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The therapeutic goals are palliative for metastatic breast cancer (MBC) and include prolongation of survival with good quality of life (QoL) and symptom control. The purpose of this study was to examine QoL among women with MBC treated on KCSG-BR07-02 with maintenance of paclitaxel plus gemcitabine (PG) chemotherapy after achieving disease control to initial six cycles of PG chemotherapy or observation. Patients were randomized to either maintenance chemotherapy or observation until progression. QoL was assessed using EORTC QLQ-C30 and BR-23. QoL at each cycle was compared between the two treatment arms using the 2-sample t test. Generalized estimating equation method was used to examine the overall difference between the two treatments in QoL. All reported p-values are 2 sided. There were no statistically significant differences between two arms in most of the component of the EORTC QLQ-C30 and BR-23 (p > 0.05). There was no significant difference between two treatments (p = 0.6094 for QLQ-C30, p = 0.5516 for BR23) at baseline, and there did not exist significant change over the cycle (p = 0.0914 for QLQ-C30, p = 0.7981 for BR23). There was no significant interaction effect between treatment and cycle (p = 0.5543 for QLQ-C30. p = 0.5817 for BR23). Maintenance PG chemotherapy in patients with MBC achieving disease control with an initial six cycles of PG chemotherapy resulted in better PFS and OS compared to observation without impeding QoL.

compounds with anticancer and antitumor activity. Onchidium is an airbreathing and shell-less sea slug from gastropod mollusc in the family Onchidiidae, that is distributed with a good abundance in Persian Gulf. Methods: Adult Onchidium were collected from Persian Gulf during spring of 2010. Eggs were freeze dried and used for protein extraction by homogenization in acid acetic (0.1N). After centrifugation, supernatants were freeze dried. Freeze dried extract dissolved in ammonium acetate buffer and it was subjected to gel filtration on a Sephadex G50 column (1.4×60). Purified molecules were separated by SDS-PAGE under denaturing conditions in 12.5% polyacrylamide gels which was calibrated with SDS-PAGE polypeptide high range standards. A 320 kDa protein separated and their in vitro anti-proliferative and cytotxic effects on human breast cancer cell line (T47D in RPMI-1640 culture medium) examined by MTT assay. Results: This protein showed growth inhibitory activity against T47D cells with LC50 values ranging 0.240 to 0.923 nM. This result suggests that separated 320 kDa protein has potent activity against breast cancer cell line. To understand the mechanism of their anti-cancer action, the effect of this compound on the location of cells in the cell cycle was examined using fluorescent activated cell-sorting analysis. This protein was added to T47D culture medium at varying doses, and then at 24 hr the cells were examined for cell cycle location. The addition of separated 320 kDa protein to these cells caused a dose-dependent block in the cell cycle in the G2/M phase. Conclusion: Although these primeval results showed anti-proliferative capacity of this protein, the modulation of apoptotic pathways deserves further evaluation as an anticancer strategy. Disclosure of Interest: None Declared