Search

[Objective] The present paper aimed to analyze the diversity of bacterial communities in the rhizosphere soil of adzuki bean varieties with different resistance to soybean cyst nematode ( SCN) race 3 and explore the correlation between the composition of rhizosphere bacterial communities and variety resistance. [ Method] In July 2021, the 16S rDNA genome of bacteria in the rhizosphere soil of four different resistant adzuki bean varieties under the stress of SCN race 3 was sequenced by Illumina Miseq high-throughput sequencing technology from the rhizosphere soil of four different resistant adzuki bean varieties in Heihe city, Heilongjiang province, to analyze the changes of bacterial community structure. [ Result] A total of 1959 OTU s were obtained from 4 soil samples, and 30 phyla, 85 classes, 192 orders, 296 families, 481 genera and 893 species of bacteria were identified, among which Actinobacteriota, Proteobacteria, Acidobacteriota, Chloroflexi and Gemmatimonadota were the dominant phyla, Sphingomonas, norankJ __ norank_o __ Gaiellales, norankJ __ norank_o __ Acidobacteriales, Gemmatimonas, Blastococcus were the dominant genus. The average relative abundance of Actinobacteriota, Proteobacteria and Gemmatimonadota in the rhizosphere soil of susceptible varieties was lower than that of resistant varieties. The relative abundance of Gemmatimonas and Sphingomonas, which were related to disease resistance, was high in the rhizosphere soil samples of resistant variety Liaohong 1 and immunevariety Jihong 9218. [ Conclusion] The bacterial community structure in the rhizosphere soil of different resistant adzuki beans changed under the stress of SCN race 3, and the relative abundance of antagonistic bacterial populations in the rhizosphere soil of resistant varieties was much higher, which indicated that the bacterial community structure in the rhizosphere soil may be correlated with the resistance of adzuki beans to SCN. [ABSTRACT FROM AUTHOR]