1. [Research on the extracorporeal cytocompatibility of a composite of HA, carbon fiber and polyetheretherket-one]
- Author
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Xueyong, Liu, Chunbo, Deng, Jiquan, Liu, Jianjun, Li, and Guoxin, Sui
- Subjects
Osteoblasts ,Polymers ,Biocompatible Materials ,Prostheses and Implants ,Ketones ,Alkaline Phosphatase ,Carbon ,Polyethylene Glycols ,Rats ,Benzophenones ,Durapatite ,Orthopedics ,Carbon Fiber ,Animals ,Cells, Cultured ,Cell Proliferation - Abstract
The present research was to study the biocompatibility of a composite of hydroxyapatite (HA), carbon fiber (CF) and polyetheretherket-one (PEEK) by co-culturing with the osteoblasts in vitro. Cell relative growth (RGR) was used as a quantitative assessment for cytotoxicity of the biomaterials by CCK-8. The proliferation index of the co-cultured cells and ALP activity was measured to study the effect of PEEK-HA-CF composites. Morphological properties of the osteoblast cells in vitro were observed by scanning electro-microscopy (SEM). The PEEK-HA-CF materials have no cytotoxicity to osteoblasts. The proliferation index of PEEK-HA-CF was higher than that of Ti alloy group, but these was no significant difference compared to that of control group. The ALP activity was the highest on PEEK-HA-CF composites surface after 7 days. The osteoblast cells co-cultured with the PEEK-HA-CF composite were adhered well to the biomaterial as observed under the SEM. The results suggested that the PEEK-HA-CF composites had good biocompatibility in vitro and might be a novel orthopedic implanted material.
- Published
- 2012