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【Objective】 To provide scientific reference for the investigation of heavy metal toxicity on Chinese herbal medicine, the effects of Cd pollution on the variation of plant physiological and biochemical characteristics of Duchesnea indica (Andr. ) Focke were studied. [Method】 The simulated experiments of Cd contamination were carried out in pots which was used for planting Duchesnea indica (Andr. ) Focke. After a certain period of growth, the stable seedlings were irrigated by Cd2 + solution of different concentration (11, 22, 45,90 and 134 mg/ kg) with 50 mL/d, and their growth status of5,30,45 days were recorded. Meanwhile, the contents of total soluble sugar, total flavonoids, and antioxidant enzyme activity of the plant were determined. [ Result] It showed that with increasing Cd pollution in the soil, Cd content in Duchesnea indica (Andr.) Focke was kept rising. In the meantime, the total soluble sugar, total flavonoids and the activity of antioxidant enzymes in leaf, stem and root showed a trend of decrease.【Conclusion】] Cd could be easily accumulated in Duchesnea indica ( Andr.) Focke and damaged the metabolism and normal growth of plant, leading to the decrease of physiological and biochemical characteristic indexes ,showing a harmful effect on the plant. [ABSTRACT FROM AUTHOR]

The present paper is to clone a trypsin inhibitor gene from Cassia oblusifolia (0OTI2) and study its expression under various stresses. Tlie partial cDNA, including the complete 3-terminal cDNA of the COTI2 gene was isolated by RACE technique. The expression under various stresses was studied with real time-PCR. The result showed that COTI2 gene contained 565 bp and contained typical domain of Kunitz inhibitors. The PI residue of C0TI2 was composed of Ser38. Multiple alignment analysis based on amino acids of Kunitz inhibitor genes from various plants indicated that C0T12 had the highest similarity with Popidus Irichocarpa. There were the expression patterns of GOTO under various stresses. The research would provide a !>asis for further study on genetic function and regulatory mechanism of C0TI2. [ABSTRACT FROM AUTHOR]

Mature leaves of diploid, tetraploid and hexaploid of Camellia reticulate( Theaceae) were produced by paraffin method. The anatomical characters of drought related including thickness of the cutin, thickness of the upper epidermis, thickness of the lower epidermis, leaves thickness, thickness of palisade tissue, thickness of spongy tissue, ratio between palisade tissue to spongy tissue, tightness of leaves palisade tissue structure and looseness of leaves sponge tissue structure were investigated. They were measured by optical microscopy and analyzed statistically. The results showed that all of the indices were significantly or highly significantly different. At last 5 anatomical structure indices with most representativeness were selected. Comprehensive analysis using subordinative function method indicated that the potential of drought resistance in tetraploid was the largest, followed by diploid and hexaploid. The result would be used to the further study of Camellia reticulate in directed breeding. [ABSTRACT FROM AUTHOR]