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[Objective] The present paper further explored the interaction between genotype and environment of different barley varieties, and provided theory and method for high quality cultivation of forage barley in different regions. [Method] The average value and significance level of yield traits of different varieties of forage barley at different altitudes were analyzed. The average value of yield traits in different regions were calculated and analyzed by multiple comparisons. The genetic variance, environmental variance and genetic environment interaction variance were estimated to compare the effects of heredity and environment on yield traits of feed barley. [Result] There were significant differences in effective panicle, number of filled grains, 1000 grain weight and yield among barley varieties; The results showed that the environment in Yuxi area was more conducive to the increase of effective panicles, the environment of Kunming area and Yuxi area was more conducive to the increase of the number of filled grains, the environment of Baoshan area and Dali area was more conducive to the increase of 1000 grain weight, and the environment of Yuxi area and Lincang area was more conducive to the increase of yield; The number of grains was mainly controlled by heredity, secondly by the interaction between gene and environment, and the influence of environment was the least. The effective panicle, 1000 grain weight, yield and other yield traits were greatly affected by environment, and the interaction variation between gene and environment was greater than that of heredity. [Conclusion] The yield traits were affected by heredity, so it was still the key to improve the yield of feed barley. According to the yield performance of different varieties in different regions, the key to improve feed barley yield in different ecological regions was to select high-yield forage barley varieties for promotion. [ABSTRACT FROM AUTHOR]