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Present of this study was designed to improve the antioxidant activity of milk thistle (salymarin) against inoculated mice with virulent strain of Corynebacterium Pseudotuberculosis by measuring the serum levels of Free Radicals Peroxinitrite and Malondialdhyde. Fifty mice were divided into five groups equally G1:10 mice served as control negative. G2 served as control positive challenged virulent C. pseudotuberculosis (5x109) CFU/ ml I/P. G3 giving plant extract of salybum marianam 350 mg / kg orally. G4 giving saly marin for thirty days and challenged with Corynebacterium pseudotuberculosis. G5 challenged with virulent C. pseudotuberculosis (5x109) CFU/ ml I/P after thirty days treated with plant extract for thirty days. At thirty days of experiment (5) mice were sacrefecied from each group and serum blood were collected for measuring oxidant levels (Peroxinitrite and Malondialdhyde), also at sixty days of experiment all animal were sacrificed to determine the free radicals level (Peroxinitrite and Malondialdhyde). The results revealed that the serum levels of free radicals in group treated with milk thistle lower than serum levels treated with Corynebacterium psedotuberculosis. This study concludes that the antioxidant activity of milk thistle in prevent and treatment the oxidative stress. [ABSTRACT FROM AUTHOR]

The present study was designed to improve fertility in female mice caused by virulent strain of Brucella melitensis. Sixty female mice were divided into 5 groups equally: Group1 giving normal saline 0.3 ml orally serve as control negative. Group2 served as control positive infected with a virulent strain of B. melitensis(1x10 8) CFU/ ml I/P. Group 3 administrated hesperidin 10mg /kg B.W. group 4 infected with B. melitensisand treated with hesperidin 10mg/kg B.W. Group 5 treated with hesperidin 10mg /kg B.W. and infected with B. melitensis(1x10 8). At thirty and sixty days of the experiment (half and end of the experiment), six mice from each group were sacrificed to determine serum hormones (FSH, LH, estrogen, and progesterone). The results revealed that hesperidin reduces estrogen-progesterone levels and increases levels of FSH and LH in comparison with an infected group (G2) serum levels of estrogen, progesterone was high and FSH and LH were low levels. This study concluded that hesperidin improves fertility because of act as anti-cancer, anti-oxidant anti-inflammatory action that giving protect of female reproductive organs of female mice in comparison with infected mice with B. melitensiswhich caused a different change of hormonal levels that lead to cause infertility. [ABSTRACT FROM AUTHOR]