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Objective To investigate the changes in serum 1，25-dihydroxyvitamin D3［1，25-(OH)2 D3］，gamma interferon（ INF- γ）and CD11b levels in children with rotavirus enteritis（RE）and their clinical significance. Methods Totally 108 children with RE were selected（RE group）and were divided into the mild（n=34），moderate（n=50）and severe（ n=24）groups according to the severity of dehydration，while 53 healthy children with physical examination were selected as the control group during the same period. Fasting venous blood was collected from the subjects in the early morning and 1，25-(OH)2 D3 and INF-γ were measured by enzyme-linked immunosorbent assay. Fully automated specific protein analyzer was used to detect the immunoglobulin A（IgA），IgG，IgM，flow cytometry was used to detect CD11b and CD3+T cells，CD4+T cells，and CD8+T cell percentages，and we calculated CD4+/CD8+. Pearson correlation was used to analyze the correlation between serum 1，25-(OH)2 D3，INF-γ and CD11b levels and humoral and cellular immune indicators in children with RE，and ROC curves were used to analyze the diagnostic value of each indicator for pediatric RE. Re⁃ sults Serum 1，25-(OH)2 D3 levels were lower and INF-γ and CD11b levels were higher in the RE group than in the control group（all P<0. 05）. Serum 1，25-(OH)2 D3 levels gradually decreased and INF- γ and CD11b levels gradually in‑ creased in the mild，moderate and severe groups（all P<0. 05）. IgA，IgG，IgM，CD3+T cells，CD4+T cells and CD4+/ CD8+ were reduced in the RE group as compared with those in the control group（all P<0. 05）. Serum 1，25-(OH)2 D3 levels in children with RE were positively correlated with IgA，IgG，IgM，CD3+T cells，CD4+T cells and CD4+/CD8+，while INF-γ and CD11b levels were negatively correlated with IgA，IgG，IgM，CD3+T cells，CD4+T cells and CD4+/CD8+（all P< 0. 05）. The best cut-off value for 1，25-(OH)2 D3 for pediatric RE was 25. 85 pg/mL，with an area under curve of 0. 806 （95%CI：0. 736-0. 864），and the sensitivity，specificity and accuracy were 85. 19%，64. 15% and 78. 26%，respectively； the best cut-off value for INF-γ for pediatric RE was 1. 67 pg/mL，with an area under curve of 0. 803（95%CI：0. 733 to 0. 862），the sensitivity of 74. 07%，specificity of 81. 13% and accuracy of 76. 39%，respectively；the best cut-off value for CD11b for pediatric RE was 75. 41%，with an area under curve of 0. 799（95%CI：0. 729 to 0. 858），and the sensitivity， specificity and accuracy were 68. 52%，81. 13%，and 72. 67%，respectively. Conclusion Serum 1，25-(OH)2 D3 levels are low and INF- γ and CD11b levels are high in children with RE；these three indicators can reflect the immune function and severity of dehydration in children，and have some diagnostic value for RE. [ABSTRACT FROM AUTHOR]

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