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Abstract: In this study, we evaluated the effect of reactor type with increased surface area on the fractional precipitation process for the efficient purification of paclitaxel. The three types of reactors (spiral, round, and twisted types) all exhibited higher yields than in cases where the surface area had not been increased at the same precipitation time. It was possible to obtain a high paclitaxel yield of 70% or above after 4h of precipitation. In particular, in the case of the spiral-type reactor, high yields of 64% and 72%, respectively, were obtained 2h and 4h after precipitation, thus exhibiting the highest precipitation efficiency. In addition, it was also possible to dramatically reduce the time necessary for fractional precipitation. On the other hand, the reactor type hardly affected the purity. The size of the paclitaxel precipitate increased with precipitation time. With the spiral-, round-, and twisted-type reactors, it was possible to obtain paclitaxel precipitates with smaller sizes than cases where the surface area had not been increased. [Copyright &y& Elsevier]

Abstract: A fast and simple solvent system screening method was established based on average polarity, which was much easier to find a suitable solvent system for high-speed counter-current chromatography (HSCCC) separation. Six alkaloids were separated and purified simultaneously with n-hexane–ethyl acetate–methanol–water (2:5:2:5) on HSCCC in one step. As a result, 4.7mg palmatine, 7.1mg berberine, 0.8mg worenine, 1.5mg epiberberine, 1.9mg coptisine and 0.6mg jatrorrhizine from 20mg Rhizoma coptidis extract were obtained with the purities of 98.5%, 94.1%, 90.4%, 95.5%, 88.4% and 91.1% respectively as determined by high-performance liquid chromatography (HPLC), and they were characterized by MS and 1H NMR. [Copyright &y& Elsevier]