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Your search keyword '"Lako, Majlinda"' showing total 9 results
Start Over Author "Lako, Majlinda" Journal stem cells translational medicine
9 results on '"Lako, Majlinda"'

1. pRB-Depleted Pluripotent Stem Cell Retinal Organoids Recapitulate Cell State Transitions of Retinoblastoma Development and Suggest an Important Role for pRB in Retinal Cell Differentiation

Author

Rozanska, Agata, primary, Cerna-Chavez, Rodrigo, additional, Queen, Rachel, additional, Collin, Joseph, additional, Zerti, Darin, additional, Dorgau, Birthe, additional, Beh, Chia Shyan, additional, Davey, Tracey, additional, Coxhead, Jonathan, additional, Hussain, Rafiqul, additional, Al-Aama, Jumana, additional, Steel, David H, additional, Benvenisty, Nissim, additional, Armstrong, Lyle, additional, Parulekar, Manoj, additional, and Lako, Majlinda, additional

Published
2022
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2. Human Retinal Organoids Provide a Suitable Tool for Toxicological Investigations: A Comprehensive Validation Using Drugs and Compounds Affecting the Retina

Author

Dorgau, Birthe, primary, Georgiou, Maria, additional, Chaudhary, Alexander, additional, Moya-Molina, Marina, additional, Collin, Joseph, additional, Queen, Rachel, additional, Hilgen, Gerrit, additional, Davey, Tracey, additional, Hewitt, Philip, additional, Schmitt, Michael, additional, Kustermann, Stefan, additional, Pognan, Francois, additional, Steel, David H, additional, Sernagor, Evelyne, additional, Armstrong, Lyle, additional, and Lako, Majlinda, additional

Published
2022
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3. Systematic Comparison of Retinal Organoid Differentiation from Human Pluripotent Stem Cells Reveals Stage Specific, Cell Line, and Methodological Differences

Author

Mellough, Carla B., Collin, Joseph, Queen, Rachel, Hilgen, Gerrit, Dorgau, Birthe, Zerti, Darin, Felemban, Majed, White, Kathryn, Sernagor, Evelyne, and Lako, Majlinda

Subjects
Adult, lcsh:R5-920, lcsh:Cytology, Cell Culture Techniques, Human embryonic stem cells, Induced pluripotent stem cells, Organoids, Retina, Tissue engineering, Cell Line, Embryoid Bodies, Female, Human Embryonic Stem Cells, Humans, Induced Pluripotent Stem Cells, Cell Differentiation, C400, C700, Tissue Engineering and Regenerative Medicine, lcsh:QH573-671, lcsh:Medicine (General)
Abstract

A major goal in the stem cell field is to generate tissues that can be utilized as a universal tool for in vitro models of development and disease, drug development, or as a resource for patients suffering from disease or injury. Great efforts are being made to differentiate human pluripotent stem cells in vitro toward retinal tissue, which is akin to native human retina in its cytoarchitecture and function, yet the numerous existing retinal induction protocols remain variable in their efficiency and do not routinely produce morphologically or functionally mature photoreceptors. Herein, we determine the impact that the method of embryoid body (EB) formation and maintenance as well as cell line background has on retinal organoid differentiation from human embryonic stem cells and human induced pluripotent stem cells. Our data indicate that cell line‐specific differences dominate the variables that underline the differentiation efficiency in the early stages of differentiation. In contrast, the EB generation method and maintenance conditions determine the later differentiation and maturation of retinal organoids. Of the latter, the mechanical method of EB generation under static conditions, accompanied by media supplementation with Y27632 for the first 48 hours of differentiation, results in the most consistent formation of laminated retinal neuroepithelium containing mature and electrophysiologically responsive photoreceptors. Collectively, our data provide substantive evidence for stage‐specific differences in the ability to give rise to laminated retinae, which is determined by cell line‐specific differences in the early stages of differentiation and EB generation/organoid maintenance methods at later stages.

Published
2019

5. Complement modulation reverses pathology in Y402H-retinal pigment epithelium cell model of age-related macular degeneration by restoring lysosomal function

Author

Cerniauskas, Edvinas, primary, Kurzawa-Akanbi, Marzena, additional, Xie, Long, additional, Hallam, Dean, additional, Moya-Molina, Marina, additional, White, Kathryn, additional, Steel, David, additional, Doherty, Mary, additional, Whitfield, Phil, additional, Al-Aama, Jumana, additional, Armstrong, Lyle, additional, Kavanagh, David, additional, Lambris, John D., additional, Korolchuk, Viktor I., additional, Harris, Claire, additional, and Lako, Majlinda, additional

Published
2020
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7. In the eye of the storm: SARS‐CoV‐2infection and replication at the ocular surface?

Author

Armstrong, Lyle, Collin, Joseph, Mostafa, Islam, Queen, Rachel, Figueiredo, Francisco C., and Lako, Majlinda

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) first emerged in December 2019 and spread quickly causing the coronavirus disease 2019 (COVID‐19) pandemic. Recent single cell RNA‐Seq analyses have shown the presence of SARS‐CoV‐2 entry factors in the human corneal, limbal, and conjunctival superficial epithelium, leading to suggestions that the human ocular surface may serve as an additional entry gateway and infection hub for SARS‐CoV‐2. In this article, we review the ocular clinical presentations of COVID‐19 and the features of the ocular surface that may underline the overall low ocular SARS‐CoV‐2 infection. We critically evaluate the studies performed in nonhuman primates, ex vivo organ culture ocular models, stem cell derived eye organoids and the differences in infection efficiency observed in different parts of human ocular surface epithelium. Finally, we highlight the additional work that needs to be carried out to understand the immune response of the ocular surface to SARS‐CoV‐2 infection, which can be translated into prophylactic treatments that may be applied to other organ systems. Schematic showing potential SARS‐CoV‐2 entry into the eye via infection of ocular surface epithelium (left hand side panel) or the systemic routes (right hand side panel).

Published
2021
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8. An Induced Pluripotent Stem Cell Model of Hypoplastic Left Heart Syndrome (HLHS) Reveals Multiple Expression and Functional Differences in HLHS-Derived Cardiac Myocytes

Author

Jiang, Yan, primary, Habibollah, Saba, additional, Tilgner, Katarzyna, additional, Collin, Joseph, additional, Barta, Tomas, additional, Al-Aama, Jumana Yousuf, additional, Tesarov, Lenka, additional, Hussain, Rafiqul, additional, Trafford, Andrew W., additional, Kirkwood, Graham, additional, Sernagor, Evelyne, additional, Eleftheriou, Cyril G., additional, Przyborski, Stefan, additional, Stojković, Miodrag, additional, Lako, Majlinda, additional, Keavney, Bernard, additional, and Armstrong, Lyle, additional

Published
2014
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9. An Induced Pluripotent Stem Cell Model of Hypoplastic Left Heart Syndrome (HLHS) Reveals Multiple Expression and Functional Differences in HLHS-Derived Cardiac Myocytes.

Author

YAN JIANG, HABIBOLLAH, SABA, TILGNER, KATARZYNA, COLLIN, JOSEPH, BARTA, TOMAS, AL-AAMA, JUMANA YOUSUF, TESAROV, LENKA, HUSSAIN, RAFIQUL, TRAFFORD, ANDREW W., KIRKWOOD, GRAHAM, SERNAGOR, EVELYNE, ELEFTHERIOU, CYRIL G., PRZYBORSKI, STEFAN, STOJKOVIĆ, MIODRAG, LAKO, MAJLINDA, KEAVNEY, BERNARD, and ARMSTRONG, LYLE

Published
2014
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