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A selective, new, rapid and nondestructive Fourier transform Infrared spectroscopic assay has been developed for simultaneous determination of Memantine hydrochloride and Amisulpride in human plasma and their pharmaceutical formulations without interference from common dugs excipients. A binary mixture of ME and nonselective β-blocker namely; carvidalol has been determined the solid-state by FTIR spectroscopy for the first time. The linear range had been extent from 1.0 to 8.0 and 1.0 to 10.0 μg/mg, for ME and AMS respectively. The detection limits were 0.29 and 0.23 μg/mg while quantitation limits were 0.90 and 0.71 μg/mg for ME and AMS respectively. The developed assay has been validated according to ICH & USP recommendations and successfully applied for quantitative determination of selected drugs in biological fluid., (Copyright © 2020 Elsevier B.V. All rights reserved.)

Accurate, simple, sensitive, and fast spectrophotometric assay was applied for the quantification of certain anti-Alzheimer drug namely; Memantine hydrochloride, in its bulk and pharmaceutical preparation. The described assay has been established on the reaction between the primary amino moiety of the cited drug and 2,2-dihydroxyindane-1,3-dione reagent in N,N'-dimethylformamide medium in boiling water bath. Which give Ruhemann's purple color that can be determined at λ max  = 595 nm. Beer's law has been obeyed within drug concentration range from 10-120 μg per milliliter. Detection limit and quantitation limit have been 1.6 & 4.9 μg per milliliter respectively. Developed procedure has been validated in agreement with International Conference of Horizon recommendations and applied successfully for detection of cited drug in bulk, pharmaceutical dosage form and content uniformity testing., (Copyright © 2019 Elsevier B.V. All rights reserved.)

A new, selective and accurate spectrofluorimetric assay has been described for detection of Amisulpride and Memantine hydrochloride in pharmaceutical formulations and real plasma samples. The described assay depends on the reaction between the primary amino group of the selected drugs with acetyl acetone & formaldehyde in an acetate buffer of pH4.8. The derivatized product showed yellow fluorescence at λ ex =418nm and λ em =484.5nm. The calibration graph was linear in the range of 0.05-0.5 and 0.2-1μgmL -1 for AMS and ME, orderly. The limits of detection were 0.0085 and 0.0153μgmL -1 , and the limits of quantitation were 0.026 and 0.0464μgmL -1 for AMS and ME respectively. Validation of the described assay was in consonance with ICH guideline. Due to the sensitivity of the prescribed assay, it permits the determination of selected medications in biological sample quantitatively., (Copyright © 2019 Elsevier B.V. All rights reserved.)