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The objectives of this study were to investigate the seed to seedling enhancement of Rhizo-product under laboratory conditions and to explore the efficacy of the product for partial fertilizer substitution in rice growth and yield production under submerged soil. Results showed that rice seedlings treated with Rhizo-product was significantly promoted compared to the non-bacterized control. Greenhouse experiments revealed that the growth parameters of all rice cultivars treated at 50% recommended fertilizer rate (RFR) and supplemented with Rhizo-product were better than, or equal to, the growth parameters of rice plants treated with full fertilizer rate (FFR) alone. Additionally, yield production of all rice cultivars receiving 50% RFR supplemented with the product was similar to rice treated with FFR alone. In conclusion, Rhizo-product stimulated early rice seed to seedling growth and exhibited growth and yield production at 50% RFR that was comparable to growth and yield production at FFR.