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[Objective] The present paper aimed to study the impact of organic and inorganic combined application on the organic carbon composition, carbon bank management index and organic carbon functional group of tobacco planting soil in Wuxi county, so as to provide reference for improving the quality of tobacco planting soil and reasonable fertilization. [Method] Four treatments were set up under the condition of constant nitrogen input in field experiment: chemical fertilizer（CF）, chemical fertilizer with mushroom slag organic fertilizer（CMO）, chemical fertilizer with high carbon-based fertilizer（CHB）, and chemical fertilizer with biological organic fertilizer（CBO）. The effect of organic and inorganic application on soil carbon reservoir was studied by measuring total organic carbon and active carbon functional group structure using Fourier infrared spectrometry（FTIR）. [Result] （i） Compared with single organic fertilizer, the soil supplemented with organic fertilizer was slightly increased, and there was no significant difference between the treatments, significantly increased the output value on flue-cured tobacco leaf;（ii） The DOC, MBC content of the soil active carbon bank treated with organic fertilizer was significantly higher than that of single chemical fertilizer treatment. Among them, CMO treatment showed the largest increase in soil DOC content, thus being 67. 13%, 12. 84%, and 28. 54% higher than CF, CHB, CBO, respectively. CBO treatment showed the most increase in soil MBC content, with soil MBC content increasing 42. 32% compared to CF and CHB treatment showing 11. 43% higher soil EOC compared to CF;（iii）Among the three organic fertilizers, the DOC distribution of DOC, CMO, CBO, EOC, and the carbon bank management index of CHB treatment was significantly greater than CF and CMO;（iv） The structure difference of each treated organic carbon functional group was small, which mainly included phenols, aromatic hydrocarbons, olefins and mesamides. The relative peak area at 1634 cm-1 was associated significantly positively with soil MBC content, and that at 468 cm-1 was associated significantly negatively with soil TOC content. [Conclusion] Compared with single organic fertilizer, soil organic residue organic fertilizer significantly improved soil DOC and MBC content and high carbon-based fertilizer significantly improved soil EOC content and carbon bank management index. Short-term organic and inorganic distribution had little impact on organic carbon functional groups. However, the peaks at 1634 and 468 cm-1 were significantly associated with the soil TOC and MBC content. At the same time, organic-inorganic fertilizer significantly increased the yield and output value of flue-cured tobacco. [ABSTRACT FROM AUTHOR]