1. Improve the spatial resolution of fiber photometry by μLED linear array for fluorescence detection.
- Author
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Li, Yamin, Zhan, Lijie, wang, Yang, Chen, Ruru, Yang, Xiaowei, Wu, Xiaoting, Wang, Yijun, Chen, Hongda, Xu, Chun, and Pei, Weihua
- Subjects
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SPATIAL resolution , *FLUORESCENCE , *GREEN fluorescent protein , *PHOTOMETRY , *LUMINOUS flux , *OPTICAL fibers - Abstract
[Display omitted] • A method to detect the Ca2+ fluorescence intensity by integrating μLED linear array and optical fiber with depth resolution is proposed. • The excitation range of each μLED in the μLED linear array can't overlap with each other by controlling the luminous intensity. • When the μLED linear array turns on alternately, the excitation light has depth resolution. • Fluorescence at different location can be collected by an optical fiber. • Processing the fluorescence signals by the time-division multiplexing method, the fluorescence signals at a different location can be obtained. Calcium (Ca2+) fluorescence is widely used to monitor the activity of neurons in vivo. We propose a method of detection the Ca2+ fluorescence intensity by integrating a μLED linear array and an optical fiber. Due to the different positions of each μLED, the range of excitation of each μLED cannot overlap that of another by controlling the luminous intensity. When each μLED in a linear array is switched on in turn, only one μLED is emitting at any given time. In this way, the fluorescence from the active neurons collected by a single fiber can correspond to the position of the μLED according to the time sequence of each μLED, thus resulting in the relative position of the recorded fluorescence being distinguished. When the scanning speed of a μLED is much higher than the fluorescence process associated with Ca2+ activity, the device can simultaneously obtain the Ca2+fluorescence activity signals from multiple sites, thus inferring the activity of neurons through the fluorescence intensity, so that the neuron activity with depth resolution can be obtained. In the present work, the prototype device and system are constructed, and the method has been verified by simulation in vitro and green fluorescent protein (GFP) in vivo. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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