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Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To explore the effects of long non-coding RNA(lncRNA) small nucleolar RNA host gene 16(SNHG16) knockdown on the apoptosis of gastric cancer AGS cells and its possible mechanism. Methods Well-cultured gastric cancer AGS cells in the third generation was randomly divided into three groups: the blank control group, negative control group, and si-SNHG16 group. Blank medium, medium with negative control si-NC and medium with interference si-SNHG16 were added into the blank control group, negative control group, and si-SNHG16 group, respectively. After transfection with si-SNHG16 for 48 h in the gastric cancer AGS cells, the interference efficiency of SNHG16 and the expression of p53 gene after SNHG16 knockdown were detected by qRT-PCR assay. The apoptosis was determined by Annexin V-FITC/PI double staining flow cytometry and Hoechst 33342 staining. The expression of p53, Bcl-2, Bax and Cleaved Caspase-3 protein was evaluated by Western blotting. The activity of Caspase-3 enzyme was detected by spectrophotometry assay. Results At 48 h after transfection, compared with the blank control group and negative control group, the relative expression of lncRNA SNHG16 decreased, and the relative expression of p53 gene increased in the si-SNHG16 group(both P<0.05). Besides, the apoptosis rate and apoptotic cells of the si-SNHG 16 group all increased(all P<0.05). The expression of p53 protein and apoptosis-related proteins(Bax and Cleaved caspase-3) were up-regulated significantly(all P<0.05), but the expression of Bcl-2 was down-regulated(P<0.05). Moreover, the activity of Caspase-3 enzyme was enhanced(P<0.05). No significant difference was found in the above parameters between the blank control group and negative control group(all P>0.05).Conclusion LncRNA SNHG16 knockdown could promote the apoptosis of gastric cancer AGS cells by activating the p53 signaling pathway downstream apoptosis-related protein expression and thus initiating the apoptosis. [ABSTRACT FROM AUTHOR]

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)