1. The microRNA156 and microRNA172 gene regulation cascades at post-germinative stages in Arabidopsis.
- Author
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Martin, Ruth C., Asahina, Masashi, Po-Pu Liu, Kristof, Jessica R., Coppersmith, Jennifer L., Pluskota, Wioletta E., Bassel, George W., Goloviznina, Natalya A., Nguyen, Theresa T., Martínez-Andújar, Cristina, Kumar, M. B. Arun, Pupel, Piotr, and Nonogaki, Hiroyuki
- Subjects
ARABIDOPSIS ,CASCADES (Fluid dynamics) ,GERMINATION ,CARRIER proteins ,SEEDLINGS ,POLYMERASE chain reaction - Abstract
MicroRNAs (miRNAs) are involved in developmental programmes of plants, including seed germination and post-germination. Here, we provide evidence that two different miRNA pathways, miR156 and miR172, interact during the post-germination stages in Arabidopsis. Mutant seedlings expressing miR156-resistant SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE13 (mSPL13), which has silent mutations in the miR156 complementary sequence, over-accumulated SPL13 mRNA and exhibited a delay in seedling development. Microarray analysis indicated that SCHNARCHZAPFEN (SNZ), an AP2-like gene targeted by miR172, was downregulated in these mutants. Quantitative reverse transcription- polymerase chain reaction (RT-PCR) and miRNA gel blot analyses showed that the MIR172 genes were up-regulated in mSPL13 mutants. These results suggest that the miRNA regulation cascades (miR156 s SPL13 → miR172 ⊣ SNZ) play a critical role during the post-germination developmental stages in Arabidopsis. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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