1. Direct laser scribing of AgNPs@RGO biochip as a reusable SERS sensor for DNA detection.
- Author
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Han, Bing, Zhang, Yong-Lai, Zhu, Lin, Chen, Xu-Hui, Ma, Zhuo-Chen, Zhang, Xu-Lin, Wang, Jian-Nan, Wang, Wei, Liu, Yu-Qing, Chen, Qi-Dai, and Sun, Hong-Bo
- Subjects
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SERS spectroscopy , *GRAPHENE oxide , *MICROFLUIDIC analytical techniques , *MOLECULAR recognition , *MICROFLUIDICS - Abstract
The combination of surface-enhanced Raman spectroscopy (SERS) technology with microfluidics makes it possible to diagnose genetic disease through label-free on-chip DNA detection. However, open problems including the integration of SERS substrate with microfluidic devices, controllable trapping and releasing of target molecules are still challenging. Here we demonstrate a facile laser scribing method to fabricate silver nanoparticles (AgNPs) and graphene oxide (GO) based biochips as a reusable SERS sensor for DNA detection. Programmable laser scribing of the AgNPs@GO composite film enables direct patterning of sensitive SERS channels that consist of graphene supported AgNPs by exfoliating the composites into hierarchical porous structures. Integrating the SERS-active patterns with a microfluidic chip forms a biochip for allowing SERS detection of DNA. The noncovalent interactions between DNA and graphene mediated controllable trapping and releasing of DNA sequences, enabling efficient on-chip SERS detection and the regeneration of the biochip. The simple, green and cost-effective fabrication of the SERS-active biochips reveals great potential for biomolecular sensing and genetic engineering applications. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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