18 results on '"Moscone D"'
Search Results
2. Amperometric lysine bioprobes analysis in feeds
- Author
-
Lavagnini, M.G., primary, Moscone, D., additional, Palleschi, G., additional, Compagnone, D., additional, and Cremisini, C., additional
- Published
- 1993
- Full Text
- View/download PDF
3. Subcutaneous microdialysis probe coupled with glucose biosensor for in vivo continuous monitoring
- Author
-
MOSCONE, D, primary, PASINI, M, additional, and MASCINI, M, additional
- Published
- 1992
- Full Text
- View/download PDF
4. Paper-based synthesis of Prussian Blue Nanoparticles for the development of whole blood glucose electrochemical biosensor
- Author
-
Danila Moscone, Fabiana Arduini, Roberto Cusenza, Stefano Cinti, Cinti, S., Cusenza, R., Moscone, D., and Arduini, F.
- Subjects
Blood Glucose ,Paper ,Surface Properties ,Reducing agent ,Paper-based ,Electrode ,Surface Propertie ,Nanoparticle ,Nanotechnology ,Biosensing Techniques ,02 engineering and technology ,01 natural sciences ,Analytical Chemistry ,Biosensing Technique ,chemistry.chemical_compound ,Settore CHIM/01 - Chimica Analitica ,Glucose oxidase ,Particle Size ,Hydrogen peroxide ,Electrodes ,Prussian blue ,Electrochemical Technique ,biology ,Filter paper ,010401 analytical chemistry ,Electrochemical Techniques ,Hydrogen-Ion Concentration ,021001 nanoscience & nanotechnology ,BiosensorPaper-basedPoint-of-carePrussian Blue NanoparticlesScreen-printed electrodesWhole blood ,Environmentally friendly ,0104 chemical sciences ,Whole blood ,Prussian Blue Nanoparticle ,chemistry ,Point-of-care ,Screen-printed electrode ,biology.protein ,Nanoparticles ,0210 nano-technology ,Biosensor ,Ferrocyanide ,Ferrocyanides - Abstract
Nowadays, environmentally friendly synthesis pathways for preserving the environment and minimizing waste are strongly required. Herein, we propose filter paper as a convenient scaffold for chemical reactions. To demonstrate this novel approach, Prussian Blue Nanoparticles (PBNPs) were synthesized on filter paper by utilizing few μL of its precursors without external inputs, i.e. pH, voltage, reducing agents, and without producing waste as well. The functional paper, named “Paper Blue”, is successfully applied in the sensing field, exploiting the reduction of hydrogen peroxide at low applied potential. The eco-designed “Paper Blue” was combined with wax- and screen-printing to manufacture a reagentless electrochemical point-of-care device for diabetes self-monitoring, by using glucose oxidase as the biological recognition element. Blood glucose was linearly detected for a wide concentration range up to 25 mM (450 mg/dL), demonstrating its suitability for management of diabetes and glucose-related diseases. The Paper Blue-based biosensor demonstrated a correlation coefficient of 0.987 with commercial glucose strips (Bayer Contour XT). The achieved results demonstrated the effectiveness of this approach, which is also extendible to other (bio)systems to be applied in catalysis, remediation, and diagnostics.
- Published
- 2018
5. How to extend range linearity in enzyme inhibition-based biosensing assays
- Author
-
Giuseppe Palleschi, Danila Moscone, Aziz Amine, Stefano Cinti, Fabiana Arduini, Amine, A., Cinti, S., Arduini, F., Moscone, D., and Palleschi, G.
- Subjects
02 engineering and technology ,Biosensing Techniques ,Mass spectrometry ,01 natural sciences ,Analytical Chemistry ,chemistry.chemical_compound ,Biosensing Technique ,Extended linearity ,Bioassay ,Enzyme Inhibitor ,Settore CHIM/01 - Chimica Analitica ,Cholinesterase Inhibitor ,Enzyme Inhibitors ,Chromatography ,Chemistry ,010401 analytical chemistry ,Linearity ,Substrate (chemistry) ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Enzyme inhibition ,Kinetics ,Linear range ,Progress curve ,Reagent ,Costs and Cost Analysi ,enzyme inhibition, progress curve, extended linearity, substrate conversion ,Acetylcholinesterase ,Costs and Cost Analysis ,Substrate conversion ,Cholinesterase Inhibitors ,0210 nano-technology ,Biosensor ,Fluoride - Abstract
Bioassays based on enzyme inhibition are analytical tools widely employed for inhibitor analysis. Beside the conventional analytical techniques such as chromatography and mass spectrometry, these bioassays are cost-effective, easy to use, and suitable for in situ measurement but they are often characterised by a quite narrow linear range. Herein, we report a novel graphical method based on integrated Michaelis-Menten equation, valid for all types of reversible inhibition, which provides an extended linear range. The suitability of this innovative approach was demonstrated in the case of fluoride quantification using a colorimetric bioassay based on acetylcholinesterase inhibition. The “half time reaction”, estimated by the progress curve of cholinesterase inhibition, was plotted versus the fluoride inhibitor concentration, observing an extended linear range up to 5 mM, instead of 0.6 mM using initial rate measurements. The applicability of this new concept was further demonstrated in the case of catalase enzyme inhibited by cyanide. Furthermore, it was demonstrated that fixed substrate conversion at level of 10–50% allows determination of inhibitor concentration in a wide linear range with high precision and in short time of analysis. This novel theoretical and practical approach allows for the extension of the linear range without any further experiments, with several advantages including low reagent consumption, reduced waste generation and time of measurement.
- Published
- 2018
6. Low-cost and reagent-free paper-based device to detect chloride ions in serum and sweat
- Author
-
Danila Moscone, Fabiana Arduini, Claudio Cortese, Renato Massoud, Giuseppe Palleschi, Luca Fiore, Stefano Cinti, Cinti, S, Fiore, L, Massoud, R, Cortese, C, Moscone, D, Palleschi, G, and Arduini, F
- Subjects
Paper ,Serum ,Working electrode ,Cyclic voltammetry ,Silver ,Metal ions in aqueous solution ,Inorganic chemistry ,Analytical chemistry ,02 engineering and technology ,Screen-printed electrodes ,Biosensing Techniques ,01 natural sciences ,Chloride ,Analytical Chemistry ,Ion selective electrode ,Electrochemical cell ,Chlorides ,Limit of Detection ,medicine ,Humans ,Settore CHIM/01 - Chimica Analitica ,Sweat ,Reagent Strips ,Detection limit ,Filter paper-based device ,Chemistry ,010401 analytical chemistry ,Electrochemical sensor ,Electrochemical Techniques ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Electrochemical gas sensor ,Calibration ,0210 nano-technology ,Oxidation-Reduction ,Ion-Selective Electrodes ,medicine.drug - Abstract
The recent goal of sustainability in analytical chemistry has boosted the development of eco-designed analytical tools to deliver fast and cost-effective analysis with low economic and environmental impact. Due to the recent focus in sustainability, we report the use of low-cost filter paper as a sustainable material to print silver electrodes and to load reagents for a reagent-free electrochemical detection of chloride in biological samples, namely serum and sweat. The electrochemical detection of chloride ions was carried out by exploiting the reaction of the analyte (i.e. chloride) with the silver working electrode. During the oxidation wave in cyclic voltammetry the silver ions are produced, thus they react with chloride ions to form AgCl, while in the reduction wave, the following reaction occurs: AgCl + e- -->Ag + Cl-. These reactions at the electrode surface resulted in anodic/cathodic peaks directly proportional to the chloride ions in solution. Chloride ions were detected with the addition of only 10 μL of the sample on the paper-based electrochemical cell, obtaining linearity up to 200 mM with a detection limit equal to 1 mM and relative standard deviation lower than 10%. The accuracy of the sensor was evaluated in serum and sweat samples, with percentage recoveries between 93 ± 10 and 108 ± 8%. Moreover, the results achieved with the paper-based device were positively compared with those obtained by using the gold standard method (Ion Selective Electrode) adopted in routine clinical analyses.
- Published
- 2017
7. Smartphone-assisted electrochemical sensor for reliable detection of tyrosine in serum.
- Author
-
Fiore L, De Lellis B, Mazzaracchio V, Suprun E, Massoud R, Goffredo BM, Moscone D, and Arduini F
- Subjects
- Electrodes, Limit of Detection, Soot, Tyrosine, Electrochemical Techniques, Smartphone
- Abstract
Point-of-care devices have attracted a huge interest by the scientific community because of the valuable potentiality for rapid diagnosis and precision medicine through cost-effective and easy-to-use devices for on-site measurement by unskilled personnel. Herein, we reported a smartphone-assisted electrochemical device consisted of a screen-printed electrode modified with carbon black nanomaterial and a commercially available smartphone potentiostat i.e. EmStat3 Blue, for sensitive detection of tyrosine. Once optimized the conditions, tyrosine was detected in standard solutions by square wave voltammetry, achieving a linear range comprised between 30 and 500 μM, with a detection limit equal to 4.4 μM. To detect tyrosine in serum, the interference of another amino acid i.e. tryptophan was hindered using a sample treatment with an extraction cartridge. The agreement of results analyzing serum samples with HPLC reference method and with the developed smart sensing system demonstrated the suitability of this smartphone-assisted sensing tool for cost-effective and rapid analyses of tyrosine in serum samples., (Copyright © 2021 Elsevier B.V. All rights reserved.)
- Published
- 2022
- Full Text
- View/download PDF
8. An ELIME assay for hepatitis A virus detection.
- Author
-
Micheli L, Fasoli A, Attar A, Donia DT, Divizia M, Amine A, Palleschi G, Salazar Carballo PA, and Moscone D
- Subjects
- Biological Assay, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Sensitivity and Specificity, Hepatitis A virus genetics
- Abstract
An Enzyme Linked ImmunoMagnetic Electrochemical assay (ELIME) was developed for the detection of the hepatitis A virus (HAV). This system is based on the use of new polydopamine-modified magnetic nanobeads as solid support for the immunochemical chain, and an array of 8 screen-printed electrodes as a sensing platform. Enzymatic-by-product is quickly measured by differential pulse voltammetry. For this purpose, all analytical parameters were optimized; in particular, different blocking reagents were evaluated in order to minimize the nonspecific interaction of bioreagents. Using the ELIME assays, a quantitative determination of HAV can be achieved with a detection limit of 1·10
-11 IU mL-1 and a working range between 10-10 - 5 × 10-7 IU mL-1 . The cross-reactivity of the commercial monoclonal antibodies against HAV used in ELIME assays was tested for Coxsackie B4, resulting very low. The sensitivity was also investigated and compared with spectrophotometric sandwich ELISA. The average relative standard deviation (RSD) of the ELIME method was less than 5% for the assays performed on the same day, and 7% for the measurements made on different days. The proposed system was applied to the cell culture of HAV, which title was quantified by Real-Time Quantitative Reverse Transcription PCR (RT¬qPCR). To compare the results, a correlation between the units used in ELIME (IU mL-1 ) and those used in RT¬qPCR (genome mL-1 ) was established using a HAV-positive sample, resulting in 1 IU mL-1 -10-4 gen mL-1 (R2 = 0.978). The ELIME tool exhibits good stability and high biological selectivity for HAV antigen detection and was successfully applied for the determination of HAV in tap water., (Copyright © 2021 Elsevier B.V. All rights reserved.)- Published
- 2021
- Full Text
- View/download PDF
9. Multi-array wax paper-based platform for the pre-concentration and determination of silver ions in drinking water.
- Author
-
Bagheri N, Cinti S, Nobile E, Moscone D, and Arduini F
- Abstract
In this work, a wax-patterned chromatographic paper has been utilized as a holistic platform to 1) synthesize Prussian Blue Nanoparticles (sensing species), 2) load the reagents for the assay, 3) concentrate the sample through multistep, and 4) visualize the determination of silver ions. Waters are continuously affected by changes in the composition, thus the utilization of reagent-free analytical tools is of huge interest for smart drinking water monitoring. Herein, we report the characterization and application of a multi-array paper-based platform for the colorimetric determination of silver ions based on the conversion from Prussian Blue to its silver-based analogue, namely Ag
4 [Fe(CN)6 ]. In particular, the platform highlights the increase of sensitivity due to paper pre-concentration of sample, that can be easily adapted to the analytical necessities. Within the proposed experimental setup, Ag+ is visualized down to a detection limit of 0.9 μM, with high repeatability and satisfactory recoveries in the range comprised between 90 and 113%., (Copyright © 2021 Elsevier B.V. All rights reserved.)- Published
- 2021
- Full Text
- View/download PDF
10. Highly sensitive paper-based electrochemical sensor for reagent free detection of bisphenol A.
- Author
-
Jemmeli D, Marcoccio E, Moscone D, Dridi C, and Arduini F
- Abstract
Bisphenol A is one the most relevant endocrine disruptors for its toxicity and ubiquity in the environment, being largely employed as raw material for manufacturing processes of a wide number of compounds. Furthermore, bisphenol A is released in the drinking water when plastic-based bottles are incorrectly transported under sunlight, delivering contaminated drinking water. For the health of human beings and the environment, rapid and on site detection of bisphenol A in drinking water is an important issue. Herein, we report a novel and cost-effective printed electrochemical sensor for an enzymatic-free bisphenol A detection. This sensor encompasses the entire electrochemical cell printed on filter paper and the reagents for the measurement loaded in the cellulose fiber network, for delivering a reagent-free analytical tool. The working electrode was printed using ink modified with carbon black, a cost effective nanomaterial for sensitive and sustainable bisphenol A determination. Several parameters including pH, frequency, and amplitude were optimized allowing for a detection limit of 0.03 μM with two linear ranges 0.1-0.9 μM and 1 μM-50 μM, using square wave voltammetry as electrochemical technique. The satisfactory recovery values found in river and drinking water samples demonstrated the suitability of this sensor for screening analyses in water samples. These results revealed the attractiveness of this paper-based device thanks to the synergic combination of paper and carbon black as cost-effective materials., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020. Published by Elsevier B.V.)
- Published
- 2020
- Full Text
- View/download PDF
11. Carbon black-based disposable sensor for an on-site detection of free chlorine in swimming pool water.
- Author
-
Tomei MR, Arduini F, Neagu D, and Moscone D
- Abstract
Chlorine is the most common chemical compound used for treatment of water at ppm levels, although at higher concentration it can generate harmful disinfection by-products. To easily monitor the correct level of free chlorine, cost-effective and miniaturized devices for fast and on-site detection are required. Herein, we report the development of a novel miniaturized and cost-effective screen-printed electrode modified with carbon black nanomaterial for an effective amperometric detection of free chlorine in swimming pool water. The carbon black was used as working electrode modifier for chlorine quantification at low applied potential, overcoming the fouling problem. In addition, the use of stable and cost-effective carbon black dispersion allows for a mass-production of the modified sensor by an automatable drop casting method. This sensor has demonstrated the capability to detect free chlorine in a linear range of 0.05-200 ppm with a detection limit of 0.01 ppm in standard solution as well as in swimming pool water. The achieved results demonstrated for the first time the suitability of the carbon black as electrode nanomodifier for free chlorine detection, and the possibility to produce a miniaturized and cost-effective carbon black-based printed sensor for reliable free chlorine quantification in water samples., (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Published
- 2018
- Full Text
- View/download PDF
12. How to extend range linearity in enzyme inhibition-based biosensing assays.
- Author
-
Amine A, Cinti S, Arduini F, Moscone D, and Palleschi G
- Subjects
- Acetylcholinesterase metabolism, Biosensing Techniques economics, Costs and Cost Analysis, Kinetics, Biosensing Techniques methods, Cholinesterase Inhibitors analysis, Enzyme Inhibitors analysis
- Abstract
Bioassays based on enzyme inhibition are analytical tools widely employed for inhibitor analysis. Beside the conventional analytical techniques such as chromatography and mass spectrometry, these bioassays are cost-effective, easy to use, and suitable for in situ measurement but they are often characterised by a quite narrow linear range. Herein, we report a novel graphical method based on integrated Michaelis-Menten equation, valid for all types of reversible inhibition, which provides an extended linear range. The suitability of this innovative approach was demonstrated in the case of fluoride quantification using a colorimetric bioassay based on acetylcholinesterase inhibition. The "half time reaction", estimated by the progress curve of cholinesterase inhibition, was plotted versus the fluoride inhibitor concentration, observing an extended linear range up to 5 mM, instead of 0.6 mM using initial rate measurements. The applicability of this new concept was further demonstrated in the case of catalase enzyme inhibited by cyanide. Furthermore, it was demonstrated that fixed substrate conversion at level of 10-50% allows determination of inhibitor concentration in a wide linear range with high precision and in short time of analysis. This novel theoretical and practical approach allows for the extension of the linear range without any further experiments, with several advantages including low reagent consumption, reduced waste generation and time of measurement., (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Published
- 2018
- Full Text
- View/download PDF
13. Paper-based synthesis of Prussian Blue Nanoparticles for the development of whole blood glucose electrochemical biosensor.
- Author
-
Cinti S, Cusenza R, Moscone D, and Arduini F
- Subjects
- Electrodes, Hydrogen-Ion Concentration, Particle Size, Surface Properties, Biosensing Techniques, Blood Glucose analysis, Electrochemical Techniques, Ferrocyanides chemical synthesis, Ferrocyanides chemistry, Nanoparticles chemistry, Paper
- Abstract
Nowadays, environmentally friendly synthesis pathways for preserving the environment and minimizing waste are strongly required. Herein, we propose filter paper as a convenient scaffold for chemical reactions. To demonstrate this novel approach, Prussian Blue Nanoparticles (PBNPs) were synthesized on filter paper by utilizing few μL of its precursors without external inputs, i.e. pH, voltage, reducing agents, and without producing waste as well. The functional paper, named "Paper Blue", is successfully applied in the sensing field, exploiting the reduction of hydrogen peroxide at low applied potential. The eco-designed "Paper Blue" was combined with wax- and screen-printing to manufacture a reagentless electrochemical point-of-care device for diabetes self-monitoring, by using glucose oxidase as the biological recognition element. Blood glucose was linearly detected for a wide concentration range up to 25 mM (450 mg/dL), demonstrating its suitability for management of diabetes and glucose-related diseases. The Paper Blue-based biosensor demonstrated a correlation coefficient of 0.987 with commercial glucose strips (Bayer Contour XT). The achieved results demonstrated the effectiveness of this approach, which is also extendible to other (bio)systems to be applied in catalysis, remediation, and diagnostics., (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Published
- 2018
- Full Text
- View/download PDF
14. Low-cost and reagent-free paper-based device to detect chloride ions in serum and sweat.
- Author
-
Cinti S, Fiore L, Massoud R, Cortese C, Moscone D, Palleschi G, and Arduini F
- Subjects
- Biosensing Techniques economics, Calibration, Electrochemical Techniques economics, Humans, Ion-Selective Electrodes, Limit of Detection, Oxidation-Reduction, Paper, Reagent Strips economics, Silver chemistry, Biosensing Techniques methods, Chlorides analysis, Electrochemical Techniques methods, Reagent Strips analysis, Serum chemistry, Sweat chemistry
- Abstract
The recent goal of sustainability in analytical chemistry has boosted the development of eco-designed analytical tools to deliver fast and cost-effective analysis with low economic and environmental impact. Due to the recent focus in sustainability, we report the use of low-cost filter paper as a sustainable material to print silver electrodes and to load reagents for a reagent-free electrochemical detection of chloride in biological samples, namely serum and sweat. The electrochemical detection of chloride ions was carried out by exploiting the reaction of the analyte (i.e. chloride) with the silver working electrode. During the oxidation wave in cyclic voltammetry the silver ions are produced, thus they react with chloride ions to form AgCl, while in the reduction wave, the following reaction occurs: AgCl + e
- -->Ag + Cl- . These reactions at the electrode surface resulted in anodic/cathodic peaks directly proportional to the chloride ions in solution. Chloride ions were detected with the addition of only 10μL of the sample on the paper-based electrochemical cell, obtaining linearity up to 200mM with a detection limit equal to 1mM and relative standard deviation lower than 10%. The accuracy of the sensor was evaluated in serum and sweat samples, with percentage recoveries between 93 ± 10 and 108 ± 8%. Moreover, the results achieved with the paper-based device were positively compared with those obtained by using the gold standard method (Ion Selective Electrode) adopted in routine clinical analyses., (Copyright © 2017 Elsevier B.V. All rights reserved.)- Published
- 2018
- Full Text
- View/download PDF
15. Rapid and label-free detection of ochratoxin A and aflatoxin B1 using an optical portable instrument.
- Author
-
Arduini F, Neagu D, Pagliarini V, Scognamiglio V, Leonardis MA, Gatto E, Amine A, Palleschi G, and Moscone D
- Subjects
- Artifacts, Biosensing Techniques, Colorimetry, Fluorometry, Methanol chemistry, Millets chemistry, Optical Fibers, Spectrum Analysis, Time Factors, Wine analysis, Aflatoxin B1 analysis, Food Analysis instrumentation, Food Contamination analysis, Ochratoxins analysis, Optical Devices
- Abstract
In this study, we report a novel assay for the combined on site detection of aflatoxin B1 (AFB1) and ochratoxin A (OTA), through a colorimetric biosensing system for AFB1 and a fluorimetric detection for OTA, exploiting the capability of the portable fibre optic spectrometer to perform both analyses. AFB1 was detected using the acetylcholinesterase (AChE) enzyme that is inhibited by this toxin, and the degree of inhibition was quantified by the Ellman's spectrophotometric method, obtaining a detection limit of 10 µg L(-1). OTA quantification was performed by monitoring its intrinsic fluorescence in methanol, reaching a detection limit of 0.1 µg L(-1). In order to successfully apply the analytical tool in the food analysis, immunoaffinity columns were used. Clean-up and quantification of both AFB1 and OTA in millet samples was obtained by HPLC-dedicated AflaOchra-Test HPLC™ (Vicam™) and Afla-OtaCLEAN™ (LC-Tech) immunoaffinity columns, followed by absorption/fluorescence detection. Millet samples which were fortified with both OTA (50 µg kg(-1)) and AFB1 (20 µg kg(-1)), gave recovery values of 100 ± 6% for OTA, and 110 ± 10% for AFB1, using AflaOchra-Test HPLC™. Single OTA clean-up and quantification in wine samples was obtained, using an OchraTest immunoaffinity column (Vicam™), reaching a detection limit of 0.3 µg L(-1) and recovery values between 80% and 120%. These results demonstrated the possibility of employing a single clean-up and a cost-effective, and easy to use analytical system for both AFB1 and OTA detection at µg kg(-1) (ppb) level. Furthermore, in the case of positive samples, they could be analysed further, using standard chromatographic procedures, without any additional clean-up step, since the same extraction procedure of standard method is proposed in our method., (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Published
- 2016
- Full Text
- View/download PDF
16. Screen-printed electrode modified with carbon black nanoparticles for phosphate detection by measuring the electroactive phosphomolybdate complex.
- Author
-
Talarico D, Arduini F, Amine A, Moscone D, and Palleschi G
- Subjects
- Calibration, Carbon chemistry, Equipment Design, Hydrogen-Ion Concentration, Limit of Detection, Molybdenum chemistry, Phosphoric Acids chemistry, Spectrophotometry methods, Wastewater analysis, Water analysis, Electrochemical Techniques instrumentation, Electrochemical Techniques methods, Electrodes, Nanoparticles chemistry, Phosphates analysis
- Abstract
We report a sensor for phosphate detection based on screen-printed electrodes modified with carbon black nanoparticles. The phosphate was measured in amperometric mode via electrochemical reduction of molybdophosphate complex. Carbon black nanoparticles demonstrated the ability to quantify the molybdophosphate complex at a low applied potential. Some analytical parameters such as the working solution (sulfuric acid 0.1M), applied potential (0.125V vs Ag/AgCl), and molybdate concentration (1mM) were optimized. Using these conditions, a linear range of 0.5-100µM was observed with a detection limit of 0.1µM, calculated as three times the standard deviation of the blank divided by the slope of calibration curve. The system was challenged in drinking, river, aquarium, and waste water samples yielding satisfactory recovery values in accordance with a spectrophotometric reference method which demonstrated the suitability of the screen-printed electrode modified with carbon black nanoparticles coupled with the use of molybdate to detect phosphate in water samples., (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Published
- 2015
- Full Text
- View/download PDF
17. Analytical aspects of enzyme reversible inhibition.
- Author
-
Amine A, El Harrad L, Arduini F, Moscone D, and Palleschi G
- Subjects
- Binding, Competitive, Enzymes, Immobilized metabolism, Kinetics, Monophenol Monooxygenase metabolism, Agaricales enzymology, Antifungal Agents pharmacology, Benzoic Acid pharmacology, Biosensing Techniques methods, Enzymes, Immobilized antagonists & inhibitors, Monophenol Monooxygenase antagonists & inhibitors
- Abstract
A simple graphical method for the determination of reversible inhibition type, inhibition constant (Ki) and estimation of fifty percent of inhibition I₅₀ of an enzyme reaction is described. The method consists of plotting experimental data as "degree of inhibition" versus the inhibitor concentration at two or more concentrations of substrate. Diagnosis of inhibition type is based on determination of and the observation of the shift of the inhibition curves. Relationship between I50 and inhibition constant Ki was discussed. A simplified hyperbolae equation of degree of inhibition showing kinetic orders of 1 and zero at low and high concentrations of inhibitors respectively is proposed. The relative error of inhibitor concentration increased drastically when degree of inhibition reached values of 90%. Examples of published inhibition reports as well as an experimental example of amperometric biosensor based on tyrosinase inhibition by benzoic acid were in agreement with the proposed theoretical approach., (© 2013 Published by Elsevier B.V.)
- Published
- 2014
- Full Text
- View/download PDF
18. Investigation of amperometric detection of phosphate Application in seawater and cyanobacterial biofilm samples.
- Author
-
Quintana JC, Idrissi L, Palleschi G, Albertano P, Amine A, El Rhazi M, and Moscone D
- Abstract
Detection of phosphate using amperometry was investigated. The phosphomolybdate complex, formed by addition of nitric acid, ammonium molybdate and phosphate, was reduced at a carbon paste electrode polarised at +0.3V (versus Ag/AgCl). The major characteristics observed were simplicity of the equipment, a limited consumption of reagents and a low detection limit (0.3mumoll(-1)), with a linear range between 1 and 20mumoll(-1). The interference of silicate was completely eliminated using an appropriate concentration of nitric acid and ammonium molybdate. The amperometric detection of orthophosphate in seawater using the batch injection analysis (BIA) technique was reported. Moreover, a carbon paste microelectrode was constructed. Its use allows the analysis of small volume of samples with little dilution in supporting electrolyte. This method was applied to the determination of orthophosphate in cyanobacterial biofilms collected from Roman catacombs. There was a good statistical correlation between results obtained with the proposed method and the standard spectrophotometric method.
- Published
- 2004
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.