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Your search keyword '"Mie, Masayasu"' showing total 9 results
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9 results on '"Mie, Masayasu"'

2. Development of a homogeneous immunoassay system using protein A fusion fragmented Renilla luciferase

Author

mie, masayasu, Ngo, P. B. T., and KOBATAKE, EIRY

Subjects
Immunoassay, Fusion, biology, medicine.diagnostic_test, Chemistry, Recombinant Fusion Proteins, Biochemistry, Fusion protein, Molecular biology, Antibodies, Bacterial, Peptide Fragments, Analytical Chemistry, Protein Structure, Tertiary, Homogeneous, Electrochemistry, biology.protein, Biophysics, medicine, Escherichia coli, Environmental Chemistry, Protein A, Staphylococcal Protein A, Spectroscopy, Luciferases, Renilla
Abstract

A homogeneous immunoassay system was developed using fragmented Renilla luciferase (Rluc). The B domain of protein A was fused to two Rluc fragments. When complexes between an antibody and fragmented Rluc fusion proteins bind to target molecules, the Rluc fragments come into close proximity and the luminescence activity of fragmented Rluc is restored by complementation. As proof-of-principle, this fragmented Rluc system was used to detect E. coli homogeneously using an anti-E. coli antibody.

Published
2011

3. Selection of DNA aptamers recognizing small cell lung cancer using living cell-SELEX

Author

Kunii, T., Ogura, S., mie, masayasu, and KOBATAKE, EIRY

Subjects
Lung Neoplasms, Confocal, Aptamer, Cell, Biochemistry, Analytical Chemistry, Flow cytometry, law.invention, chemistry.chemical_compound, Confocal microscopy, law, Electrochemistry, medicine, Environmental Chemistry, Humans, A-DNA, neoplasms, Spectroscopy, Microscopy, Confocal, medicine.diagnostic_test, Base Sequence, Chemistry, SELEX Aptamer Technique, Aptamers, Nucleotide, Flow Cytometry, Molecular biology, Small Cell Lung Carcinoma, humanities, respiratory tract diseases, medicine.anatomical_structure, Systematic evolution of ligands by exponential enrichment, DNA, Fluorescein-5-isothiocyanate
Abstract

We applied Systematic Evolution of Ligands by EXponential enrichment using Small Cell Lung Cancer (SCLC) cells. A DNA aptamer was identified and evaluated by fluorescent confocal microscopy and flow cytometry. Our results showed that the DNA aptamer binds to molecules that exist predominantly on target SCLC cell surfaces compared with other types of SCLC cells.

Published
2011

4. Construction of a functional IgG-binding luciferase fusion protein for the rapid detection of specific bacterial strains

Author

Nakamura, M., mie, masayasu, and KOBATAKE, EIRY

Subjects
chemistry.chemical_classification, G protein, Chemistry, Binding protein, Recombinant Fusion Proteins, Plasma protein binding, Biochemistry, Molecular biology, Fusion protein, Analytical Chemistry, Enzyme, Spectrometry, Fluorescence, Bacterial Proteins, IgG binding, Immunoglobulin G, Electrochemistry, Escherichia coli, Environmental Chemistry, Bioluminescence, Luciferase, Luciferases, Spectroscopy, Protein Binding
Abstract

A fusion protein consisting of two IgG-binding domains of streptococcal protein G and firefly luciferase was constructed, and a simple and specific bioluminescent immunodetection system for bacterial strains was developed.

Published
2010

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