1. Non-Invasive Method to Detect Infection with Mycobacterium tuberculosis Complex in Wild Boar by Measurement of Volatile Organic Compounds Obtained from Feces with an Electronic Nose System.
- Author
-
de Jesús Beleño-Sáenz K, Cáceres-Tarazona JM, Nol P, Jaimes-Mogollón AL, Gualdrón-Guerrero OE, Durán-Acevedo CM, Barasona JA, Vicente J, Torres MJ, Welearegay TG, Österlund L, Rhyan J, and Ionescu R
- Subjects
- Animals, Animals, Wild, Cattle, Feces, Female, Gold, Humans, Male, Spain, Sus scrofa, Swine, Electronic Nose, Metal Nanoparticles, Mycobacterium tuberculosis, Tuberculosis diagnosis, Tuberculosis veterinary, Volatile Organic Compounds
- Abstract
More effective methods to detect bovine tuberculosis, caused by Mycobacterium bovis , in wildlife, is of paramount importance for preventing disease spread to other wild animals, livestock, and human beings. In this study, we analyzed the volatile organic compounds emitted by fecal samples collected from free-ranging wild boar captured in Doñana National Park, Spain, with an electronic nose system based on organically-functionalized gold nanoparticles. The animals were separated by the age group for performing the analysis. Adult (>24 months) and sub-adult (12-24 months) animals were anesthetized before sample collection, whereas the juvenile (<12 months) animals were manually restrained while collecting the sample. Good accuracy was obtained for the adult and sub-adult classification models: 100% during the training phase and 88.9% during the testing phase for the adult animals, and 100% during both the training and testing phase for the sub-adult animals, respectively. The results obtained could be important for the further development of a non-invasive and less expensive detection method of bovine tuberculosis in wildlife populations.
- Published
- 2021
- Full Text
- View/download PDF