1. Axial plane optical microscopy.
- Author
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Li, Tongcang, Ota, Sadao, Kim, Jeongmin, Wong, Zi Jing, Wang, Yuan, Yin, Xiaobo, and Zhang, Xiang
- Subjects
Imaging ,Three-Dimensional ,Microscopy ,Image Enhancement ,Sensitivity and Specificity ,Reproducibility of Results ,Equipment Design ,Equipment Failure Analysis ,Lenses ,Imaging ,Three-Dimensional - Abstract
We present axial plane optical microscopy (APOM) that can, in contrast to conventional microscopy, directly image a sample's cross-section parallel to the optical axis of an objective lens without scanning. APOM combined with conventional microscopy simultaneously provides two orthogonal images of a 3D sample. More importantly, APOM uses only a single lens near the sample to achieve selective-plane illumination microscopy, as we demonstrated by three-dimensional (3D) imaging of fluorescent pollens and brain slices. This technique allows fast, high-contrast, and convenient 3D imaging of structures that are hundreds of microns beneath the surfaces of large biological tissues.
- Published
- 2014