1. Coumarin/fluorescein-fused fluorescent dyes for rapidly monitoring mitochondrial pH changes in living cells.
- Author
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Qi, Sujie, Li, Qi, Liu, Weimin, Ren, Haohui, Zhang, Hongyan, Wu, Jiasheng, Ge, Jiechao, and Wang, Pengfei
- Subjects
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COUMARINS , *BIOLUMINESCENCE , *PHOTOSYNTHESIS , *FLUORESCENCE , *FLUORINE - Abstract
On base of the good optical properties of coumarin and fluorescein, we designed and synthesized two coumarin/fluorescein-fused fluorescent dyes ( CF dyes), which enlarged the emission wavelength and increased the Stokes shift of fluorescein moiety. The corresponding optical properties of CF dyes were investigated in detail. CF dyes could easily introduce other groups to design different functional molecules. CF dyes also exhibited rapid and sensitive responses to pH values in the range of 4.0–7.4 through the characterization of absorption and fluorescence spectra in buffer solution. More importantly, CF ethyl ester dye ( CFE dye) not only showed good cell membrane permeability and low cytotoxicity, but also had the ability to rapidly monitor mitochondrial pH changes in living cells. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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