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[Objective] The study aimed to investigate the expression and localization of FKBP5 gene and protein in different tissues and different stages of follicular development in Mianalatai sheep. [Method] The full-length CDS region of FKBP5 gene in Altay sheep was amplified by PCR, and a molecular phylogenetic tree was constructed; The expression levels of FKBP5 gene and protein in different organs and tissues such as ovary of Altay sheep were detected by real-time qPCR and Western blot; The expression of FKBP5 gene in follicles of different sizes and corpus luteum of Altay sheep was detected by real-time qPCR; And the expression localization of FKBP5 protein in ovary of Altay sheep was detected by immuno histochemical technique. [Result] The CDS region sequence of FKBP5 gene in Altay sheep was 1390 bp in length and encoded 462 amino acids; sheep was closely related to goats and cattle and distantly related to chickens (birds) ; FKBP5 gene and protein were expressed in sheep heart, liver, spleen, lung, kidney, uterus, ovary, fallopian tube and testis, with the highest expression in the fallopian tube, followed by liver, spleen, uterus, ovary and testis organs and tissues, with lower expression in the heart, lung and kidney; FKBP5 gene expression was the highest in large follicles and extremely significantly higher than that in ovaries, small follicles and corpus luteum (P＜0.01), followed by ovaries and small follicles, with the lowest expression in the corpus luteum; FKBP5 gene expression was slightly different in different cells of large and small follicles.Its expression in large theca cells was significantly higher than that in other types of cells (P＜0.01), while its expression in large theca GCs was also significantly higher than that in small theca GCs, theca cells, and cumulus cells of large and small follicles (P＜0.05) ; FKBP5 protein was expressed in primary follicles, secondary follicles, corpus luteum tissues, and oocytes, cumulus cells, granulosa cells, and theca cells of large and small antral follicles in Altay sheep. [Conclusion] It is speculated that FKBP5 gene may play a role in follicular development in Altay sheep. [ABSTRACT FROM AUTHOR]

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【Objective】The present paper was conducted to screen differentially expressed miRNA from lambs and adult ewes through modern molecular biology and high-throughput sequencing technology,and further dig out and identify related genes related to follicular development,and reveal signaling pathways and molecular regulatory networks of their differences in traits. 【Method】With lamb and ewe granulosa cells as the research object,the high-throughput sequencing(RNA-Seq) technology for RNA library construction was applied to reveal the genetic differences between the lamb and adult sheep from the transcriptome level,the identification of differentially expressed miRNA,and the differences of expression of MI RNA and its target gene functional annotation and clustering analysis and pathway enrichment etc were studied.【Result】More than 200 miRNAs were identified from two miRNA libraries,and 1/3 of them were identified as new miRNAs. Of the9 differences in the common identification of miRNA,the relative ewes,the lamb had 4 miRNA up-regulated and 5 down-regulation. Among them,the up regulation of oar-miR-106 a was the most significant(1. 85),and the oar-miR-10 a downregulation was the most significant(-2. 20). The GO enrichment and KEGG pathway analysis of all the target genes of all miRNAs in two libraries showed that the abundance of PI3 K/Akt signaling pathway was the highest. Real time fluorescence quantitative PCR confirmed 5 randomly selected miRNA,and the results were consistent with the sequencing data. 【Conclusion】miRNAs may play an important role in the reproductive physiology of sheep after superovulation,including the development of follicle and oocyte and the proliferation of granulosa cells. [ABSTRACT FROM AUTHOR]

【Objective】 Platelet glycoprotein 4(CD36) gene plays a variety of biological functions in model animals, which may play an important role in the development of sheep follicles by transcriptome analysis, and the sequence structure and biological function of TSP-1 gene were studied. 【Method】The full-length TSP-1 gene of Aletai sheep CDS region amplified by PCR software was obtained, the protein sequences and functional region of the CD36 gene were analyzed, and while using the method of fluorescence quantitative PCR, the expression of 5 heads of 1 years of Aletai sheep in 10 organizations were detected. 【Result】The CD36 gene was 1419 bp in length, encoding 472 amino acids, which was in full accord with the predicted sequence. Phylogenetic tree analysis showed that the genetic distance between sheep and cattle was close. CD36 protein is a lipophilic hydrophilic protein with 1 transmembrane helix structure, amino acids in the 1-20 position in the signal peptide, 8 N-glycosylation and 41 potential phosphorylation sites, including 19 Ser, 15 Thr and 7 Tyr. Detection of CD36 gene in different tissues and different diameters of follicles showed that CD36 gene expressed in 7 tissues of sheep, heart and lung and small follicles in the expression of CD36 was significantly different(P < 0.05), and the overall expression level in the different diameters of follicles was low. 【Conclusion】 The results showed that CD36 gene may play an important role in the development of sheep follicles. [ABSTRACT FROM AUTHOR]

Copyright of Southwest China Journal of Agricultural Sciences is the property of Editorial Department of Southwest China Journal of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)