Search

Your search keyword '"Tavassoli, Marjan"' showing total 2 results
Start Over Author "Tavassoli, Marjan" Journal star protocols
2 results on '"Tavassoli, Marjan"'

1. An efficient and cost-effective purification protocol for Staphylococcus aureus Cas9 nuclease

Author

Teng, Allen C.T., primary, Tavassoli, Marjan, additional, Shrestha, Suja, additional, Marks, Ryan M., additional, McFadden, Meghan J., additional, Evagelou, Sonia L., additional, Lindsay, Kyle, additional, Vandenbelt, Ava, additional, Li, Wenping, additional, Ivakine, Evgueni, additional, Cohn, Ronald, additional, Santerre, J. Paul, additional, and Gramolini, Anthony O., additional

Published
2023
Full Text
View/download PDF

2. An efficient and cost-effective purification protocol for Staphylococcus aureusCas9 nuclease

Author

Teng, Allen C.T., Tavassoli, Marjan, Shrestha, Suja, Marks, Ryan M., McFadden, Meghan J., Evagelou, Sonia L., Lindsay, Kyle, Vandenbelt, Ava, Li, Wenping, Ivakine, Evgueni, Cohn, Ronald, Santerre, J. Paul, and Gramolini, Anthony O.

Abstract

Here, we describe a protocol for purifying functional clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (Cas9) from Staphylococcus aureuswithin 24 h and over 90% purity. SaCas9 purification begins with immobilized metal affinity chromatography, followed by cation exchange chromatography, and ended with centrifugal concentrators. The simplicity, cost-effectiveness, and reproducibility of such protocols will enable general labs to produce a sizable amount of Cas9 proteins, further accelerating CRISPR research.

Published
2022
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results