1. Functionalization of carbon nanoparticles modulates inflammatory cell recruitment and NLRP3 inflammasome activation.
- Author
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Yang M, Flavin K, Kopf I, Radics G, Hearnden CH, McManus GJ, Moran B, Villalta-Cerdas A, Echegoyen LA, Giordani S, and Lavelle EC
- Subjects
- Animals, Antigen-Presenting Cells, Caspase 1 metabolism, Dendritic Cells drug effects, Dose-Response Relationship, Drug, Female, Ligands, Macrophages drug effects, Mice, Mice, Inbred C57BL, Monocytes cytology, NLR Family, Pyrin Domain-Containing 3 Protein, Nanoparticles chemistry, Nanotechnology, Oxygen chemistry, Surface Properties, Carrier Proteins chemistry, Inflammation pathology, Nanotubes, Carbon chemistry
- Abstract
The inflammatory effects of carbon nanoparticles (NPs) are highly disputed. Here it is demonstrated that endotoxin-free preparations of raw carbon nanotubes (CNTs) are very limited in their capacity to promote inflammatory responses in vitro, as well as in vivo. Upon purification and selective oxidation of raw CNTs, a higher dispersibility is achieved in physiological solutions, but this process also enhances their inflammatory activity. In synergy with toll-like receptor (TLR) ligands, CNTs promote NLRP3 inflammasome activation and it is shown for the first time that this property extends to spherical carbon nano-onions (CNOs) of 6 nm in size. In contrast, the benzoic acid functionalization of purified CNTs and CNOs leads to significantly attenuated inflammatory properties. This is evidenced by a reduced secretion of the inflammatory cytokine IL-1β, and a pronounced decrease in the recruitment of neutrophils and monocytes following injection into mice. Collectively, these results reveal that the inflammatory properties of carbon NPs are highly dependent on their physicochemical characteristics and crucially, that chemical surface functionalization allows significant moderation of these properties., (Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Published
- 2013
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