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5 results on '"Tabilio, Antonio"'

1. Transformation by Retroviral Vectors of Bone Marrow-Derived Mesenchymal Cells Induces Mitochondria-Dependent cAMP-Sensitive Reactive Oxygen Species Production

Author

Piccoli, Claudia, primary, Scrima, Rosella, additional, Ripoli, Maria, additional, Di Ianni, Mauro, additional, Del Papa, Beatrice, additional, D'Aprile, Annamaria, additional, Quarato, Giovanni, additional, Martelli, Maria Paola, additional, Servillo, Giuseppe, additional, Ligas, Claudio, additional, Boffoli, Domenico, additional, Tabilio, Antonio, additional, and Capitanio, Nazzareno, additional

Published
2008
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2. Sustained Long-Term Engraftment and Transgene Expression of Peripheral Blood CD34+Cells Transduced with Third-Generation Lentiviral Vectors

Author

Tesio, Melania, primary, Gammaitoni, Loretta, additional, Gunetti, Monica, additional, Leuci, Valeria, additional, Pignochino, Ymera, additional, Jordaney, Noela, additional, Capellero, Sonia, additional, Cammarata, Cristina, additional, Caione, Luisa, additional, Migliaretti, Giuseppe, additional, Fagioli, Franca, additional, Tabilio, Antonio, additional, Aglietta, Massimo, additional, and Piacibello, Wanda, additional

Published
2008
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4. In Vivo Demethylation of a MoMuLV Retroviral Vector Expressing the Herpes Simplex Thymidine Kinase Suicide Gene by 5′ Azacytidine

Author

Di Ianni, Mauro, primary, Terenzi, Adelmo, additional, Di Florio, Sabrina, additional, Venditti, Gigliola, additional, Benedetti, Roberta, additional, Santucci, Antonella, additional, Bartoli, Andrea, additional, Fettucciari, Katia, additional, Marconi, Pierfrancesco, additional, Rossi, Ruggero, additional, Martelli, Massimo F., additional, and Tabilio, Antonio, additional

Published
2000
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5. Sustained Long-Term Engraftment and Transgene Expression of Peripheral Blood CD34+ Cells Transduced with Third-Generation Lentiviral Vectors.

Author

Tesio, Melania, Gammaitoni, Loretta, Gunetti, Monica, Leuci, Valeria, Pignochino, Ymera, Jordaney, Noela, Capellero, Sonia, Cammarata, Cristina, Caione, Luisa, Migliaretti, Giuseppe, Fagioli, Franca, Tabilio, Antonio, Aglietta, Massimo, and Piacibello, Wanda

Subjects
TRANSGENE expression, LENTIVIRUS diseases, HEMATOPOIETIC stem cells, GREEN fluorescent protein, STEM cells, BLOOD cells, BACTERIOPHAGES, GENETIC engineering
Abstract

As mobilized peripheral blood (MPB) represents an attractive cell source for gene therapy, we investigated the ability of third-generation lentiviral vectors (LVs) to transfer the enhanced green fluorescent protein gene into MPB CD34+ cells in culture conditions allowing expansion of transplantable human hematopoietic stem cells. To date, few studies have reported transduction of MPB cells with vesicular stomatitis virus G pseudotyped LVs. The critical issue remains whether primitive, hematopoietic repopulating cells have, indeed, been transduced. In vitro (5 weeks' culture in FLT3 ligand + thrombopoietin + stem cell factor + interleukin 6) and in vivo (serial transplantation in NOD/SCID mice) experiments show that MPB CD34+ cells can be effectively long-term transduced by LV and maintain their proliferation, self-renewal, and multilineage differentiation potentials. We show that expansion following transduction improves the engraftment of transduced MPB CD34+ (4.6-fold expansion of SCID repopulating cells by limiting dilution studies). We propose ex vivo expansion after transduction as an effective tool to improve gene therapy protocols with MPB. [ABSTRACT FROM AUTHOR]

Published
2008
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