1. Multiphoton imaging to distinguish grana and starch inside an intact leaf
- Author
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Po-Fu Chen, Tzu-Ming Liu, Guan Yu Zhuo, Shi-Wei Chu, Pei-Chun Wu, and Mei-Yu Chen
- Subjects
Chloroplast ,chemistry.chemical_compound ,Multiphoton fluorescence microscope ,chemistry ,Starch ,Chlorophyll ,Biophysics ,food and beverages ,Photosynthesis ,Two photon fluorescence ,Multiphoton imaging - Abstract
We have demonstrated a straightforward and noninvasive method to identify the distribution of grana and starch within an intact leaf. Grana and starch are the major functional structures for photosynthesis and energy storage of plant, respectively. Both exhibit highly ordered molecular structures and appear as micrometer-sized granules inside chloroplasts. In order to distinguish grana and starch, we used multiphoton microscopy, with simultaneous acquisition of two photon fluorescence (2PF) and second harmonic generation (SHG) signals. Consequently, SHG is found on both grana and starch while 2PF from chlorophyll indicates the identity of grana.
- Published
- 2013