1. Indirect, Non-Thermal Atmospheric Plasma Promotes Bacterial Killing in vitro and Wound Disinfection in vivo Using Monogenic and Polygenic Models of Type 2 Diabetes (Without Adverse Metabolic Complications).
- Author
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Cooley CR, McLain JM, Dupuy SD, Eder AE, Wintenberg M, Kelly-Wintenberg K, Wintenberg A, Collier JJ, Burke SJ, and Karlstad MD
- Subjects
- Animals, Diabetes Mellitus, Experimental genetics, Diabetes Mellitus, Experimental microbiology, Diabetes Mellitus, Experimental pathology, Diabetes Mellitus, Type 2 genetics, Diabetes Mellitus, Type 2 microbiology, Diabetes Mellitus, Type 2 pathology, Mice, Mice, Inbred BALB C, Mice, Obese, Pseudomonas Infections metabolism, Pseudomonas Infections pathology, Wound Infection microbiology, Wound Infection pathology, Wounds and Injuries microbiology, Wounds and Injuries pathology, Diabetes Mellitus, Experimental drug therapy, Diabetes Mellitus, Type 2 drug therapy, Disinfection, Plasma Gases pharmacology, Pseudomonas Infections drug therapy, Pseudomonas aeruginosa growth & development, Wound Infection drug therapy, Wounds and Injuries drug therapy
- Abstract
A novel atmospheric plasma device that uses indirect, non-thermal plasma generated from room air is being studied for its effects on wound disinfection in animal wounds of monogenic and polygenic murine models of type 2 diabetes. As a proof-of-concept report, the goal of this study was to demonstrate the efficacy and safety of the indirect non-thermal plasma (INTP) device in disinfecting polycarbonate filters established with Pseudomonas aeruginosa (PAO1) biofilms as well as wound disinfection in diabetic murine wounds. Dorsal excisional wounds in BALB/c, polygenic TALLYHO, and monogenic db/db mice established with PAO1 infection all demonstrated a 3-log colony-forming unit (CFU) reduction when subjected to a course of 20-min INTP treatments. Importantly, blood glucose and body weights in these animals were not significantly impacted by plasma treatment over the study period. Plasma safety was also analyzed via complete blood count and comprehensive metabolic panels, showing no deleterious systemic effects after 3 consecutive days of 20-min plasma applications. Therefore, the results obtained demonstrated the Pseudomonas aeruginosa isolates were highly sensitive to INTP in vitro, CFU reduction of infectious Pseudomonas in wounds of diabetic mice after INTP treatment is far superior to that of non-treated infected wounds, and the application of INTP shows no indication of toxic effects. Our results are consistent with indirect non-thermal atmospheric plasma as a promising adjunct to disinfecting wounds.
- Published
- 2020
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