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[Objective] The present paper aimed to clear the effects of different biological agents on tomato growth. [Method] This study designed field split plot experiment and adopted biometrical method to research the effects of Bacillus subtilis agent(30 kg·hm-2), Trichoderma humatum agent(22.5 kg·hm-2), Yufeng ‘99’agent(30 kg·hm-2), Zhongnonglyukang agent(45 kg·hm-2) on tomato growth, yield, quality and soil physico-chemical properties in 2017-2018, and the application effects were compared and analyzed with the conventional chemical fertilizer(2179.5 kg·hm-2) simultaneously. [Result] There were obvious promotion effects on tomato growth, yield and quality when four biological agents applied. The tomato height increased by 10-26 cm and the stem diameter increased by 0.6-3.0 cm compared with the control. The fruit yield was increased significantly under different biological agents application, which was 1.22-1.53 times higher than that of the control and was 34.33 %-53.65% higher than that of the chemical fertilizer treatment.Besides, the incidence rate of umbilical rot and gray mold diseases of tomato were significantly reduced. The external and internal quality of tomato were improved in each agent application treatment, comparing with the chemical fertilizer treatment, the fruit color uniformity and maturity were increased significantly, and comparing with the control, the fruit Vc content increased by 14.40%-49.94 %, the sugar content increased by 8.68 %-37.85 %, and the titratable acidity reduced by 28.89%-56.76%.Meanwhile, these biological agents application through drip irrigation had significant effect on physico-chemical properties, compared with the chemical fertilizer treatment, the organic matter content increased by 30.43 %-83.48 %, and the soil pH and conductivity decreased slightly, which reduced the occurrence of soil salinization, and compared with the control treatment, the organic matter content increased by 66.67%-134.44 %, and the available nitrogen, phosphorus and potassium also increased significantly. These data showed that the four biological agents could improve soil fertility. [Conclusion] This stuly indicated that the four biological agents could promote tomato growth, reduce diseases and improve yield and quality, the order of the promotion effects was as follows:yufeng‘99’agent> Zhongnonglyukang agent> Bacillus subtilis agent> Trichoderma humatum agent, which was closely related to component and mechanism of different biological agents and the effects of agents on soil microenvironment. [ABSTRACT FROM AUTHOR]