1. Cetirizine as pH-dependent cross-reactant in a carbamazepine-specific immunoassay
- Author
-
Ulrich Panne, Jana Falkenhagen, Rudolf J. Schneider, Michael G. Weller, and Arnold Bahlmann
- Subjects
Chromatography ,medicine.diagnostic_test ,Chemistry ,Water pollutants ,Ph dependent ,Enzyme-Linked Immunosorbent Assay ,Carbamazepine ,Hydrogen-Ion Concentration ,Biochemistry ,High-performance liquid chromatography ,Cetirizine ,Analytical Chemistry ,Cross-Linking Reagents ,Wastewater ,Immunoassay ,Electrochemistry ,medicine ,Environmental Chemistry ,Spectroscopy ,Chromatography, High Pressure Liquid ,Water Pollutants, Chemical ,medicine.drug - Abstract
High performance liquid chromatography (HPLC) was hyphenated with a previously reported carbamazepine-specific enzyme-linked immunosorbent assay (ELISA) as a screening approach to water analysis in order to identify possible interferences from transformation products. Treated wastewater was analysed and three substances were recognized by the antibody besides carbamazepine: the metabolites 10,11-dihydro-10,11-epoxycarbamazepine and 2-hydroxycarbamazepine plus the structurally not obviously related antihistamine cetirizine. The molar cross-reactivity against cetirizine was found to be pH-dependent and assessed to be 400% at pH 4.5 and 22% at pH 10.5. Performing the ELISA at pH 10.5 greatly improved the accuracy when carbamazepine was determined in surface and wastewater samples.
- Published
- 2011