1. A Fluorescent One-Dimensional Photonic Crystal for Label-Free Biosensing Based on Bloch Surface Waves
- Author
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Maria Alvaro, Lucia Napione, Norbert Danz, Peter Munzert, Francesco Michelotti, Emiliano Descrovi, Fabrizio Giorgis, Valeria Moi, Paola Rivolo, Serena Ricciardi, Francesca Frascella, and Federico Bussolino
- Subjects
surface electromagnetic waves ,label-free sensing ,fluorescence ,multilayers ,bragg mirrors ,Chemical technology ,TP1-1185 - Abstract
A one-dimensional photonic crystal (1DPC) based on a planar stack of dielectric layers is used as an optical transducer for biosensing, upon the coupling of TE-polarized Bloch Surface Waves (BSW). The structure is tailored with a polymeric layer providing a chemical functionality facilitating the covalent binding of orienting proteins needed for a subsequent grafting of antibodies in an immunoassay detection scheme. The polymeric layer is impregnated with Cy3 dye, in such a way that the photonic structure can exhibit an emissive behavior. The BSW-coupled fluorescence shift is used as a means for detecting refractive index variations occurring at the 1DPC surface, according to a label-free concept. The proposed working principle is successfully demonstrated in real-time tracking of protein G covalent binding on the 1DPC surface within a fluidic cell.
- Published
- 2013
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