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目的 观察纤维支气管镜肺泡灌洗术辅助治疗≥6 岁小儿重度急性发作期支气管哮喘的临床疗效。 方法 将68例≥6岁重度急性发作期支气管哮喘患儿随机分为观察组和对照组各34例，对照组根据指南给予常规 治疗，观察组在对照组基础上行纤维支气管镜肺泡灌洗术。于治疗后 24 h评价两组临床疗效，于治疗前及治疗后 6、24 h检测两组血气指标［氧分压 (PaO2 )、二氧化碳分压 (PaCO2 )、血氧饱和度 (SaO2 )］及血清白细胞介素 8 (IL-8)、 肿瘤坏死因子 α (TNF-α)，于治疗前及治疗后 24 h检查两组肺功能［第 1秒肺呼气容积 (FEV1 )、第 1秒肺呼气容积/ 用力肺活量 (FEV1/FVC)、呼气峰流速 (PEF)］，记录两组哮鸣音消失时间、住院时间及插管并发症。结果 观察组 总有效率 94. 12%，高于对照组的 76. 47% (P<0. 05)。治疗前两组血气指标、肺功能指标及血清 IL-8、TNF-α水平比 较均无统计学差异 (P均>0. 05)，治疗后两组PaO2、SaO2及肺功能指标均升高而PaCO2及血清IL-8、TNF-α水平降低， 且治疗后观察组各指标变化更明显 (P均<0. 05)。观察组哮鸣音消失时间及住院时间均短于对照组 (P均<0. 05)。 观察组出现低氧血症4例，经上调氧流量后缓解，未出现严重插管并发症。结论 纤维支气管镜肺泡灌洗术辅助治 疗≥6岁小儿重度急性发作期支气管哮喘的临床疗效较好，有助于促进肺通气功能恢复、降低机体炎症反应，从而促 进患儿更早康复。 [ABSTRACT FROM AUTHOR]

目的 探讨穴位埋线联合椎间盘突出部位注射医用臭氧及甲强龙治疗根性坐骨神经痛的临床效果。方 法 选择 108 例椎间盘突出导致根性坐骨神经痛患者, 按照治疗方式不同分为观察组和对照组各 54 例。对照组选 取突出部位, 依次注射医用臭氧 5 mL (30 µg/mL) 、甲强龙20 mg, 每周 1 次, 持续 3 个月。观察组严格按照中医辨证 分型标准分成四种类型, 在以上药物注射治疗基础上均以大肠俞、环跳、委中、阳陵泉为主穴进行穴位埋线, 再根据 不同证型增加两个辅穴行穴位埋线 (寒湿证增加昆仑、秩边, 湿热证增加飞扬、丰隆, 肝肾不足证增加脾俞、肾俞, 气 滞血瘀证增加膈俞、次髎), 每周 1 次, 持续 3 个月。比较两组治疗前后疼痛分级指数 (PRI) 评分、视觉模拟评分法 (VAS) 评分、行为疼痛 6 点评分 (BRS-6) 、Oswestry 功能障碍指数 (ODI) 评分, 观察两组治疗效果及不良反应发生情 况。结果 治疗前两组各类观察项目评分比较差异均无统计学意义 (P均>0.05) 。治疗 3 个月, 观察组 PRI 感觉评 分、PRI情感评分、PRI 总分、VAS 评分、BRS-6 评分、ODI 评分均低于对照组, 观察组总有效率高于对照组, 差异具有 统计学意义 (P均<0.05) ；两组不良反应发生率比较差异无统计学意义 (P>0. 05)。结论 中医穴位埋线联合医用臭 氧及甲强龙注射治疗椎间盘突出致根性坐骨神经痛, 可以明显缓解患者肢体疼痛, 改善其肢体功能受限, 且无不良 反应发生率升高情况。 [ABSTRACT FROM AUTHOR]

Objective To observe the effects of adiponectin（APN）on angiotensin-Ⅱ（Ang Ⅱ）-induced cardiac fibroblasts（ CFs）proliferation and extracellular signal-regulated kinase 1/2（ERK1/2）activation. Methods Cardiac ventricles were separated from 1 to 3-day-old C57 mice. Pure primary mouse cardiac fibroblasts were obtained after cardiomyocytes were separated by trypsin-collagenase type I digestion and differential adhesion. Morphological and immunocytochemical technique was used to identify the CFs. CFs of two to four generations were randomly divided into five groups. CFs in the control group were cultured with serum-free DMEM medium. CFs in the Ang II group were cultured with DMEM medium containing 10-7 mol/L Ang II. CFs in the low-dose，medium-dose and high-dose APN groups were treated with 1，5 and 25 μg/mL APN for 1 h，respectively，and then were cultured with DMEM medium containing 10-7 mol/L Ang II. The cells were collected after 24 h culture. The proliferation ability（OD）of CFs was measured by MTT，the expression of ERK1/2 and phosphorylated extracellular signal-regulated kinase 1/2（p-ERK1/2）in CFs was detected by Western blotting，and the p-ERK/ERK ratio was calculated. Results CFs were successfully isolated from mouse heart， and the cell purity was close to 100% by immunofluorescence combined with morphological identification. Compared with the control group，the OD value of the Ang II group increased（P<0. 05）；compared with the Ang II group，OD values of the APN groups were lower（all P<0. 05）；there was no statistically significant difference in OD value among APN groups （P>0. 05）. There was no statistically significant difference in the relative expression of ERK1/2 protein among all groups （P>0. 05）. Compared with the control group，the relative expression of p-ERK protein and the ratio of p-ERK/ERK were higher in the Ang II group（all P<0. 05）. Compared with the Ang II group，p-ERK protein relative expression level and the ratio of p-ERK/ERK were lower in the high-dose APN group（all P<0. 05）. Conclusion Adiponectin inhibits the proliferation of CFs and the activation of ERK1/2 signal induced by Ang II. [ABSTRACT FROM AUTHOR]

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目的 比较脾切除门奇静脉断流术中鼻腔、静脉给予不同剂量布托啡诺复合丙泊酚的麻醉效果。方法150例择期行脾切除门奇静脉断流术患者随机分为5组各30例,A组直接以丙泊酚作为诱导剂,在手术过程中保持适当的麻醉深度,根据患者反应追加丙泊酚;B、C组在给予丙泊酚之前,鼻腔给予1、2 mg布托啡诺;D、E组在给予丙泊酚之前,静注1、2 mg布托啡诺。比较各组麻醉效果。结果与A组比较,B、C、D、E组麻醉效果好,患者舒适度、术者满意度高;与D组比较,B组麻醉效果好,患者舒适度、术者满意度高;与E组比较,D组麻醉效果差,患者舒适度、术者满意度低;P均0.05。与同组T0时点比较,各组T2~T5 时点MAP、HR降低;与同组T0、T1、T5 时点比较,各组T2~T4 时点SpO2 降低;与A组比较,C、E组T2~T4 时点SpO2 降低;与E组比较,C组T2~T4 时点SpO2 降低;P均0.05。与A组比较,B、C、D、E组丙泊酚用量少;与D组比较,B组丙泊酚用量少;与E组比较,C组丙泊酚用量少;P均0.05。结论布托啡诺复合丙泊酚用于脾切除门奇静脉断流术麻醉效果较好,相同剂量的布托啡诺经鼻腔给药效果优于静注给药,且高剂量效果优于低剂量。 [ABSTRACT FROM AUTHOR]

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

自噬是真核细胞内降解异常蛋白质以及受损细胞器的一种生理过程,受多条信号通路调控,并参与多种疾病的发生发展。近年研究发现,白藜芦醇的药理作用与调控细胞自噬有关。白藜芦醇通过影响Akt-mTORC1、AMPK-Sirt1、Sirt1/Sirt3-FoxO以及STAT3信号通路发挥对细胞自噬的调控作用,通过调节AMPK-Sirt1-自噬通路发挥防治心血管疾病和神经系统疾病的作用,通过调节Akt-mTORC1-自噬通路以及STAT3-自噬通路发挥抗肿瘤作用,此外,白藜芦醇调控的Sirt1/Sirt3-FoxO-自噬通路能调节细胞能量代谢、缓解氧化应激损伤。 [ABSTRACT FROM AUTHOR]

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)