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Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To explore the effects of sleep-wake rhythm intervention on blood cells in mice with aplastic anemia and its possible mechanisms of action by observing the changes in peripheral blood cell count and plasma circadian rhythm regulatory factors (MT, NE) in mice with aplastic anemia whose sleep-wake rhythm has been intervened. Methods Male C57BL/6 mice were randomly divided into the experimental groups ［sleep-wake rhythm intervention groups (sleep intervention group, wakefulness intervention group, sleep-wake intervention group) and sleep-wake non-intervention group］ and normal control group. In the experimental groups, we established the models of aplastic anemia in mice, while the mice in the normal control group did not undergo the model induction. The sleep-wake rhythm was maintained normally in the sleep-wake rhythm non-intervention group and the normal control group. The mice in the wakefulness intervention group and sleep intervention group received 24-hour light exposure and 24-hour sleep intervention, respectively. In the sleep-wake intervention group, sleep and wakefulness patterns were reversed for the first 3 days and the opposite in the following 3 days. Each group underwent a 9-day intervention period. Peripheral blood cell counts were measured in all groups on the day before intervention and on the 6th day after intervention. ELISA was used to measure the levels of MT, NE, OX, and PGE2 in the plasma and bone marrow of mice in the experimental and control groups before intervention. Plasma MT and NE levels were measured in each group on the day before intervention and on the 3rd, 6th, and 9th days after intervention. Results ① Comparison of blood cell counts before and after intervention in each group： Prior to intervention, compared with the normal control group, the experimental group had lower WBC, RBC, PLT counts, and HGB levels （all P<0. 01). On the 6th day of sleep-wake rhythm intervention, compared with the normal control group, the sleep-wake rhythm non-intervention group and sleep intervention group had lower WBC, RBC, and HGB levels （all P< 0. 01). The wakefulness intervention group and sleep-wake intervention group had lower WBC and HGB levels, but higher PLT levels in comparison with those of the sleep-wake rhythm non-intervention group （all P<0. 01). Compared with the sleep-wake rhythm non-intervention group, the wakefulness intervention group and sleep-wake intervention group had higher RBC and HGB levels （all P<0. 01). Compared with the sleep intervention group, the wakefulness intervention group and sleep-wake intervention group had higher PLT levels （all P<0. 05). ② Comparison of plasma MT and NE levels before and after intervention in each group： On the 3rd day of intervention, compared with the normal control group, the sleepwake rhythm non-intervention group and wakefulness intervention group had higher plasma MT levels （all P<0. 01). Com‐ pared with the sleep-wake rhythm non-intervention group, the wakefulness intervention group had higher plasma MT level （P<0. 01), while the sleep-wake intervention group had lower plasma MT level （P<0. 05). Compared with the sleep intervention group, both the wakefulness intervention group and sleep-wake intervention group had higher plasma MT levels （both P<0. 05). On the 6th day of intervention, there were no statistically significant differences in plasma MT levels among the groups, but a pattern emerged with the sleep-wake intervention group > sleep-wake rhythm non-intervention group > normal control group. On the 3rd day of intervention, compared with the normal control group, the sleep-wake intervention group had higher plasma NE level （P<0. 05). Compared with the sleep-wake rhythm non-intervention group, sleep intervention group, and wakefulness intervention group, the sleep-wake intervention group had higher plasma NE levels （all P<0. 05). On the 6th day of intervention, compared with the normal control group, the wakefulness intervention group had higher plasma NE level （P<0. 05), while the sleep-wake intervention group had lower plasma NE level （P< 0. 05). On the 9th day of intervention, compared with the normal control group and sleep intervention group, the sleepwake intervention group had higher NE level （both P<0. 05). Conclusions In mice with aplastic anemia whose sleepwake rhythm is intervened, there is an increase in peripheral blood cell count, as well as elevated levels of plasma MT and NE, indicating intervention in both sleep and wakefulness rhythms. Disrupted sleep-wake rhythm intervention may contribute to the elevation of blood cell count in mice with aplastic anemia by regulating MT and NE levels. [ABSTRACT FROM AUTHOR]

Objective To investigate the predictive value of serum intestinal fatty-acid binding protein (I-FABP) and smooth muscle 22 (SM22) levels on intestinal necrosis in patients with acute mesenteric ischemia (AMI). Methods Totally 167 patients with AMI were divided into the intestinal necrosis group（58 cases) and non-intestinal necrosis group (109 cases). The clinical data of AMI patients were collected, and the serum I-FABP and SM22 were detected by ELISA. Multivariate Logistic regression was used to analyze the influencing factors for intestinal necrosis in AMI, and receiver operating characteristic (ROC) curve was used to analyze the predictive value of serum I-FABP and SM22 levels on intestinal necrosis in AMI. Results Multivariate Logistic regression analysis showed that lactic acid, leukocyte count, I-FABP and SM22 were the influencing factors for intestinal necrosis in AMI (all P<0. 05). The area under ROC curve of I-FABP + SM22 in predicting intestinal necrosis of AMI patients was greater than that of I-FABP and SM22 alone (both P<0. 05). Conclusion AMI patients with elevated serum levels of I-FABP and SM22 are prone to intestinal necrosis, and serum levels of I-FABP and SM22 can be used as predictors of intestinal necrosis in AMI. [ABSTRACT FROM AUTHOR]

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

目的 观察中药清胰利胆合剂灌肠对内镜下逆行胰胆管造影术(ERCP)术后胰腺炎(PEP)和高淀粉酶血症(PEH)的预防作用及对炎症因子IL-6、IL-8、IL-10的影响.方法 选取79例预行ERCP术患者,采用随机数字法分成中药组(40例)和西药组(39例).两组术前均常规治疗,中药组术前2 h给予清胰利胆合剂100 mL保留灌肠,西药组术前2 h给予吲哚美辛栓0.1 g塞肛.记录PEP和PEH发生例数,观察两组术前及术后4、24 h的血清淀粉酶、C反应蛋白(CRP)、降钙素原(PCT)、IL-6、IL-8、IL-10变化及患者术后腹痛、排气情况.结果 中药组与西药组PEP和PEH发生率比较差异无统计学意义(P均>0.05),手术前后腹痛评分比较差异无统计学意义(P均>0.05),血清CRP、PCT、IL-6、IL-8、IL-10水平比较差异无统计学意义(P均>0.05);中药组术后24 h血淀粉酶降低水平优于西药组(P<0.05);中药组术后排气时间早于西药组(P<0.05).结论 中药清胰利胆合剂灌肠预防PEP及PEH与西药疗效相当,能降低PEP及PEH的发生率,促使患者术后更快的排气,调节免疫功能,加强对胰腺的保护作用,在一定时间范围内降低血淀粉酶水平的效果优于西药;对炎症因子的影响不明显. [ABSTRACT FROM AUTHOR]

Objective To investigate the effects of osteoclast factors on pain-related protein expression in fetal dorsal root ganglion (DRG) cultured in vitro. Methods We raised 20 embryos to the 15tli day of pregnancy to perform embryo retrieval, and we extracted fetal rat DRG for in vitro culture for 48 hours and then they were randomly divided into 6 groups; the control group (containing 0.1% PBS), transforming growth factor (2, (TGF-β1 treatment group (containing 10 ng/mL TGF-β1 ) , insulin-like growth factor II (IGF-II ) treatment group (containing 1 μ/mL IGF-II ) , basic fibroblast growth factor (bFGF) treatment group (containing 10 ng/mL bFGF), platelet-derived growth factor (PDGF) AA treatment group ( containing 50 ng/mL PDGF-AA), and bone morphogenic protein 5 (BMP-5) treatment group (containing 10 μ/mL BMP-5). Western blotting and real-time fluorescence quantitative PGR were applied to analyze the protein and mRNA expression of galanin (Gal) , Gal receptor 1 ( GalRl ) , Gal receptor 2 ( GalR2 ), substance P (SP) , calcitonin gene-related peptide (CGRP). Results Compared with the control group, the protein expression of CGRP, Gal, and SP in the other groups was up-regulated (all P<0.05) , the GalRl protein expression in TGF-β1, group and PDGF-AA group was down-regulated (P<0.05), and the GalR2 protein expression in TGF-β1, group, bFGF group, and PDGF- A group was up-regulated (P<0.05). Compared with the control group, the relative mRNA expression levels of CGRP and Gal in the other treatment groups increased (all P<0.05) , the mRNA expression level of GalRl in the TGF-β1, group and PDGF-AA group decreased (P<0.05), the mRNA expression level of GalR2 in TGF-β1, group, bFGF group, and PDGF-AA group increased (P<0.05), and the mRNA expression level of SP in TGF-β1, group, IGF-II group, bFGF group, and PDGF-AA group increased (P<0.05). Conclusion Osteoclasts factors TGF-β1, , IGF-II , bFGF, PDGF-AA, and BMP-5 have an effect on the expression of pain-related proteins Gal, GalRl , GalR2, SP, and CGRP in DRG in vitro. [ABSTRACT FROM AUTHOR]

Objective To observe the expression of insulin-like growth factor 1(IGF-1) and insulin-like growth factor binding protein 3(IGFBP3) in the lung tissues of rats with chronic obstructive pulmonary disease(COPD), and to explore the mechanism of airway remodeling. Methods Twenty male Wistar rats were randomly divided into model group and control group, with 10 in each. We adopted the method of cigarette smoking to establish the COPD rat models. The rats were put into the smoking box with 10 cigarettes in, each time we lit them for 30 minutes, 3 times/d, for 90 days. The control group was fed normally. The rats were killed and lung tissues were taken, the histomorphological changes were observed by HE staining and the wall thickness of small airway was measured. The expression of IGF-1 and IGFBP3 protein in each group were detected by immunohistochemistry. The expression of IGF-1 and IGFBP3 mRNA was detected by real-time fluorescence quantitative PCR; Pearson method was used to analyze the correlation between IGF-1 and IGFBP3 protein expression and bronchiole wall thickness. Results Compared with the control group, the bronchiole wall thickness in the model group increased, and the protein and mRNA expression levels of IGF-1 and IGFBP3 in the lung tissues were enhanced(all P < 0.01). IGF-1 protein and IGFBP3 protein were both positively correlated with the thickness of bronchiole wall(r =0. 658 and 0. 654, respectively; all P < 0.01). IGF-1 protein was positively correlated with IGFBP3 protein(r = 0.810, P< 0.01). Conclusion The expression of IGF-1 and IGFBP3 in the lung tissues of COPD rats increases, which may be involved in the airway remodeling of COPD by promoting the proliferation of cells around the airway. [ABSTRACT FROM AUTHOR]

目的探讨磁共振成像小脑中脚宽度对多系统萎缩( MSA ) 症的诊断及与帕金森病( PD ) 鉴别诊断中的 效能。方法采用磁共振成像( MRI) 检查测量132 例MSA 患者（ 包括MSA-C 型及MSA-P 型） 和54 例PD 患者及 30 例正常对照者的小脑中脚宽度，并采用ROC 评估磁共振成像小脑中脚宽度对MSA 的诊断及与PD 的鉴别诊断 效能。结果MSA 患者双侧小脑中脚宽度[ ( 6. 73 土1 . 80 ) mm ] 小于正常对照者[ ( 10. 37 口17 ) mm) 及PD 患者 [ ( 9. 95 土1 . 88 ) mm] ( P 均< 0. 05 ) 。MSA-P 型患者小脑中脚宽度[ ( 7. 85 土1 . 86 ) mm ] 大于MSA-C 型[ ( 6 . 20 土 1 . 52 ) mm] ( P < 0. 05 ) 。磁共振成像小脑中脚宽度诊断MSA 的ROC 下AUC 为0. 945 ( P < 0. 05 ) ， 最住诊断界值为 8. 325 mm ， 敏感度94. 4% ， 特异度84. 8% 。磁共振成像小脑中脚宽度诊断MSA-C 型的ROC 下AUC 为0. 784 ， 最住 诊断界值7. 325 mm ， 敏感度69. 0% ， 特异度80. 0% 。结论磁共振成像小脑中脚宽度对MSA 有较好的诊断效能， 且有助于MSA 的分型及与PD 的鉴别诊断。 [ABSTRACT FROM AUTHOR]

目的总结乳腺多形性小叶癌(BPLC)的临床病理资料,以探讨其诊断要点、有效治疗方法及预后。方法对16例BPLC患者的临床病理资料作回顾性分析。结果患者年龄47～82岁、中位年龄68. 5岁。16例患者中,无意中发现乳腺包块就诊4例、发现乳腺包块伴刺痛就诊2例、偶尔发现乳腺包块就诊5例、健康体检发现乳腺包块就诊5例。病理学表现:①大体病理:肿块直径在1. 1～8. 6 cm、平均4. 31 cm。肿块质地均柔软偏硬,切面灰白色,均无包膜。有2例(2/16)可见点状的粉刺样坏死组织。②HE染色镜下所见:多形性癌细胞异型性明显,核较经典型小叶癌细胞核大2～3倍;细胞之间有黏附性;核分裂象6～21个/10 HPF;伴有多形性小叶原位癌2例、经典型小叶原位癌14例、经典型浸润性小叶癌5例;多形性小叶原位癌中见粉刺样坏死2例、脉管内见癌栓5例、神经侵犯5例;间质中及其周围有显著淋巴细胞浸润6例;周围导管内见Paget样浸润3例;腋窝淋巴结转移13例;远处转移2例。③免疫表型:多形性成分ER 2例、PR3例,C-erbB-2阳性(3+) 8例、C-erbB-2不定性(2+) 6例、Cerb B-2阴性(0或1+) 2例; E-cadherin阴性9例、胞膜弱阳性4例、E-cadherin胞质阳性3例; p120胞质阳性16例;CK5/6灶区阳性7例、16例Ki-67阳性细胞表达率30%～60%。HER2基因扩增10例。3例接受内分泌治疗,9例有HER2扩增者接受赫赛汀治疗,接受化疗4例(具体方案不详)。截止到2018年6月30日,1例失访,8例死亡、其中有远处转移者2例。结论 BPLC多发生在老年女性人群中,临床多表现为乳腺包块;免疫荧光染色结果显示E-cadherin阴性,p120阳性是本病主要的诊断依据;手术是本病主要治疗方法,HER2基因阳性者可接受靶向治疗;本病易于发生淋巴结转移,预后较差。 [ABSTRACT FROM AUTHOR]

Objective To investigate the effects of polypeptide extract from scorpion venom(PESV) on the Hedgehog(Hh) pathway downstream activating factor Gli1 expression of CML K562 cells. Methods The cultured K562 cells were inoculated in 24-well plate,and then the cells were divided into the following five groups: PESV group 1,PESV group 2,PESV group 3,imatinib group and the blank control group. The cells in the PESV groups 1,2,and 3 were added with 10,20,and 40 μg/m L PESV 20 μL,respectively,cells in imatinib group were added with 2 mg/m L imatinib 20 μL,and cells in the blank control group were added with saline 20 μL. At 48 h,the expression level of Gli1 mRNA was determined by PT-PCR,and the expression of Gli1 protein was determined by Western blotting. Results The relative expression levels of Gli1 mRNA in the PESV group 1,PESV group 2,PESV group 3,imatinib group,and the blank control group were 1. 983± 0. 091,1. 355 ± 0. 093,1. 279 ± 0. 105,1. 302 ± 0. 074,and 2. 745 ± 0. 188,respectively; the relative expression levels of Gli1 protein in each group were 0. 902 ± 0. 057,0. 722 ± 0. 074,0. 693 ± 0. 097,0. 701 ± 0. 067,and 0. 981 ±0. 063,respectively. There was no significant difference in the expression of Gli1 mRNA and protein between the PESV group 1 and the blank control group(all P > 0. 05),the relative expression levels of Gli1 mRNA and protein in PESV group 2,PESV group 3 and imatinib group were significantly lower than those of the PESV group 1 and the blank control group(all P < 0. 05),but there was no significant difference among PESV group 2,PESV group 3,and imatinib group(all P > 0. 05). Conclusion PESV [(20-40) μg/m L]can obviously inhibit the expression of Gli1 gene in K562 cells,and Gli1 gene is a target for the treatment of CML by PESV. [ABSTRACT FROM AUTHOR]

目的 观察泛素样含PHD和环指域1 (UHRF1)在喉鳞状细胞癌（简称喉鳞癌）组织中的表达情况，并探 讨其意义。方法选取82例择期行手术治疗的喉鳞癌患者，术中均留取喉鳞癌组织和癌旁正常组织，利用实时荧 光定量PCR检测喉鳞癌和癌旁正常组织中UHRF mRNA表达。以喉鳞癌组织中UHRF mRNA相对表达量的P25 作为界值，将患者分为低表达组和高表达组。采用Kaplan-Meier法对喉鳞癌组织中UHRF mRNA相对表达量对生 存时间的影响进行分析，采用多因素Cox风险回归模型对影响喉鳞癌患者预后的相关因素进行分析。结果喉鳞 癌、癌旁正常组织中UHRF mRNA相对表达量分别为1. 85 ±0. 16、. 17 ±0.13,两者比较<0.01。喉鳞癌组织 中UHRF1 mRNA相对表达量与病理学分级、T分期、临床分期和淋巴结转移有关(P均<0. 05)。低表达组23例， 高表达组59例，两组患者中位生存时间分别为44、32个月，两组比较，P <0.05。Cox回归模型结果显示，UHRF1 mRNA表达水平是喉鳞癌患者预后的独立影响因素（狀=2. 035,95%C/: 1. 024 ~ 4. 473, P = 0. 035 )。结论 UHRF1 mRNA在喉鳞癌组织中高表达，且与肿瘤恶性行为及患者预后有关。 [ABSTRACT FROM AUTHOR]

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

目的：观察卵巢癌组织中微小RNA-204（miR-204）表达变化，以及miR-204对卵巢癌SKOV3细胞凋亡的影响。方法选择卵巢癌患者50例，分别取其卵巢癌及相应癌旁组织；体外培养卵巢癌SKOV3细胞，随机分为1、2、3组，分别加入转染试剂、阴性对照载体、化学合成的双链miRNA-204表达载体；采用实时荧光定量PCR（qRT-PCR）法检测组织及细胞中miRNA-204相对表达量；观察miRNA表达与卵巢癌患者临床病理特征的关系；采用流式细胞仪检测卵巢癌SKOV3细胞凋亡情况。结果卵巢癌组织中miR-204 mRNA相对表达量低于癌旁组织（P＜0．01）；miR-204 mRNA表达与FIGO分期、淋巴结转移、CA125水平相关（P均＜0．05）。1、2、3组miRNA-204 mRNA相对表达量分别为2．35±0．67、2．01±0．45、5．32±0．88，3组较1、2组增加（P均＜0．05）；细胞凋亡率分别为7．32％±1．40％、5．14％±1．90％、52．4％±9．10％，3组较1、2组增加（P均＜0．05）。结论卵巢癌组织中miR-NA-204表达下调，且与病情进展相关；miRNA-204促进卵巢癌细胞凋亡，这可能是其发挥抑癌作用的机制之一。 [ABSTRACT FROM AUTHOR]