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Start Over Author "Di Noto R" Journal stem cells and development
3 results on '"Di Noto R"'

1. High aminopeptidase N/CD13 levels characterize human amniotic mesenchymal stem cells and drive their increased adipogenic potential in obese women

Author

Maddalena Raia, Maddalena Ferrigno, Lucio Pastore, Laura Iaffaldano, Elisabetta Mariotti, Giuseppe Maria Maruotti, F. Quaglia, Pasquale Martinelli, Luigi Del Vecchio, Lucia Sacchetti, Rosa Di Noto, Giuseppe Labruna, Valentina Capobianco, Angela Capone, Carmela Nardelli, Iaffaldano, Laura, Nardelli, Carmela, Raia, M, Mariotti, E, Ferrigno, M, Quaglia, F, Labruna, G, Capobianco, Valentina, Capone, A, Maruotti, Gm, Pastore, Lucio, Di Noto, R, Martinelli, Pasquale, Sacchetti, L, and DEL VECCHIO, Luigi

Subjects
Adult, medicine.medical_specialty, Adipose tissue, Gene Expression, Biology, CD13 Antigens, Body Mass Index, Immunophenotyping, Antigen, Original Research Reports, Pregnancy, Risk Factors, Internal medicine, medicine, Humans, Amnion, Obesity, RNA, Messenger, RNA, Small Interfering, Fetus, Adipogenesis, Mesenchymal stem cell, Infant, Newborn, Mesenchymal Stem Cells, Cell Biology, Hematology, medicine.disease, PPAR gamma, Endocrinology, In utero, Case-Control Studies, Female, Body mass index, Developmental Biology
Abstract

Maternal obesity is associated to increased fetal risk of obesity and other metabolic diseases. Human amniotic mesenchymal stem cells (hA-MSCs) have not been characterized in obese women. The aim of this study was to isolate and compare hA-MSC immunophenotypes from obese (Ob-) and normal weight control (Co-) women, to identify alterations possibly predisposing the fetus to obesity. We enrolled 16 Ob- and 7 Co-women at delivery (mean/SEM prepregnancy body mass index: 40.3/1.8 and 22.4/1.0 kg/m2, respectively), and 32 not pregnant women. hA-MSCs were phenotyped by flow cytometry; several maternal and newborn clinical and biochemical parameters were also measured. The expression of membrane antigen CD13 was higher on Ob-hA-MSCs than on Co-hA-MSCs (P = 0.005). Also, serum levels of CD13 at delivery were higher in Ob- versus Co-pregnant women and correlated with CD13 antigen expression on Ob-hA-MSCs (r2 = 0.84, P < 0.0001). Adipogenesis induction experiments revealed that Ob-hA-MSCs had a higher adipogenic potential than Co-hA-MSCs as witnessed by higher peroxisome proliferator-activated receptor gamma and aP2 mRNA levels (P = 0.05 and P = 0.05, respectively), at postinduction day 14 associated with increased CD13 mRNA levels from baseline to day 4 postinduction (P < 0.05). Adipogenesis was similar in the two sets of hA-MSCs after CD13 silencing, whereas it was increased in Co-hA-MSCs after CD13 overexpression. CD13 expression was high also in Ob-h-MSCs from umbilical cords or visceral adipose tissue of not pregnant women. In conclusion, antigen CD13, by influencing the adipogenic potential of hA-MSCs, could be an in utero risk factor for obesity. Our data strengthen the hypothesis that high levels of serum and MSC CD13 are obesity markers.

Published
2013

2. High aminopeptidase N/CD13 levels characterize human amniotic mesenchymal stem cells and drive their increased adipogenic potential in obese women.

Author

Iaffaldano L, Nardelli C, Raia M, Mariotti E, Ferrigno M, Quaglia F, Labruna G, Capobianco V, Capone A, Maruotti GM, Pastore L, Di Noto R, Martinelli P, Sacchetti L, and Del Vecchio L

Subjects
Adult, Amnion growth & development, Amnion pathology, Body Mass Index, CD13 Antigens antagonists & inhibitors, CD13 Antigens genetics, Case-Control Studies, Female, Gene Expression, Humans, Immunophenotyping, Infant, Newborn, Mesenchymal Stem Cells cytology, Obesity enzymology, Obesity pathology, PPAR gamma genetics, PPAR gamma metabolism, Pregnancy, RNA, Messenger antagonists & inhibitors, RNA, Messenger genetics, RNA, Small Interfering genetics, RNA, Small Interfering metabolism, Risk Factors, Adipogenesis genetics, Amnion enzymology, CD13 Antigens metabolism, Mesenchymal Stem Cells enzymology, Obesity genetics, RNA, Messenger metabolism
Abstract

Maternal obesity is associated to increased fetal risk of obesity and other metabolic diseases. Human amniotic mesenchymal stem cells (hA-MSCs) have not been characterized in obese women. The aim of this study was to isolate and compare hA-MSC immunophenotypes from obese (Ob-) and normal weight control (Co-) women, to identify alterations possibly predisposing the fetus to obesity. We enrolled 16 Ob- and 7 Co-women at delivery (mean/SEM prepregnancy body mass index: 40.3/1.8 and 22.4/1.0 kg/m2, respectively), and 32 not pregnant women. hA-MSCs were phenotyped by flow cytometry; several maternal and newborn clinical and biochemical parameters were also measured. The expression of membrane antigen CD13 was higher on Ob-hA-MSCs than on Co-hA-MSCs (P = 0.005). Also, serum levels of CD13 at delivery were higher in Ob- versus Co-pregnant women and correlated with CD13 antigen expression on Ob-hA-MSCs (r2 = 0.84, P < 0.0001). Adipogenesis induction experiments revealed that Ob-hA-MSCs had a higher adipogenic potential than Co-hA-MSCs as witnessed by higher peroxisome proliferator-activated receptor gamma and aP2 mRNA levels (P = 0.05 and P = 0.05, respectively), at postinduction day 14 associated with increased CD13 mRNA levels from baseline to day 4 postinduction (P < 0.05). Adipogenesis was similar in the two sets of hA-MSCs after CD13 silencing, whereas it was increased in Co-hA-MSCs after CD13 overexpression. CD13 expression was high also in Ob-h-MSCs from umbilical cords or visceral adipose tissue of not pregnant women. In conclusion, antigen CD13, by influencing the adipogenic potential of hA-MSCs, could be an in utero risk factor for obesity. Our data strengthen the hypothesis that high levels of serum and MSC CD13 are obesity markers.

Published
2013
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3. Comparative characteristics of mesenchymal stem cells from human bone marrow and placenta: CD10, CD49d, and CD56 make a difference.

Author

Mariotti E, Mirabelli P, Abate G, Schiattarella M, Martinelli P, Fortunato G, Di Noto R, and Del Vecchio L

Subjects
Adolescent, Adult, Bone Marrow Cells enzymology, Cell Differentiation drug effects, Cell Membrane drug effects, Cell Membrane metabolism, Cell Movement drug effects, Chemokine CXCL12 pharmacology, Endothelium drug effects, Endothelium metabolism, Female, Flow Cytometry, Fucose metabolism, Gene Expression Regulation drug effects, Glycosyltransferases metabolism, Humans, Intracellular Space drug effects, Intracellular Space metabolism, Mesenchymal Stem Cells drug effects, Mesenchymal Stem Cells enzymology, Multipotent Stem Cells drug effects, Multipotent Stem Cells enzymology, Phenotype, Placenta enzymology, RNA, Messenger genetics, RNA, Messenger metabolism, Receptors, Chemokine genetics, Receptors, Chemokine metabolism, Selectins metabolism, Vascular Cell Adhesion Molecule-1 metabolism, Bone Marrow Cells cytology, CD56 Antigen, Integrin alpha4, Mesenchymal Stem Cells cytology, Multipotent Stem Cells cytology, Neprilysin, Placenta cytology
Published
2008
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