophage T5. I. Characterization of the enzyme as a 5'-exonuclease

Author

G D, Frenkel and C C, Richardson

Subjects
Electrophoresis, Genetics, Microbial, Paper, Chromatography, Paper, Ultraviolet Rays, Polynucleotides, Nucleic Acid Denaturation, Coliphages, Models, Biological, Chromatography, DEAE-Cellulose, Ligases, Centrifugation, Density Gradient, Escherichia coli, Animals, Magnesium, Carbon Isotopes, Chromatography, Deoxyribonucleases, Nucleotides, Venoms, Hydrolysis, Phosphorus Isotopes, Snakes, DNA, Alkaline Phosphatase, Chromatography, Ion Exchange, Phosphoric Monoester Hydrolases, Radiation Effects, Enzyme Induction, DNA Nucleotidyltransferases, DNA, Viral, Mutation, Chromatography, Thin Layer, Hydroxyapatites, Mathematics
Published
1971

29. Enzymatic modification of proteins. VI. Site of acylation of bovine serum albumin in the leucine, phenylalanine-transfer reaction

Author

M J, Leibowitz and R L, Soffer

Subjects
Electrophoresis, Paper, Carbon Isotopes, Chemical Phenomena, Chromatography, Paper, Acylation, Phenylalanine, Receptors, Drug, Serum Albumin, Bovine, Arginine, Chromatography, Ion Exchange, Tritium, Pepsin A, Chemistry, Liver, RNA, Transfer, Solubility, Leucine, Endopeptidases, Escherichia coli, Animals, Cattle, Trypsin, Peptides, Acyltransferases
Published
1971

31. Cystathionine gamma-synthase from Salmonella. Beta elimination and replacement reactions and inhibition by O-succinylserine

Author

S, Guggenheim and M, Flavin

Subjects
Electrophoresis, Paper, Salmonella typhimurium, Chromatography, L-Serine Dehydratase, Hot Temperature, Chromatography, Paper, Succinates, Hydrogen-Ion Concentration, Chromatography, Ion Exchange, Kinetics, Chromatography, Gel, Methods, Serine, Chemical Precipitation, Hydroxyapatites, Amino Acids, Cellulose, Hydro-Lyases
Published
1969

32. Biosynthesis of plasma lipoproteins by rat liver ribosomes

Author

J J, ungenberg de Jong and J B, Marsh

Subjects
Electrophoresis, Male, Paper, Carbon Isotopes, Immunodiffusion, Immune Sera, Lipoproteins, Mitochondria, Liver, Kidney, Tritium, Antibodies, Anti-Idiotypic, Rats, Antigen-Antibody Reactions, Liver, Solubility, Protein Biosynthesis, Centrifugation, Density Gradient, Animals, Rabbits, Amino Acids, Peptides, Ribosomes, Protein Binding
Published
1968

34. The amino acid sequence of a kappa type Bence-Jones protein. I. Tryptic peptides

Author

K, Titani, E J, Whitley, and F W, Putnam

Subjects
Electrophoresis, Male, Paper, Autoanalysis, Alkylation, Chemical Phenomena, Chromatography, Paper, Hydrogen-Ion Concentration, Chromatography, Ion Exchange, Chemistry, Solubility, Methods, Humans, Trypsin, Amino Acid Sequence, Amino Acids, Multiple Myeloma, Peptides, Nitrobenzenes, Aged, Bence Jones Protein
Published
1969

35. Composition, properties, and substrate specificities of Myxobacter AL-1 protease

Author

R L, Jackson and R S, Wolfe

Subjects
Electrophoresis, Paper, Immunodiffusion, Alkylation, Chemical Phenomena, Chromatography, Paper, Ultraviolet Rays, Iodoacetates, Phosphates, Chymotrypsin, Insulin, Trypsin, Amino Acids, Hexoses, Autoanalysis, Bacteria, Chemistry, Physical, Viscosity, Spectrum Analysis, Tryptophan, Electrophoresis, Disc, Molecular Weight, Chromatography, Gel, Cystine, Peptides, Ultracentrifugation, Peptide Hydrolases
Published
1968

36. Structural analysis of nucleolar precursors of ribosomal ribonucleic acid. Polypurine sequences in nucleolar 28 S ribonucleic acid of Novikoff hepatoma ascites cells

Author

S, Seeber, Y C, Choi, and H, Busch

Subjects
Electrophoresis, Male, Paper, Carcinoma, Hepatocellular, Uracil Nucleotides, Polynucleotides, Cytosine Nucleotides, Chromatography, DEAE-Cellulose, Phosphates, Ribonucleases, Centrifugation, Density Gradient, Animals, Ascitic Fluid, RNA, Neoplasm, Pancreas, Base Sequence, Liver Neoplasms, Phosphorus Isotopes, Neoplasms, Experimental, Hydrogen-Ion Concentration, Chromatography, Ion Exchange, Phosphoric Monoester Hydrolases, Rats, RNA, Ribosomal, Autoradiography, Cell Nucleolus, Spleen
Published
1971

38. Formaldehyde-induced cross-linkages in the subunit of the Escherichia coli tryptophan synthetase

Author

J S, Myers and J K, Hardman

Subjects
Electrophoresis, Paper, Indoles, Chemical Phenomena, Chromatography, Paper, Glutamine, Formaldehyde, Escherichia coli, Serine, Chymotrypsin, Trypsin, Amino Acid Sequence, Amino Acids, Hydro-Lyases, Carbon Isotopes, Binding Sites, Lysine, Tryptophan, Electrophoresis, Disc, Pepsin A, Molecular Weight, Chemistry, Kinetics, Glycerophosphates, Chromatography, Gel, Asparagine, Peptides
Published
1971

40. Cyanide metabolism by Bacillus megaterium

Author

P A, Castric and G A, Strobel

Subjects
Electrophoresis, Paper, Radioisotopes, Aspartic Acid, Carbon Isotopes, Cyanides, Cell-Free System, Chromatography, Paper, Carbon Dioxide, Models, Biological, Culture Media, Bacillus megaterium, Serine, Amino Acids, Asparagine, Soil Microbiology
Published
1969

41. The amino acid sequence of the Q coat protein

Author

T, Maita and W, Konigsberg

Subjects
Electrophoresis, Paper, Alkylation, Iodoacetates, Carboxypeptidases, Naphthalenes, Chromatography, Ion Exchange, Coliphages, Chromatography, DEAE-Cellulose, Viral Proteins, Acetamides, Endopeptidases, Chymotrypsin, Trypsin, Amino Acid Sequence, Amino Acids, Sulfonic Acids, Peptides, Oxidation-Reduction, Dinitrophenols
Published
1971