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The determination of pollution status is an important process of environmental quality monitoring especially in strategic waters for coastal areas, such as in Lampung Bay. An effective and sensitive Water Quality Index (WQI) method is needed, to accurately determine the environmental pollution status. This study aimed to compare the sensitivity of Storage and Retrieval of Water Quality data System (STORET) and Pollution Index (PI) as a WQI method, a case study from Lampung Bay coastal waters, Indonesia. Water quality analysis i.e. Dissolve Oxygen (DO), pH, salinity, nitrite, nitrate, phosphate and ammonia was conducted spatially (three zones of Lampung Bay; river mouth, aquaculture and bay area) and seasonally (April and October 2015). The study found that nitrate and phosphate values were exceeded the limits of water quality standard (Indonesia Ministry of Environment Decree No. 51/2004) for marine organisms. However it may still support the aquaculture activities. The two WQI methods produced different pollution status of Lampung bay. STORET was found to be more sensitive method. Pollution Index method revealed a status of moderately polluted while STORET showed heavily polluted status. Therefore, this study suggest the use of STORET index, compare to Pollution Index, in an assessment of pollution status at watershed area.

Previously designed endpoint PCR has been adapted for use with real time PCR to detect the presence of Salmonella typhimurium and Salmonella enteritidis in fish. Optimization of a standard curve in the presence of herring sperm DNA as background matrix indicated that the real time PCR highly efficient with the Pearson coefficient of determination (R2) value = 0.99937 and slope (M) value = -3.44. An enrichment method (overnight culture) significantly increased (p