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The reactive oxygen species (ROS) including hypochlorous acid (HOCl), on the one hand they can regulate physiological function in our living organisms, on the other hand abnormal concentration of ROS would induce a series of biomacromolecules damage and further endanger virtually all aspects of human health. Especially it can be directly related to cancer. Thus to elucidate and visualize their concentration is very imminent. Before many fluorescent probes were used to detect HOCl. In recent years, near-infrared or ratiometric fluorescent developed prosperously. The strategy of combining near-infrared and ratiometric fluorescent probe is overwhelming in the design of fluorescent probes. In this work, we successfully designed and obtained a near-infrared and ratiometric fluorescent probe with large stokes by reacting isophorone with coumarin, which can monitor HOCl high efficiently with quick response and low detection limit. In addition, cell imaging experiments show that the probe can identify endogenous and exogenous ClO− successfully, and nude mouse imaging experiments show that the probe can detect exogenous ClO−. It is possible that the probe can be applied in early clinical diagnosis.

Because of the high toxicity of hydrazine, the development of ultrasensitive fluorescent probes for hydrazine has been challenging. In this work, an ultrasensitive fluorescent probe based on o-phthalaldehyde has been exploited for hydrazine. The probe was designed based on creating an efficient fluorophore by incorporating hydrazine into o-phthalaldehyde framework via an intermolecular condensation-cyclization reaction. The probe could quickly detect hydrazine and displayed a high sensitivity for hydrazine with a LOD of 1.9 nM (0.06 ppb), which is far below the U.S. Environmental Protection Agency standard (10 ppb). Moreover, the probe was successfully applied to the detection of hydrazine in real water samples and hydrazine vapor.

Cysteine (Cys), Glutathione (GSH) can resist the reactive oxygen species due to adversity and aging in plants. Thus, the specific detection of individual thiol in plants is very important. However, just a few have been used to discrimination of Cys/Hcy and GSH in vivo. Not to mention the discrimination detection for them in plants. In this work, we synthesized a potential promoting tandem reaction type fluorescent probe based on benzene-conjugated benzopylium ion and NBD-Cl. The probe could detect Cys/Hcy and GSH with high selectivity and low detection limit. More interesting, firstly we employed the probe to successfully detect Cys/Hcy and GSH in arabidopsis thaliana. Our research is useful for studying the conversion and metabolic pathways of thiols in plants.