1. Structure of an estradiol-glutathione conjugate formed in the presence of peroxidase
- Author
-
John S. Elce
- Subjects
Electrophoresis ,Paper ,Magnetic Resonance Spectroscopy ,Chemical Phenomena ,Infrared Rays ,Ultraviolet Rays ,Estranes ,Clinical Biochemistry ,Biochemistry ,Horseradish peroxidase ,Mass Spectrometry ,chemistry.chemical_compound ,Endocrinology ,Secosteroids ,Molecular Biology ,Incubation ,Pharmacology ,Carbon Isotopes ,Estrogens, Conjugated (USP) ,Chromatography ,Estradiol ,biology ,Ion exchange ,Organic Chemistry ,Glutathione ,Nuclear magnetic resonance spectroscopy ,Plants ,Chromatography, Ion Exchange ,Chemistry ,Peroxidases ,chemistry ,Spectrophotometry ,Chromatography, Gel ,biology.protein ,Chromatography, Thin Layer ,Peroxidase ,Conjugate - Abstract
The principal water-soluble compound formed on incubation of estradiol and glutathione in the presence of horseradish peroxidase has been assigned the structure of S-(3,17β-dihydroxy-9,10-secoestra-1,3,5(10)-trien-9-one-10-yl)-glutathione (II). A side product of the incubation was shown to be estra-l,3,5(10),9(11)-tetraene-3,17β-diol. In addition, non-phenolic metabolites were formed in small amounts and were not fully characterized.
- Published
- 1971