169 results on '"Xin, Yi"'
Search Results
2. Correlation of procalcitonin and c-reactive protein levels with pathogen distribution and infection localization in urinary tract infections
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Jing Shi, Zhi-Song Zhan, Zu-Shun Zheng, Xue-Xia Zhu, Xin-Yi Zhou, and Shi-Yan Zhang
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Medicine ,Science - Abstract
Abstract Aimed to explore the relationships between infection localization, bacterial species, and procalcitonin (PCT) and C-reactive protein (CRP) levels in urinary tract infections (UTIs). A retrospective study included 314 UTI hospitalized patients divided into two groups (268 with lower UTI, 46 with upper UTI) in a tertiary care hospital. PCT and CRP were performed. Bacterial isolates were identified using standard microbiological techniques, and statistical analyses were performed to assess associations between infection localization, bacterial species, PCT, and CRP levels. Age and gender showed no significant differences between the lower and upper UTIs. Escherichia coli dominated as the leading UTI pathogen. A positive correlation (r = 0.646, P
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- 2023
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3. Correlation of procalcitonin and c-reactive protein levels with pathogen distribution and infection localization in urinary tract infections
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Shi, Jing, Zhan, Zhi-Song, Zheng, Zu-Shun, Zhu, Xue-Xia, Zhou, Xin-Yi, and Zhang, Shi-Yan
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- 2023
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4. Transcriptomics-based investigation of manganese dioxide nanoparticle toxicity in rats’ choroid plexus
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Meng, Chun-Yan, Ma, Xin-Yi, Xu, Ming-Yan, Pei, Sheng-Fei, Liu, Yang, Hao, Zhuo-Lu, Li, Qing-Zhao, and Feng, Fu-Min
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- 2023
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5. Pre-transplant infusion of donor leukocytes treated with extracorporeal photochemotherapy induces immune hypo-responsiveness and long-term allograft survival in murine models
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Schneiderman, Jennifer, Qiu, Longhui, Yeap, Xin Yi, Kang, Xin, Zheng, Feibo, Ye, Junsheng, Xie, Yan, Wang, Jiao-Jing, Sambandam, Yuvaraj, Mathew, James, Li, Lin, Leventhal, Joseph, Edelson, Richard L, and Zhang, Zheng Jenny
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Biomedical and Clinical Sciences ,Clinical Sciences ,Immunology ,Organ Transplantation ,Kidney Disease ,Transplantation ,6.1 Pharmaceuticals ,Development of treatments and therapeutic interventions ,Evaluation of treatments and therapeutic interventions ,5.2 Cellular and gene therapies ,Allografts ,Animals ,Disease Models ,Animal ,Graft Rejection ,Graft Survival ,Humans ,Mice ,Photopheresis ,T-Lymphocytes ,Regulatory ,Transplantation ,Homologous - Abstract
Recipients of solid organ transplantation (SOT) rely on life-long immunosuppression (IS), which is associated with significant side effects. Extracorporeal photochemotherapy (ECP) is a safe, existing cellular therapy used to treat transplant rejection by modulating the recipient's own blood cells. We sought to induce donor-specific hypo-responsiveness of SOT recipients by infusing ECP-treated donor leukocytes prior to transplant. To this end, we utilized major histocompatibility complex mismatched rodent models of allogeneic cardiac, liver, and kidney transplantation to test this novel strategy. Leukocytes isolated from donor-matched spleens for ECP treatment (ECP-DL) were infused into transplant recipients seven days prior to SOT. Pre-transplant infusion of ECP-DL without additional IS was associated with prolonged graft survival in all models. This innovative approach promoted the production of tolerogenic dendritic cells and regulatory T-cells with subsequent inhibition of T-cell priming and differentiation, along with a significant reduction of donor-specific T-cells in the spleen and grafts of treated animals. This new application of donor-type ECP-treated leukocytes provides insight into the mechanisms behind ECP-induced immunoregulation and holds significant promise in the prevention of graft rejection and reduction in need of global immune suppressive therapy in patients following SOT.
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- 2022
6. Author Correction: Transcriptomics-based investigation of manganese dioxide nanoparticle toxicity in rats’ choroid plexus
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Chun-Yan Meng, Xin-Yi Ma, Ming-Yan Xu, Sheng-Fei Pei, Yang Liu, Zhuo-Lu Hao, Qing-Zhao Li, and Fu-Min Feng
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Medicine ,Science - Published
- 2024
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7. Transcriptomics-based investigation of manganese dioxide nanoparticle toxicity in rats’ choroid plexus
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Chun-Yan Meng, Xin-Yi Ma, Ming-Yan Xu, Sheng-Fei Pei, Yang Liu, Zhuo-Lu Hao, Qing-Zhao Li, and Fu-Min Feng
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Medicine ,Science - Abstract
Abstract Manganese dioxide nanoparticles (MnO2-NPs) have a wide range of applications in biomedicine. Given this widespread usage, it is worth noting that MnO2-NPs are definitely toxic, especially to the brain. However, the damage caused by MnO2-NPs to the choroid plexus (CP) and to the brain after crossing CP epithelial cells has not been elucidated. Therefore, this study aims to investigate these effects and elucidate potential underlying mechanisms through transcriptomics analysis. To achieve this objective, eighteen SD rats were randomly divided into three groups: the control group (control), low-dose exposure group (low-dose) and high-dose exposure group (high-dose). Animals in the two treated groups were administered with two concentrations of MnO2-NPs (200 mg kg−1 BW and 400 mg kg−1 BW) using a noninvasive intratracheal injection method once a week for three months. Finally, the neural behavior of all the animals was tested using a hot plate tester, open-field test and Y-type electric maze. The morphological characteristics of the CP and hippocampus were observed by H&E stain, and the transcriptome of CP tissues was analysed by transcriptome sequencing. The representative differentially expressed genes were quantified by qRT-PCR. We found that treatment with MnO2-NPs could induce learning capacity and memory faculty decline and destroy the structure of hippocampal and CP cells in rats. High doses of MnO2-NPs had a more obvious destructive capacity. For transcriptomic analysis, we found that there were significant differences in the numbers and types of differential genes in CP between the low- and high-dose groups compared to the control. Through GO terms and KEGG analysis, high-dose MnO2-NPs significantly affected the expression of transporters, ion channel proteins, and ribosomal proteins. There were 17 common differentially expressed genes. Most of them were transporter and binding genes on the cell membrane, and some of them had kinase activity. Three genes, Brinp, Synpr and Crmp1, were selected for qRT-PCR to confirm their expression differences among the three groups. In conclusion, high-dose MnO2-NPs exposure induced abnormal neurobehaviour, impaired memory function, destroyed the structure of the CP and changed its transcriptome in rats. The most significant DEGs in the CP were within the transport system.
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- 2023
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8. The association between dose of hemodialysis and patients mortality in a prospective cohort study
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Shu-Xin Liu, Zhi-Hong Wang, Shuang Zhang, Jia Xiao, Lian-Lian You, Yu Zhang, Cui Dong, Xue-Na Wang, Zhen-Zhen Wang, Sheng-Nan Wang, Jia-Ni Song, Xiu-Nan Zhao, Xin-Yi Yan, Shu-Fan Yu, and Yi-Nan Zhang
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Medicine ,Science - Abstract
Abstract Dialysis adequacy is a known risk factor for mortality in maintenance hemodialysis (MHD) patients. However, the optimal dialysis dose remains controversial. Therefore, we aimed to explore the relationship between dialysis dose and all-cause and cardiovascular disease (CVD) mortality among MHD. We examined the associations of dialysis dose with mortality in a cohort (n = 558) of MHD patients from 31 December 2015 to 31 December 2020. Dialysis adequacy was assessed using baseline Single-pool Kt/Vurea (spKt/V), which was categorized into three groups, and the lowest dose group was used as the reference category. Hazard ratios (HRs) and 95% confidence intervals (CIs) were calculated using Cox proportional hazards regression models. A total of 214 patients died (64.5% for CVD). Compared with the low-dose group, high-dose group could reduce the risk of all-cause mortality by 33% (HR = 0.67, 95% CI: 0.47–0.98). Of note, when stratification by age, high-dose group was associated with both lower all-cause (HR = 0.46, 95% CI: 0.26–0.81) and CVD mortality (HR = 0.42, 95% CI: 0.20–0.88) among patients with age below 65 years. When stratification by dialysis age, high-dose group was associated with decreased risk of CVD mortality (HR = 0.43, 95% CI: 0.20–0.91) among patients with dialysis age over 60 months. spKt/V is a simple index of hemodialysis dose used in clinical practice and a useful modifiable factor in predicting the risk of death, especially in MHD patients under 65 years old or dialysis age more than 60 months.
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- 2022
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9. Pre-transplant infusion of donor leukocytes treated with extracorporeal photochemotherapy induces immune hypo-responsiveness and long-term allograft survival in murine models
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Jennifer Schneiderman, Longhui Qiu, Xin Yi Yeap, Xin Kang, Feibo Zheng, Junsheng Ye, Yan Xie, Jiao-Jing Wang, Yuvaraj Sambandam, James Mathew, Lin Li, Joseph Leventhal, Richard L. Edelson, and Zheng Jenny Zhang
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Medicine ,Science - Abstract
Abstract Recipients of solid organ transplantation (SOT) rely on life-long immunosuppression (IS), which is associated with significant side effects. Extracorporeal photochemotherapy (ECP) is a safe, existing cellular therapy used to treat transplant rejection by modulating the recipient’s own blood cells. We sought to induce donor-specific hypo-responsiveness of SOT recipients by infusing ECP-treated donor leukocytes prior to transplant. To this end, we utilized major histocompatibility complex mismatched rodent models of allogeneic cardiac, liver, and kidney transplantation to test this novel strategy. Leukocytes isolated from donor-matched spleens for ECP treatment (ECP-DL) were infused into transplant recipients seven days prior to SOT. Pre-transplant infusion of ECP-DL without additional IS was associated with prolonged graft survival in all models. This innovative approach promoted the production of tolerogenic dendritic cells and regulatory T-cells with subsequent inhibition of T-cell priming and differentiation, along with a significant reduction of donor-specific T-cells in the spleen and grafts of treated animals. This new application of donor-type ECP-treated leukocytes provides insight into the mechanisms behind ECP-induced immunoregulation and holds significant promise in the prevention of graft rejection and reduction in need of global immune suppressive therapy in patients following SOT.
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- 2022
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10. Genome-wide DNA methylation and gene expression patterns of androgenetic haploid tiger pufferfish (Takifugu rubripes) provide insights into haploid syndrome
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He Zhou, Qian Wang, Zi-Yu Zhou, Xin Li, Yu-Qing Sun, Gu Shan, Xin-Yi Zheng, Qi Chen, Hai-Jin Liu, Wei Wang, and Chang-Wei Shao
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Medicine ,Science - Abstract
Abstract Androgenesis is an important chromosome set manipulation technique used in sex control in aquaculture. Haploid embryos exhibit haploid syndrome with body abnormalities and even die during early embryonic development. In this study, we used whole genome bisulfite sequencing (WGBS) to investigate the genome-wide DNA methylation profiles in haploid females (1n-X) and males (1n-Y), and diploid females (2n-XX) and males (2n-XY) of tiger pufferfish (Takifugu rubripes), an economically important fish in China. A total of 96.32 Gb clean data was produced. Differentially methylated regions (DMRs) were found between haploids and diploids, which may be related to abnormal development and early embryonic death in haploids. There were 3,641 hyper-methylated differentially methylated genes (DMGs) and 2,179 hypo-methylated DMGs in haploid vs. diploid comparisons in both females and males. These DMGs were mainly related to genomic stability maintenance and cell cycle regulation. slf1, actr8, gas2, and pbrm1 genes were selected to validate the methylation sequencing. After combining the methylation data with the corresponding transcriptome data, we identified several genes, including guca2a, myoc, fezf2, rprml, telo2, s100a1, and marveld1, which exhibited differential expression levels modulated by DNA methylation. In conclusion, our study revealed different methylation and expression profiles between haploid and diploid T. rubripes for the first time. Several DMGs were identified between different ploidy levels, which may be related to haploid syndrome formation. The results expand the understanding of the effects of ploidy on the early development of teleosts and provide knowledge about target genes and networks to improve the survival rate of haploids.
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- 2022
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11. Efficacy and safety of drug-coated balloon in the treatment of acute myocardial infarction: a meta-analysis of randomized controlled trials
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Qiu-Yi Li, Mei-Ying Chang, Xin-Yi Wang, An-Lu Wang, Qi-Yu Liu, Tong Wang, Hao Xu, and Ke-Ji Chen
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Medicine ,Science - Abstract
Abstract Acute myocardial infarction (AMI) is one of the main causes of death in the world, and the incidence of AMI is increasing in the young population. Drug-coated balloon (DCB) has become an effective concept for the treatment of in-stent restenosis, small vessel disease, bifurcation lesions, high blood risk conditions, and even de novo large vessel disease. To ensure whether DCB can play an alternative role in AMI, we conducted a comprehensive meta‐analysis of randomized controlled trials (RCTs) to evaluate the efficacy and safety of DCB in the treatment of AMI. Electronic databases were searched for RCTs that compared DCB with stent for AMI. The primary outcome was major adverse cardiac events (MACEs), the secondary outcome was late lumen loss (LLL). RevMan 5.3 software and RStudio software were used for data analysis. Five RCTs involving 528 patients with 6–12 months of follow-up were included. There was no significant difference in the incidence of MACEs between DCB group and stent group (RR, 0.85; 95% CI 0.42 to 1.74; P = 0.66). Lower LLL was shown in DCB group (WMD, − 0.29; 95% CI − 0.46 to − 0.12; P
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- 2022
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12. The association between dose of hemodialysis and patients mortality in a prospective cohort study
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Liu, Shu-Xin, Wang, Zhi-Hong, Zhang, Shuang, Xiao, Jia, You, Lian-Lian, Zhang, Yu, Dong, Cui, Wang, Xue-Na, Wang, Zhen-Zhen, Wang, Sheng-Nan, Song, Jia-Ni, Zhao, Xiu-Nan, Yan, Xin-Yi, Yu, Shu-Fan, and Zhang, Yi-Nan
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- 2022
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13. Genome-wide DNA methylation and gene expression patterns of androgenetic haploid tiger pufferfish (Takifugu rubripes) provide insights into haploid syndrome
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Zhou, He, Wang, Qian, Zhou, Zi-Yu, Li, Xin, Sun, Yu-Qing, Shan, Gu, Zheng, Xin-Yi, Chen, Qi, Liu, Hai-Jin, Wang, Wei, and Shao, Chang-Wei
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- 2022
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14. Efficacy and safety of drug-coated balloon in the treatment of acute myocardial infarction: a meta-analysis of randomized controlled trials
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Li, Qiu-Yi, Chang, Mei-Ying, Wang, Xin-Yi, Wang, An-Lu, Liu, Qi-Yu, Wang, Tong, Xu, Hao, and Chen, Ke-Ji
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- 2022
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15. Generation of two multipotent mesenchymal progenitor cell lines capable of osteogenic, mature osteocyte, adipogenic, and chondrogenic differentiation
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Matthew Prideaux, Christian S. Wright, Megan L. Noonan, Xin Yi, Erica L. Clinkenbeard, Elsa Mevel, Jonathan A. Wheeler, Sharon Byers, Asiri R. Wijenayaka, Stan Gronthos, Uma Sankar, Kenneth E. White, Gerald J. Atkins, and William R. Thompson
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Medicine ,Science - Abstract
Abstract Mesenchymal progenitors differentiate into several tissues including bone, cartilage, and adipose. Targeting these cells in vivo is challenging, making mesenchymal progenitor cell lines valuable tools to study tissue development. Mesenchymal stem cells (MSCs) can be isolated from humans and animals; however, obtaining homogenous, responsive cells in a reproducible fashion is challenging. As such, we developed two mesenchymal progenitor cell (MPC) lines, MPC1 and MPC2, generated from bone marrow of male C57BL/6 mice. These cells were immortalized using the temperature sensitive large T-antigen, allowing for thermal control of proliferation and differentiation. Both MPC1 and MPC2 cells are capable of osteogenic, adipogenic, and chondrogenic differentiation. Under osteogenic conditions, both lines formed mineralized nodules, and stained for alizarin red and alkaline phosphatase, while expressing osteogenic genes including Sost, Fgf23, and Dmp1. Sost and Dmp1 mRNA levels were drastically reduced with addition of parathyroid hormone, thus recapitulating in vivo responses. MPC cells secreted intact (iFGF23) and C-terminal (cFGF23) forms of the endocrine hormone FGF23, which was upregulated by 1,25 dihydroxy vitamin D (1,25D). Both lines also rapidly entered the adipogenic lineage, expressing adipose markers after 4 days in adipogenic media. MPC cells were also capable of chondrogenic differentiation, displaying increased expression of cartilaginous genes including aggrecan, Sox9, and Comp. With the ability to differentiate into multiple mesenchymal lineages and mimic in vivo responses of key regulatory genes/proteins, MPC cells are a valuable model to study factors that regulate mesenchymal lineage allocation as well as the mechanisms that dictate transcription, protein modification, and secretion of these factors.
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- 2021
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16. Integrating gold nanoclusters, folic acid and reduced graphene oxide for nanosensing of glutathione based on 'turn-off' fluorescence
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Xin Yi Wong, Daniel Quesada-González, Sivakumar Manickam, Siu Yee New, Kasturi Muthoosamy, and Arben Merkoçi
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Medicine ,Science - Abstract
Abstract Glutathione (GSH) is a useful biomarker in the development, diagnosis and treatment of cancer. However, most of the reported GSH biosensors are expensive, time-consuming and often require complex sample treatment, which limit its biological applications. Herein, a nanobiosensor for the detection of GSH using folic acid-functionalized reduced graphene oxide-modified BSA gold nanoclusters (FA-rGO-BSA/AuNCs) based on the fluorescence quenching interactions is presented. Firstly, a facile and optimized protocol for the fabrication of BSA/AuNCs is developed. Functionalization of rGO with folic acid is performed using EDC/NHS cross-linking reagents, and their interaction after loading with BSA/AuNCs is demonstrated. The formation of FA-rGO, BSA/AuNCs and FA-rGO-BSA/AuNCs are confirmed by the state-of-art characterization techniques. Finally, a fluorescence turn-off sensing strategy is developed using the as-synthesized FA-rGO-BSA/AuNCs for the detection of GSH. The nanobiosensor revealed an excellent sensing performance for the detection of GSH with high sensitivity and desirable selectivity over other potential interfering species. The fluorescence quenching is linearly proportional to the concentration of GSH between 0 and 1.75 µM, with a limit of detection of 0.1 µM under the physiological pH conditions (pH 7.4). Such a sensitive nanobiosensor paves the way to fabricate a “turn-on” or “turn-off” fluorescent sensor for important biomarkers in cancer cells, presenting potential nanotheranostic applications in biological detection and clinical diagnosis.
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- 2021
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17. CT image segmentation for inflamed and fibrotic lungs using a multi-resolution convolutional neural network
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Gerard, Sarah E., Herrmann, Jacob, Xin, Yi, Martin, Kevin T., Rezoagli, Emanuele, Ippolito, Davide, Bellani, Giacomo, Cereda, Maurizio, Guo, Junfeng, Hoffman, Eric A., Kaczka, David W., and Reinhardt, Joseph M.
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- 2021
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18. Integrating gold nanoclusters, folic acid and reduced graphene oxide for nanosensing of glutathione based on “turn-off” fluorescence
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Wong, Xin Yi, Quesada-González, Daniel, Manickam, Sivakumar, New, Siu Yee, Muthoosamy, Kasturi, and Merkoçi, Arben
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- 2021
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19. MiR-155/GSK-3β mediates anti-inflammatory effect of Chikusetsusaponin IVa by inhibiting NF-κB signaling pathway in LPS-induced RAW264.7 cell
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Xin, Yi, Yuan, Qin, Liu, Chaoqi, Zhang, Changcheng, and Yuan, Ding
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- 2020
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20. Chronic lymphocytic leukemia-associated paraneoplastic pemphigus: potential cause and therapeutic strategies
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Cao, Lei, Wang, Fei, Du, Xin-Yi, Zhu, Hua-Yuan, Wang, Li, Xu, Wei, Li, Jian-Yong, and Fan, Lei
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- 2020
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21. Transcriptomics-based investigation of manganese dioxide nanoparticle toxicity in rats’ choroid plexus
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Memg, Chun-Yan, primary, Ma, Xin-Yi, additional, Xu, Ming-Yan, additional, Pei, Sheng-Fei, additional, Liu, Yang, additional, Hao, Zhuo-Lu, additional, Li, Qing-Zhao, additional, and Feng, Fu-Min, additional
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- 2023
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22. A U-shaped Association of Breastfeeding Duration with Cognitive Impairment in Chinese Postmenopausal Women
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Li, Fu-Dong, Lin, Jun-Fen, Ying, Xu-Hua, Qiu, Yin-Wei, Li, Song-Tao, Zhai, Yu-Jia, Zhang, Tao, Wang, Xin-Yi, Gu, Xue, Wu, Meng-Na, and He, Fan
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- 2020
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23. Genome-wide comparative analysis of H3K4me3 profiles between diploid and allotetraploid cotton to refine genome annotation
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Qi You, Xin Yi, Kang Zhang, Chunchao Wang, Xuelian Ma, Xueyan Zhang, Wenying Xu, Fuguang Li, and Zhen Su
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Medicine ,Science - Abstract
Abstract Polyploidy is a common evolutionary occurrence in plants. Recently, published genomes of allotetraploid G. hirsutum and its donors G. arboreum and G. raimondii make cotton an accessible polyploid model. This study used chromatin immunoprecipitation with high-throughput sequencing (ChIP-Seq) to investigate the genome-wide distribution of H3K4me3 in G. arboreum and G. hirsutum, and explore the conservation and variation of genome structures between diploid and allotetraploid cotton. Our results showed that H3K4me3 modifications were associated with active transcription in both cottons. The H3K4me3 histone markers appeared mainly in genic regions and were enriched around the transcription start sites (TSSs) of genes. We integrated the ChIP-seq data of H3K4me3 with RNA-seq and ESTs data to refine the genic structure annotation. There were 6,773 and 12,773 new transcripts discovered in G. arboreum and G. hirsutum, respectively. Furthermore, co-expression networks were linked with histone modification and modularized in an attempt to explain differential H3K4me3 enrichment correlated with changes in gene transcription during cotton development and evolution. Taken together, we have combined epigenomic and transcriptomic datasets to systematically discover functional genes and compare them between G. arboreum and G. hirsutum, which may be beneficial for studying diploid and allotetraploid plants with large genomes and complicated evolution.
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- 2017
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24. Palladium nanoparticles entrapped in a self-supporting nanoporous gold wire as sensitive dopamine biosensor
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Xin Yi, Yuxuan Wu, Guoxin Tan, Peng Yu, Lei Zhou, Zhengnan Zhou, Junqi Chen, Zhengao Wang, Jinshan Pang, and Chengyun Ning
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Medicine ,Science - Abstract
Abstract Traced dopamine (DA) detection is critical for the early diagnosis and prevention of some diseases such as Parkinson’s, Alzheimer and schizophrenia. In this research, a novel self-supporting three dimensional (3D) bicontinuous nanoporous electrochemical biosensor was developed for the detection of dopamine by Differential Pulse Voltammetry (DPV). This biosensor was fabricated by electrodepositing palladium nanoparticles (Pd) onto self-supporting nanoporous gold (NPG) wire. Because of the synergistic effects of the excellent catalytic activity of Pd and novel structure of NPG wire, the palladium nanoparticles decorated NPG (Pd/NPG) biosensor possess tremendous superiority in the detection of DA. The Pd/NPG wire biosensor exhibited high sensitivity of 1.19 μA μΜ−1, broad detection range of 1–220 μM and low detection limit up to 1 μM. Besides, the proposed dopamine biosensor possessed good stability, reproducibility, reusability and selectivity. The response currents of detection in the fetal bovine serum were also close to the standard solutions. Therefore the Pd/NPG wire biosensor is promising to been used in clinic.
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- 2017
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25. Noninvasive prenatal diagnosis of 21-Hydroxylase deficiency using target capture sequencing of maternal plasma DNA
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Dingyuan Ma, Yuan Yuan, Chunyu Luo, Yaoshen Wang, Tao Jiang, Fengyu Guo, Jingjing Zhang, Chao Chen, Yun Sun, Jian Cheng, Ping Hu, Jian Wang, Huanming Yang, Xin Yi, Wei Wang, Asan, and Zhengfeng Xu
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Medicine ,Science - Abstract
Abstract Here, we aimed to validate a noninvasive method using capture sequencing for prenatal diagnosis of congenital adrenal hyperplasia due to 21-Hydroxylase deficiency (21-OHD). Noninvasive prenatal diagnosis (NIPD) of 21-OHD was based on 14 plasma samples collected from 12 families, including four plasma sample collected during the first trimester. Targeted capture sequencing was performed using genomic DNA from the parents and child trios to determine the pathogenic and wild-type alleles associated with the haplotypes. Maternal plasma DNA was also sequenced to determine the fetal inheritance of the allele using hidden Markov model-based haplotype linkage analysis. The effect of fetal DNA fraction and sequencing depth on the accuracy of NIPD was investigated. The lower limit of fetal DNA fraction was 2% and the threshold mean sequence depth was 38, suggesting potential advantage if used in early gestation. The CYP21A2 genotype of the fetus was accurately determined in all the 14 plasma samples as early as day 1 and 8 weeks of gestation. Results suggest the accuracy and feasibility of NIPD of 21-OHD using a small target capture region with a low threshold for fetal DNA fraction and sequence depth. Our method is cost-effective and suggests diagnostic applications in clinical practice.
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- 2017
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26. Bilirubin augments Ca2+ load of developing bushy neurons by targeting specific subtype of voltage-gated calcium channels
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Min Liang, Xin-Lu Yin, Hai-Bo Shi, Chun-Yan Li, Xin-Yi Li, Ning-Ying Song, Hao-Song Shi, Yi Zhao, Lu-Yang Wang, and Shan-Kai Yin
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Medicine ,Science - Abstract
Abstract Neonatal brain is particularly vulnerable to pathological levels of bilirubin which elevates and overloads intracellular Ca2+, leading to neurotoxicity. However, how voltage-gated calcium channels (VGCCs) are functionally involved in excess calcium influx remains unknown. By performing voltage-clamp recordings from bushy cells in the ventral cochlear nucleus (VCN) in postnatal rat pups (P4-17), we found the total calcium current density was more than doubled over P4-17, but the relative weight of VGCC subtypes changed dramatically, being relatively equal among T, L, N, P/Q and R-type at P4-6 to predominantly L, N, R over T and P/Q at P15-17. Surprisingly, acute administration of bilirubin augmented the VGCC currents specifically mediated by high voltage-activated (HVA) P/Q-type calcium currents. This augment was attenuated by intracellular loading of Ca2+ buffer EGTA or calmodulin inhibitory peptide. Our findings indicate that acute exposure to bilirubin increases VGCC currents, primarily by targeting P/Q-type calcium channels via Ca2+ and calmodulin dependent mechanisms to overwhelm neurons with excessive Ca2+. Since P/Q-subtype calcium channels are more prominent in neonatal neurons (e.g. P4-6) than later stages, we suggest this subtype-specific enhancement of P/Q-type Ca2+ currents likely contributes to the early neuronal vulnerability to hyperbilirubinemia in auditory and other brain regions.
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- 2017
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27. Differential expression of chemosensory-protein genes in midguts in response to diet of Spodoptera litura
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Xin Yi, Jiangwei Qi, Xiaofan Zhou, Mei Ying Hu, and Guo Hua Zhong
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Medicine ,Science - Abstract
Abstract While it has been well characterized that chemosensory receptors in guts of mammals have great influence on food preference, much remains elusive in insects. Insect chemosensory proteins (CSPs) are soluble proteins that could deliver chemicals to olfactory and gustatory receptors. Recent studies have identified a number of CSPs expressed in midgut in Lepidoptera insects, which started to reveal their roles in chemical recognition and stimulating appetite in midgut. In this study, we examined expression patterns in midgut of 21 Spodoptera litura CSPs (SlitCSPs) characterized from a previously reported transcriptome, and three CSPs were identified to be expressed highly in midgut. The orthologous relationships between midgut expressed CSPs in S. litura and those in Bombyx mori and Plutella xylostella also suggest a conserved pattern of CSP expression in midgut. We further demonstrated that the expression of midgut-CSPs may change in response to different host plants, and SlitCSPs could bind typical chemicals from host plant in vitro. Overall, our results suggested midgut expressed SlitCSPs may have functional roles, likely contributing to specialization and adaption to different ecosystems. Better knowledge of this critical component of the chemsensation signaling pathways in midguts may improve our understanding of food preference processes in a new perspective.
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- 2017
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28. Functional Evaluation of Two Corneal Endothelial Cell-Based Therapies: Tissue-Engineered Construct and Cell Injection
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Peh, Gary S. L., Ong, Hon Shing, Adnan, Khadijah, Ang, Heng-Pei, Lwin, Chan N., Seah, Xin-Yi, Lin, Shu-Jun, and Mehta, Jodhbir S.
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- 2019
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29. Measurement of Regional 2D Gas Transport Efficiency in Rabbit Lung Using Hyperpolarized 129Xe MRI
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Ruppert, Kai, Xin, Yi, Hamedani, Hooman, Amzajerdian, Faraz, Loza, Luis, Achekzai, Tahmina, Duncan, Ian F., Profka, Harrilla, Siddiqui, Sarmad, Pourfathi, Mehrdad, Sertic, Federico, Cereda, Maurizio F., Kadlecek, Stephen, and Rizi, Rahim R.
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- 2019
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30. Chronic lymphocytic leukemia-associated paraneoplastic pemphigus: potential cause and therapeutic strategies
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Wei Xu, Lei Cao, Li Wang, Xin-Yi Du, Jianyong Li, Lei Fan, Fei Wang, and Hua-Yuan Zhu
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Adult ,Male ,Oncology ,inorganic chemicals ,medicine.medical_specialty ,Paraneoplastic Syndromes ,Chronic lymphocytic leukemia ,Secondary infection ,lcsh:Medicine ,Antineoplastic Agents ,030207 dermatology & venereal diseases ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Prednisone ,Internal medicine ,hemic and lymphatic diseases ,medicine ,Humans ,heterocyclic compounds ,lcsh:Science ,Retrospective Studies ,Aged, 80 and over ,Multidisciplinary ,business.industry ,lcsh:R ,Middle Aged ,Prognosis ,medicine.disease ,Leukemia, Lymphocytic, Chronic, B-Cell ,Fludarabine ,Regimen ,enzymes and coenzymes (carbohydrates) ,Paraneoplastic pemphigus ,chemistry ,030220 oncology & carcinogenesis ,Ibrutinib ,Female ,Rituximab ,lcsh:Q ,business ,Pemphigus ,Vidarabine ,medicine.drug - Abstract
Paraneoplastic pemphigus (PNP) is a severe autoimmune syndrome commonly triggered by neoplasms. The prognosis of CLL-associated PNP is dismal due to its refractory course and secondary infection and no standard treatment was recommended. We retrospectively reported six CLL with PNP cases from 842 cases of CLL including diagnosis, treatment and prognosis. The median time between the initial of CLL to PNP was 36 months while the median overall survival from the diagnosis of PNP was 26 months. And three cases died of lung infection while 5 developed pulmonary symptoms. And 5 cases received fludarabine-based chemotherapy before developing PNP, which suggesting fludarabine was one of potential causes of PNP. For the treatment, five patients were rescued by combined regimens including rituximab, methylprednisolone, immunoglobulin, fresh frozen plasma and the last received ibrutinib combined with short-term prednisone. Fludarabine-based regimen may be one of the potential causes of PNP. The combined regimen might shed a new light, while ibrutinib is a promising drug for CLL with PNP, but needs much more evidence. PNP should be carefully treated to guide early diagnosis and intervention for a better prognosis.
- Published
- 2020
31. Pre-transplant infusion of donor leukocytes treated with extracorporeal photochemotherapy induces immune hypo-responsiveness and long-term allograft survival in murine models
- Author
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Jennifer Schneiderman, Longhui Qiu, Xin Yi Yeap, Xin Kang, Feibo Zheng, Junsheng Ye, Yan Xie, Jiao-Jing Wang, Yuvaraj Sambandam, James Mathew, Lin Li, Joseph Leventhal, Richard L. Edelson, and Zheng Jenny Zhang
- Subjects
Homologous ,Graft Rejection ,Transplantation ,Kidney Disease ,Multidisciplinary ,5.2 Cellular and gene therapies ,Animal ,T-Lymphocytes ,Graft Survival ,Evaluation of treatments and therapeutic interventions ,Organ Transplantation ,Allografts ,Regulatory ,T-Lymphocytes, Regulatory ,Disease Models, Animal ,Mice ,6.1 Pharmaceuticals ,Photopheresis ,Disease Models ,Animals ,Humans ,Transplantation, Homologous ,Development of treatments and therapeutic interventions - Abstract
Recipients of solid organ transplantation (SOT) rely on life-long immunosuppression (IS), which is associated with significant side effects. Extracorporeal photochemotherapy (ECP) is a safe, existing cellular therapy used to treat transplant rejection by modulating the recipient’s own blood cells. We sought to induce donor-specific hypo-responsiveness of SOT recipients by infusing ECP-treated donor leukocytes prior to transplant. To this end, we utilized major histocompatibility complex mismatched rodent models of allogeneic cardiac, liver, and kidney transplantation to test this novel strategy. Leukocytes isolated from donor-matched spleens for ECP treatment (ECP-DL) were infused into transplant recipients seven days prior to SOT. Pre-transplant infusion of ECP-DL without additional IS was associated with prolonged graft survival in all models. This innovative approach promoted the production of tolerogenic dendritic cells and regulatory T-cells with subsequent inhibition of T-cell priming and differentiation, along with a significant reduction of donor-specific T-cells in the spleen and grafts of treated animals. This new application of donor-type ECP-treated leukocytes provides insight into the mechanisms behind ECP-induced immunoregulation and holds significant promise in the prevention of graft rejection and reduction in need of global immune suppressive therapy in patients following SOT.
- Published
- 2021
32. Generation of two multipotent mesenchymal progenitor cell lines capable of osteogenic, mature osteocyte, adipogenic, and chondrogenic differentiation
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Xin Yi, Matthew Prideaux, Erica L. Clinkenbeard, Asiri R. Wijenayaka, Sharon Byers, Uma Sankar, Gerald J. Atkins, Kenneth E. White, Jonathan A. Wheeler, Megan L. Noonan, William R. Thompson, Stan Gronthos, Christian S. Wright, and Elsa Mevel
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Male ,Science ,Cell Culture Techniques ,Adipose tissue ,Hormone receptors ,Biology ,Osteocytes ,Article ,Cell Line ,Immunophenotyping ,Mice ,Chondrocytes ,Adipocytes ,medicine ,Animals ,Cell Lineage ,RNA, Messenger ,Progenitor cell ,Aggrecan ,Cell Proliferation ,Multidisciplinary ,Mesenchymal stem cell ,Cell Differentiation ,Mesenchymal Stem Cells ,Chondrogenesis ,Cell biology ,Mice, Inbred C57BL ,Fibroblast Growth Factor-23 ,medicine.anatomical_structure ,Adipogenesis ,Osteocyte ,Medicine ,Cell culture ,Bone marrow - Abstract
Mesenchymal progenitors differentiate into several tissues including bone, cartilage, and adipose. Targeting these cells in vivo is challenging, making mesenchymal progenitor cell lines valuable tools to study tissue development. Mesenchymal stem cells (MSCs) can be isolated from humans and animals; however, obtaining homogenous, responsive cells in a reproducible fashion is challenging. As such, we developed two mesenchymal progenitor cell (MPC) lines, MPC1 and MPC2, generated from bone marrow of male C57BL/6 mice. These cells were immortalized using the temperature sensitive large T-antigen, allowing for thermal control of proliferation and differentiation. Both MPC1 and MPC2 cells are capable of osteogenic, adipogenic, and chondrogenic differentiation. Under osteogenic conditions, both lines formed mineralized nodules, and stained for alizarin red and alkaline phosphatase, while expressing osteogenic genes including Sost, Fgf23, and Dmp1. Sost and Dmp1 mRNA levels were drastically reduced with addition of parathyroid hormone, thus recapitulating in vivo responses. MPC cells secreted intact (iFGF23) and C-terminal (cFGF23) forms of the endocrine hormone FGF23, which was upregulated by 1,25 dihydroxy vitamin D (1,25D). Both lines also rapidly entered the adipogenic lineage, expressing adipose markers after 4 days in adipogenic media. MPC cells were also capable of chondrogenic differentiation, displaying increased expression of cartilaginous genes including aggrecan, Sox9, and Comp. With the ability to differentiate into multiple mesenchymal lineages and mimic in vivo responses of key regulatory genes/proteins, MPC cells are a valuable model to study factors that regulate mesenchymal lineage allocation as well as the mechanisms that dictate transcription, protein modification, and secretion of these factors.
- Published
- 2021
33. Second-generation cryoballoon versus contact force radiofrequency ablation for atrial fibrillation: an updated meta-analysis of evidence from randomized controlled trials
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Xin-Yi Li, Qian Chen, Zhengtian Lv, Yang Lou, Wei Mao, Chenxia Wu, and Xinbin Zhou
- Subjects
Male ,Risk ,medicine.medical_specialty ,Radiofrequency ablation ,Science ,medicine.medical_treatment ,Catheter ablation ,Cochrane Library ,Cryosurgery ,Article ,law.invention ,Randomized controlled trial ,law ,Internal medicine ,Atrial Fibrillation ,Oculomotor Nerve Diseases ,medicine ,Humans ,Randomized Controlled Trials as Topic ,Cardiac device therapy ,Radiofrequency Ablation ,Multidisciplinary ,business.industry ,Atrial fibrillation ,Middle Aged ,Ablation ,medicine.disease ,Confidence interval ,Phrenic Nerve ,Treatment Outcome ,Relative risk ,Cardiology ,Medicine ,Female ,business ,Interventional cardiology - Abstract
Catheter ablation has been recommended for patients with symptomatic atrial fibrillation (AF), with pulmonary vein isolation being the cornerstone of the ablation procedure. Newly developed technologies, such as cryoballoon ablation with a second-generation cryoballoon (CB2) and the contact force radiofrequency (CF-RF) ablation, have been introduced in recent years to overcome the shortcomings of the widely used RF ablation approach. However, high-quality results comparing CB2 and CF-RF remain controversial. Thus, we conducted this meta-analysis to assess the efficacy and safety between CB2 and CF-RF using evidence from randomized controlled trials (RCTs). Databases including Embase, PubMed, the Cochrane Library, and ClinicalTrials.gov were systematically searched from their date of inception to January 2021. Only RCTs that met the inclusion criteria were included for analysis. The primary outcome of interest was freedom from atrial tachyarrhythmia (AT) during follow-up. Secondary outcomes included procedure-related complications, procedure time and fluoroscopy time. Six RCTs with a total of 987 patients were finally enrolled. No significant differences were found between CB2 and CF-RF in terms of freedom from AT (relative risk [RR] = 1.03, 95% confidence interval [CI] 0.92–1.14, p = 0.616) or total procedural-related complications (RR = 1.25, 95% CI 0.69–2.27, p = 0.457). CB2 treatment was associated with a significantly higher risk of phrenic nerve palsy (PNP) than CF-RF (RR = 4.93, 95% CI 1.12–21.73, p = 0.035). The occurrences of pericardial effusion/tamponade and vascular complications were comparable between the CB2 and CF-RF treatments (RR = 0.41, p = 0.398; RR = 0.82, p = 0.632). In addition, CB2 treatment had a significantly shorter procedure time than CF-RF (weighted mean difference [WMD] = − 20.75 min, 95% CI − 25.44 ~ − 16.05 min, P p = 0.179). CB2 and CF-RF treatment are comparable for AF patients regarding freedom from AT and procedure-related complications. Compared to CF-RF, CB2 treatment was associated with a shorter procedure time but a higher incidence of PNP. Further large-scale studies are warranted to compare these two techniques and provide an up-to-date recommendation.
- Published
- 2021
34. The transcutaneous electromyography recording method for intraoperative neuromonitoring of recurrent laryngeal nerve during minimally invasive parathyroidectomy
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Bin Han, Qing-Zhuang Liang, Feng-Yu Chiang, Peng Li, Xin Yi, Wei Wei, and Dong-Lai Wang
- Subjects
Adult ,Male ,medicine.medical_specialty ,Cord ,lcsh:Medicine ,Parathyroid diseases ,Electromyography ,Article ,03 medical and health sciences ,Intraoperative Period ,0302 clinical medicine ,medicine ,Recurrent laryngeal nerve ,Humans ,Minimally Invasive Surgical Procedures ,Action potential generation ,030223 otorhinolaryngology ,lcsh:Science ,Aged ,Parathyroidectomy ,Multidisciplinary ,medicine.diagnostic_test ,business.industry ,Recurrent Laryngeal Nerve ,Thyroid ,lcsh:R ,Middle Aged ,medicine.disease ,Thyroid cartilage ,Vagus nerve ,Surgery ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,lcsh:Q ,Female ,business ,Minimally invasive parathyroidectomy ,Primary hyperparathyroidism - Abstract
Intraoperative neuromonitoring (IONM) facilitates recurrent laryngeal nerve (RLN) protection in thyroid and parathyroid surgeries. This study aimed to investigate a novel transcutaneous electromyography (EMG) recording method for IONM of the RLN during minimally invasive parathyroidectomy (MIP). Twenty patients with primary hyperparathyroidism undergoing MIP were enrolled. Two paired needle electrodes were percutaneously inserted into the bilateral laminas of thyroid cartilage for monitoring the vagus nerve and RLN. A standardized IONM procedure (V1-R1-R2-V2 signals) was strictly followed, and the RLN was routinely located and mapped. Pre- and postoperative laryngofiberoscopy was performed to confirm vocal cord function. The proposed technique was successfully used in all patients, and typical EMG signals were effectively detected. No significant change in EMG signals before and after tumor resection was noted, and a normal vocal cord movement was ensured in all patients with postoperative laryngofiberoscopy. IONM helped localize the position of the RLN and facilitated the safe resection of the parathyroid tumor during MIP. The novel transcutaneous EMG recording method proposed in this study was feasible, convenient, reliable, and inexpensive.
- Published
- 2019
35. Adipose mesenchymal stem cells from osteoporotic donors preserve functionality and modulate systemic inflammatory microenvironment in osteoporotic cytotherapy
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Pan Zhao, Chen-Xi Zheng, Kun Xuan, Tao He, Bing-Dong Sui, Chenghu Hu, Yan Jin, Xin-Yi Zhang, Nu Liu, and Ji Chen
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0301 basic medicine ,Ovariectomy ,T-Lymphocytes ,Energy metabolism ,Cell- and Tissue-Based Therapy ,lcsh:Medicine ,Adipose tissue ,Apoptosis ,Defence system ,Mesenchymal Stem Cell Transplantation ,Article ,03 medical and health sciences ,Mice ,Medicine ,Animals ,Humans ,lcsh:Science ,Inflammation ,Multidisciplinary ,business.industry ,lcsh:R ,Mesenchymal stem cell ,Mesenchymal Stem Cells ,030104 developmental biology ,medicine.anatomical_structure ,Cellular Microenvironment ,Ovariectomized rat ,Cancer research ,Osteoporosis ,lcsh:Q ,Bone marrow ,business ,Energy Metabolism ,Homeostasis - Abstract
Maintenance of bone homeostasis against diseased microenvironments remains as a major challenge. Recently, mesenchymal stem cells (MSCs) have been unravelled as potent microenvironmental modulators, the systemic infusion of which in cytotherapy can prevent or rescue extensive bone loss via anti-inflammation. However, MSCs also accept microenvironmental regulations; particularly, MSCs from bone marrow (BMMSCs) are prone to pathological microenvironmental factors of bone. In this study, we discovered that BMMSCs from osteoporotic donors of ovariectomized (OVX) mice lost their anti-inflammatory capability and failed to prevent bone loss when infused back into OVX recipients. Nevertheless, MSCs from adipose tissues (ADMSCs) preserved their anti-inflammatory capacity, despite diseased microenvironments of OVX donors, and continued to show protective effects on bone in OVX recipients. In the cellular level, the anti-inflammatory superiority of osteoporotic donor-derived ADMSCs over BMMSCs existed in their distinctive capability to induce T-cell apoptosis, which was molecularly attributed to retained expression levels of critical immunomodulatory genes. Furthermore, these functional discrepancies of BMMSCs and ADMSCs were due to differential stemness, energy metabolism and anti-oxidative defence system, underlying general disparity in their cellular states. Collectively, our findings optimize osteoporotic cytotherapy by using ADMSCs in resistance to and in modulation of diseased microenvironments.
- Published
- 2018
36. Transcriptome analysis of Spodoptera frugiperda Sf9 cells reveals putative apoptosis-related genes and a preliminary apoptosis mechanism induced by azadirachtin
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Veeran Sethuraman, Guohua Zhong, Xin Yi, Benshui Shu, Gaofeng Cui, and Jingjing Zhang
- Subjects
Limonins ,0106 biological sciences ,0301 basic medicine ,Insecticides ,viruses ,Blotting, Western ,lcsh:Medicine ,Apoptosis ,Genes, Insect ,Sf9 ,Spodoptera ,Biology ,01 natural sciences ,Article ,Cell Line ,Transcriptome ,03 medical and health sciences ,Gene expression ,Sf9 Cells ,Animals ,KEGG ,lcsh:Science ,Gene ,Illumina dye sequencing ,Multidisciplinary ,Gene Expression Profiling ,lcsh:R ,fungi ,biology.organism_classification ,Molecular biology ,Up-Regulation ,Gene expression profiling ,010602 entomology ,030104 developmental biology ,Caspases ,lcsh:Q ,Apoptosis Regulatory Proteins - Abstract
As an important botanical pesticide, azadirachtin demonstrates broad insecticidal activity against many agricultural pests. The results of a previous study indicated the toxicity and apoptosis induction of azadirachtin in Spodoptera frugiperda Sf9 cells. However, the lack of genomic data has hindered a deeper investigation of apoptosis in Sf9 cells at a molecular level. In the present study, the complete transcriptome data for Sf9 cell line was accomplished using Illumina sequencing technology, and 97 putative apoptosis-related genes were identified through BLAST and KEGG orthologue annotations. Fragments of potential candidate apoptosis-related genes were cloned, and the mRNA expression patterns of ten identified genes regulated by azadirachtin were examined using qRT-PCR. Furthermore, Western blot analysis showed that six putative apoptosis-related proteins were upregulated after being treated with azadirachtin while the protein Bcl-2 were downregulated. These data suggested that both intrinsic and extrinsic apoptotic signal pathways comprising the identified potential apoptosis-related genes were potentially active in S. frugiperda. In addition, the preliminary results revealed that caspase-dependent or caspase-independent apoptotic pathways could function in azadirachtin-induced apoptosis in Sf9 cells.
- Published
- 2017
37. Regulatory Compliant Tissue-Engineered Human Corneal Endothelial Grafts Restore Corneal Function of Rabbits with Bullous Keratopathy
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Gary S L Peh, Donald T.H. Tan, Heng-Pei Ang, Chan N. Lwin, Yu-Chi Liu, Xin-Yi Seah, Benjamin L. George, Khadijah Adnan, Shu-Jun Lin, Maninder Bhogal, Jodhbir S. Mehta, and School of Materials Science & Engineering
- Subjects
0301 basic medicine ,Male ,medicine.medical_treatment ,Cell Culture Techniques ,lcsh:Medicine ,Corneal Diseases ,Corneal Transplantation ,0302 clinical medicine ,Tissue engineering ,Tissue-engineered Endothelial Keratoplasty ,Endothelial dysfunction ,lcsh:Science ,Child ,Multidisciplinary ,Endothelium, Corneal ,Extracellular Matrix ,Engineering::Materials [DRNTU] ,medicine.anatomical_structure ,Child, Preschool ,Female ,Rabbits ,Adult ,medicine.medical_specialty ,Corneal endothelium ,Endothelium ,Adolescent ,Corneal Stroma ,Article ,03 medical and health sciences ,Ophthalmology ,medicine ,Animals ,Humans ,Corneal transplantation ,Cryopreservation ,Tissue Engineering ,business.industry ,lcsh:R ,medicine.disease ,eye diseases ,Surgery ,Transplantation ,Disease Models, Animal ,030104 developmental biology ,030221 ophthalmology & optometry ,Bullous keratopathy ,lcsh:Q ,Corneal Endothelial ,sense organs ,business - Abstract
Corneal transplantation is the only treatment available to restore vision for individuals with blindness due to corneal endothelial dysfunction. However, severe shortage of available donor corneas remains a global challenge. Functional regulatory compliant tissue-engineered corneal endothelial graft substitute can alleviate this reliance on cadaveric corneal graft material. Here, isolated primary human corneal endothelial cells (CEnCs) propagated using a dual media approach refined towards regulatory compliance showed expression of markers indicative of the human corneal endothelium, and can be tissue-engineered onto thin corneal stromal carriers. Both cellular function and clinical adaptability was demonstrated in a pre-clinical rabbit model of bullous keratopathy using a tissue-engineered endothelial keratoplasty (TE-EK) approach, adapted from routine endothelial keratoplasty procedure for corneal transplantation in human patients. Cornea thickness of rabbits receiving TE-EK graft gradually reduced over the first two weeks, and completely recovered to a thickness of approximately 400 µm by the third week of transplantation, whereas corneas of control rabbits remained significantly thicker over 1,000 µm (p
- Published
- 2017
38. Palladium nanoparticles entrapped in a self-supporting nanoporous gold wire as sensitive dopamine biosensor
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Zhengao Wang, Chengyun Ning, Xin Yi, Guoxin Tan, Lei Zhou, Zhengnan Zhou, Junqi Chen, Yuxuan Wu, Peng Yu, and Jinshan Pang
- Subjects
0301 basic medicine ,Materials science ,Science ,Dopamine ,Metal Nanoparticles ,Nanotechnology ,Biosensing Techniques ,macromolecular substances ,02 engineering and technology ,Article ,Nanopores ,03 medical and health sciences ,Limit of Detection ,medicine ,Electrochemical biosensor ,Detection limit ,Multidisciplinary ,Nanowires ,Nanoporous ,technology, industry, and agriculture ,Palladium nanoparticles ,021001 nanoscience & nanotechnology ,030104 developmental biology ,Medicine ,Gold ,Differential pulse voltammetry ,0210 nano-technology ,Selectivity ,Biosensor ,Palladium ,medicine.drug - Abstract
Traced dopamine (DA) detection is critical for the early diagnosis and prevention of some diseases such as Parkinson’s, Alzheimer and schizophrenia. In this research, a novel self-supporting three dimensional (3D) bicontinuous nanoporous electrochemical biosensor was developed for the detection of dopamine by Differential Pulse Voltammetry (DPV). This biosensor was fabricated by electrodepositing palladium nanoparticles (Pd) onto self-supporting nanoporous gold (NPG) wire. Because of the synergistic effects of the excellent catalytic activity of Pd and novel structure of NPG wire, the palladium nanoparticles decorated NPG (Pd/NPG) biosensor possess tremendous superiority in the detection of DA. The Pd/NPG wire biosensor exhibited high sensitivity of 1.19 μA μΜ−1, broad detection range of 1–220 μM and low detection limit up to 1 μM. Besides, the proposed dopamine biosensor possessed good stability, reproducibility, reusability and selectivity. The response currents of detection in the fetal bovine serum were also close to the standard solutions. Therefore the Pd/NPG wire biosensor is promising to been used in clinic.
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- 2017
39. Genome-wide comparative analysis of H3K4me3 profiles between diploid and allotetraploid cotton to refine genome annotation
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Xin Yi, Xueyan Zhang, Zhen Su, Kang Zhang, Fuguang Li, Chunchao Wang, Qi You, Wenying Xu, and Xuelian Ma
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0301 basic medicine ,Epigenomics ,Chromatin Immunoprecipitation ,DNA, Plant ,Transcription, Genetic ,Science ,Biology ,Genome ,Article ,Epigenesis, Genetic ,Histones ,Polyploidy ,03 medical and health sciences ,Polyploid ,Gene Regulatory Networks ,Gene ,Genetics ,Expressed Sequence Tags ,Expressed sequence tag ,Gossypium ,Multidisciplinary ,Sequence Analysis, RNA ,Gene Expression Profiling ,food and beverages ,High-Throughput Nucleotide Sequencing ,Molecular Sequence Annotation ,Genome project ,Sequence Analysis, DNA ,030104 developmental biology ,Medicine ,Ploidy ,Transcription Initiation Site ,Chromatin immunoprecipitation - Abstract
Polyploidy is a common evolutionary occurrence in plants. Recently, published genomes of allotetraploid G. hirsutum and its donors G. arboreum and G. raimondii make cotton an accessible polyploid model. This study used chromatin immunoprecipitation with high-throughput sequencing (ChIP-Seq) to investigate the genome-wide distribution of H3K4me3 in G. arboreum and G. hirsutum, and explore the conservation and variation of genome structures between diploid and allotetraploid cotton. Our results showed that H3K4me3 modifications were associated with active transcription in both cottons. The H3K4me3 histone markers appeared mainly in genic regions and were enriched around the transcription start sites (TSSs) of genes. We integrated the ChIP-seq data of H3K4me3 with RNA-seq and ESTs data to refine the genic structure annotation. There were 6,773 and 12,773 new transcripts discovered in G. arboreum and G. hirsutum, respectively. Furthermore, co-expression networks were linked with histone modification and modularized in an attempt to explain differential H3K4me3 enrichment correlated with changes in gene transcription during cotton development and evolution. Taken together, we have combined epigenomic and transcriptomic datasets to systematically discover functional genes and compare them between G. arboreum and G. hirsutum, which may be beneficial for studying diploid and allotetraploid plants with large genomes and complicated evolution.
- Published
- 2017
40. Gene-based genome-wide association study identified 19p13.3 for lean body mass
- Author
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Xiao Li, Xin-Yi You, Yu-Fang Pei, Wei-Wen Kong, Yingying Han, Hong-Wen Deng, Lei Zhang, Lu Liu, Wen Zhao, Hui Shen, An-Ping Feng, Shu Ran, Yonghong Zhang, Qing Tian, and Yong Lin
- Subjects
Adult ,Male ,0301 basic medicine ,Genotype ,Genome-wide association study ,Locus (genetics) ,030105 genetics & heredity ,Biology ,Body weight ,Polymorphism, Single Nucleotide ,Article ,03 medical and health sciences ,Human health ,Thinness ,Humans ,Gene ,Aged ,Genetics ,Single locus ,Multidisciplinary ,Chromosome Mapping ,Middle Aged ,Phenotype ,030104 developmental biology ,Body Composition ,Lean body mass ,Trait ,Female ,Chromosomes, Human, Pair 19 ,Genome-Wide Association Study - Abstract
Lean body mass (LBM) is a complex trait for human health. To identify genomic loci underlying LBM, we performed a gene-based genome-wide association study of lean mass index (LMI) in 1000 unrelated Caucasian subjects, and replicated in 2283 unrelated Caucasians subjects. Gene-based association analyses highlighted the significant associations of three genes UQCR, TCF3 and MBD3 in one single locus 19p13.3 (discovery p = 6.10 × 10−5, 1.65 × 10−4 and 1.10 × 10−4; replication p = 2.21 × 10−3, 1.84 × 10−3 and 6.95 × 10−3; combined p = 2.26 × 10−6, 4.86 × 10−6 and 1.15 × 10−5, respectively). These results, together with the known functional relevance of the three genes to LMI, suggested that the 19p13.3 region containing UQCR, TCF3 and MBD3 genes was a novel locus underlying lean mass variation.
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- 2017
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41. Aberrant Epicardial Adipose Tissue Extracellular Matrix Remodeling in Patients with Severe Ischemic Cardiomyopathy: Insight from Comparative Quantitative Proteomics
- Author
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Bo Huo, Xin Yi, Rui Li, Ding-Sheng Jiang, Xiang Wei, Ligang Liu, Qing Yang, Jing Fang, Yun-Shu Su, Min Hu, Xue-Hai Zhu, Cai Cheng, and Hao-Long Zeng
- Subjects
Male ,Proteomics ,0301 basic medicine ,Pathology ,medicine.medical_specialty ,Proteome ,Quantitative proteomics ,Myocardial Ischemia ,Gene Expression ,Adipose tissue ,030204 cardiovascular system & hematology ,Biology ,Article ,Extracellular matrix ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Humans ,Gene Regulatory Networks ,Protein Interaction Maps ,Multidisciplinary ,Ischemic cardiomyopathy ,Hypertrophic cardiomyopathy ,Middle Aged ,medicine.disease ,Extracellular Matrix ,Cell biology ,030104 developmental biology ,Adipose Tissue ,Female ,Collagen ,Signal transduction ,Cardiomyopathies ,Pericardium ,Signal Transduction ,Extracellular matrix organization - Abstract
There is ample evidence indicating that epicardial adipose tissue (EAT) volume and thickness is positively associated with coronary artery disease (CAD). However, the exact pathological changes in the human EAT after myocardial ischemia remains largely unclear. In the current study, we applied a comparative quantitative proteomics to elucidate the altered biological processes in the EAT of ischemic cardiomyopathy (ICM) patients. A total of 1649 proteins were successfully quantified in our study, among which 165 proteins were significantly changed (ratio 1.2 fold and p
- Published
- 2017
42. Noninvasive prenatal diagnosis of 21-Hydroxylase deficiency using target capture sequencing of maternal plasma DNA
- Author
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Wei Wang, Dingyuan Ma, Yuan Yuan, Zhengfeng Xu, Tao Jiang, Fengyu Guo, Jian Cheng, Yaoshen Wang, Chunyu Luo, Asan, Jingjing Zhang, Jian Wang, Chao Chen, Yun Sun, Xin Yi, Huanming Yang, and Ping Hu
- Subjects
0301 basic medicine ,Genotype ,Sequence analysis ,Science ,Cost-Benefit Analysis ,Prenatal diagnosis ,Reproductive health and childbirth ,030105 genetics & heredity ,Biology ,Deep sequencing ,Article ,Andrology ,03 medical and health sciences ,Congenital ,Plasma ,Rare Diseases ,Prenatal Diagnosis ,medicine ,Genetics ,Humans ,Congenital adrenal hyperplasia ,Adrenal Hyperplasia ,Pediatric ,Fetus ,screening and diagnosis ,Multidisciplinary ,Adrenal Hyperplasia, Congenital ,Haplotype ,DNA ,Sequence Analysis, DNA ,Perinatal Period - Conditions Originating in Perinatal Period ,medicine.disease ,4.1 Discovery and preclinical testing of markers and technologies ,genomic DNA ,Detection ,030104 developmental biology ,Molecular Diagnostic Techniques ,Medicine ,Steroid 21-Hydroxylase ,Sequence Analysis - Abstract
Here, we aimed to validate a noninvasive method using capture sequencing for prenatal diagnosis of congenital adrenal hyperplasia due to 21-Hydroxylase deficiency (21-OHD). Noninvasive prenatal diagnosis (NIPD) of 21-OHD was based on 14 plasma samples collected from 12 families, including four plasma sample collected during the first trimester. Targeted capture sequencing was performed using genomic DNA from the parents and child trios to determine the pathogenic and wild-type alleles associated with the haplotypes. Maternal plasma DNA was also sequenced to determine the fetal inheritance of the allele using hidden Markov model-based haplotype linkage analysis. The effect of fetal DNA fraction and sequencing depth on the accuracy of NIPD was investigated. The lower limit of fetal DNA fraction was 2% and the threshold mean sequence depth was 38, suggesting potential advantage if used in early gestation. The CYP21A2 genotype of the fetus was accurately determined in all the 14 plasma samples as early as day 1 and 8 weeks of gestation. Results suggest the accuracy and feasibility of NIPD of 21-OHD using a small target capture region with a low threshold for fetal DNA fraction and sequence depth. Our method is cost-effective and suggests diagnostic applications in clinical practice.
- Published
- 2017
43. Bilirubin augments Ca
- Author
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Min, Liang, Xin-Lu, Yin, Hai-Bo, Shi, Chun-Yan, Li, Xin-Yi, Li, Ning-Ying, Song, Hao-Song, Shi, Yi, Zhao, Lu-Yang, Wang, and Shan-Kai, Yin
- Subjects
Neurons ,Patch-Clamp Techniques ,Animals, Newborn ,Animals ,Bilirubin ,Calcium ,Calcium Channels ,Cells, Cultured ,Article ,Rats - Abstract
Neonatal brain is particularly vulnerable to pathological levels of bilirubin which elevates and overloads intracellular Ca2+, leading to neurotoxicity. However, how voltage-gated calcium channels (VGCCs) are functionally involved in excess calcium influx remains unknown. By performing voltage-clamp recordings from bushy cells in the ventral cochlear nucleus (VCN) in postnatal rat pups (P4-17), we found the total calcium current density was more than doubled over P4-17, but the relative weight of VGCC subtypes changed dramatically, being relatively equal among T, L, N, P/Q and R-type at P4-6 to predominantly L, N, R over T and P/Q at P15-17. Surprisingly, acute administration of bilirubin augmented the VGCC currents specifically mediated by high voltage-activated (HVA) P/Q-type calcium currents. This augment was attenuated by intracellular loading of Ca2+ buffer EGTA or calmodulin inhibitory peptide. Our findings indicate that acute exposure to bilirubin increases VGCC currents, primarily by targeting P/Q-type calcium channels via Ca2+ and calmodulin dependent mechanisms to overwhelm neurons with excessive Ca2+. Since P/Q-subtype calcium channels are more prominent in neonatal neurons (e.g. P4-6) than later stages, we suggest this subtype-specific enhancement of P/Q-type Ca2+ currents likely contributes to the early neuronal vulnerability to hyperbilirubinemia in auditory and other brain regions.
- Published
- 2016
44. Co-expression network analyses identify functional modules associated with development and stress response in Gossypium arboreum
- Author
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Wenying Xu, Qianhua Wang, Dongxia Yao, Qi You, Fuguang Li, Kang Zhang, Liwei Zhang, Xinhua Zhao, Xin Yi, Yi Ling, Zhen Su, and Xueyan Zhang
- Subjects
0301 basic medicine ,Salinity ,Arabidopsis ,Gene regulatory network ,Computational biology ,Genes, Plant ,Gossypium ,Plant Roots ,Article ,03 medical and health sciences ,Gene Expression Regulation, Plant ,Stress, Physiological ,Botany ,Transcriptional regulation ,Gene Regulatory Networks ,Cotton Fiber ,Gene ,Comparative genomics ,Multidisciplinary ,biology ,Sequence Analysis, RNA ,Gene Expression Profiling ,Salt Tolerance ,Plants, Genetically Modified ,biology.organism_classification ,Gene expression profiling ,Phenotype ,030104 developmental biology ,Organ Specificity ,Function (biology) ,Network analysis - Abstract
Cotton is an economically important crop, essential for the agriculture and textile industries. Through integrating transcriptomic data, we discovered that multi-dimensional co-expression network analysis was powerful for predicting cotton gene functions and functional modules. Here, the recently available transcriptomic data on Gossypium arboreum, including data on multiple growth stages of tissues and stress treatment samples were applied to construct a co-expression network exploring multi-dimensional expression (development and stress) through multi-layered approaches. Based on differential gene expression and network analysis, a fibre development regulatory module of the gene GaKNL1 was found to regulate the second cell wall through repressing the activity of REVOLUTA, and a tissue-selective module of GaJAZ1a was examined in response to water stress. Moreover, comparative genomics analysis of the JAZ1-related regulatory module revealed high conservation across plant species. In addition, 1155 functional modules were identified through integrating the co-expression network, module classification and function enrichment tools, which cover functions such as metabolism, stress responses, and transcriptional regulation. In the end, an online platform was built for network analysis (http://structuralbiology.cau.edu.cn/arboreum), which could help to refine the annotation of cotton gene function and establish a data mining system to identify functional genes or modules with important agronomic traits.
- Published
- 2016
45. Assessment of Pulmonary Gas Transport in Rabbits Using Hyperpolarized Xenon-129 Magnetic Resonance Imaging
- Author
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Ruppert, Kai, primary, Hamedani, Hooman, additional, Amzajerdian, Faraz, additional, Xin, Yi, additional, Duncan, Ian F., additional, Profka, Harrilla, additional, Siddiqui, Sarmad, additional, Pourfathi, Mehrdad, additional, Kadlecek, Stephen, additional, and Rizi, Rahim R., additional
- Published
- 2018
- Full Text
- View/download PDF
46. Adipose mesenchymal stem cells from osteoporotic donors preserve functionality and modulate systemic inflammatory microenvironment in osteoporotic cytotherapy
- Author
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Zheng, Chen-Xi, primary, Sui, Bing-Dong, additional, Liu, Nu, additional, Hu, Cheng-Hu, additional, He, Tao, additional, Zhang, Xin-Yi, additional, Zhao, Pan, additional, Chen, Ji, additional, Xuan, Kun, additional, and Jin, Yan, additional
- Published
- 2018
- Full Text
- View/download PDF
47. Lung Metabolism and Inflammation during Mechanical Ventilation; An Imaging Approach
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Pourfathi, Mehrdad, primary, Cereda, Maurizio, additional, Chatterjee, Shampa, additional, Xin, Yi, additional, Kadlecek, Stephen, additional, Duncan, Ian, additional, Hamedani, Hooman, additional, Siddiqui, Sarmad, additional, Profka, Harrilla, additional, Ehrich, Jason, additional, Ruppert, Kai, additional, and Rizi, Rahim R., additional
- Published
- 2018
- Full Text
- View/download PDF
48. Biological corneal inlay for presbyopia derived from small incision lenticule extraction (SMILE)
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Liu, Yu-Chi, primary, Teo, Ericia Pei Wen, additional, Ang, Heng Pei, additional, Seah, Xin Yi, additional, Lwin, Nyein Chan, additional, Yam, Gary Hin Fai, additional, and Mehta, Jodhbir S., additional
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- 2018
- Full Text
- View/download PDF
49. Regulatory Compliant Tissue-Engineered Human Corneal Endothelial Grafts Restore Corneal Function of Rabbits with Bullous Keratopathy
- Author
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Peh, Gary S. L., primary, Ang, Heng-Pei, additional, Lwin, Chan N., additional, Adnan, Khadijah, additional, George, Benjamin L., additional, Seah, Xin-Yi, additional, Lin, Shu-Jun, additional, Bhogal, Maninder, additional, Liu, Yu-Chi, additional, Tan, Donald T., additional, and Mehta, Jodhbir S., additional
- Published
- 2017
- Full Text
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50. Mesenchymal progenitors in osteopenias of diverse pathologies: differential characteristics in the common shift from osteoblastogenesis to adipogenesis
- Author
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Ling Wu, Yan Jin, Xin-Yi Zhang, Meng Li, Xin Fu, Yichen Chen, Chen-Xi Zheng, Bing-Dong Sui, Li Liao, Xinyi Zhao, and Chenghu Hu
- Subjects
0301 basic medicine ,Senescence ,medicine.medical_specialty ,Stromal cell ,Cellular differentiation ,Peroxisome proliferator-activated receptor ,Down-Regulation ,Bone Marrow Cells ,Biology ,Article ,03 medical and health sciences ,Mice ,Bone Density ,Osteogenesis ,Internal medicine ,medicine ,Adipocytes ,Animals ,Cells, Cultured ,chemistry.chemical_classification ,Multidisciplinary ,Adipogenesis ,Osteoblasts ,Mesenchymal stem cell ,Cell Differentiation ,Mesenchymal Stem Cells ,Up-Regulation ,RUNX2 ,Mice, Inbred C57BL ,Bone Diseases, Metabolic ,030104 developmental biology ,Endocrinology ,medicine.anatomical_structure ,chemistry ,Osteoporosis ,Female ,Bone marrow ,Stromal Cells - Abstract
Osteoporosis is caused by pathologic factors such as aging, hormone deficiency or excess, inflammation and systemic diseases like diabetes. Bone marrow stromal cells (BMSCs), the mesenchymal progenitors for both osteoblasts and adipocytes, are modulated by niche signals. In differential pathologic states, the pathological characteristics of BMSCs to osteoporoses and functional differences are unknown. Here, we detected that trabecular bone loss co-existed with increased marrow adiposity in 6 osteoporotic models, respectively induced by natural aging, accelerated senescence (SAMP6), ovariectomy (OVX), type 1 diabetes (T1D), excessive glucocorticoids (GIOP) and orchidectomy (ORX). Of the ex vivo characteristics of BMSCs, the colony-forming efficiency and the proliferation rate in aging, SAMP6, OVX, GIOP and ORX models decreased. The apoptosis and cellular senescence increased except in T1D, with up-regulation of p53 and p16 expression. The osteogenesis declined except in GIOP, with corresponding down-regulation of Runt-related transcription factor 2 (RUNX2) expression. The adipogenesis increased in 6 osteoporotic models, with corresponding up-regulation of Peroxisome proliferator activated receptor gamma (PPARγ) expression. These findings revealed differential characteristics of BMSCs in a common shift from osteoblastogenesis to adipogenesis among different osteoporoses and between sexes and provide theoretical basis for the functional modulation of resident BMSCs in the regenerative therapy for osteoporosis.
- Published
- 2016
- Full Text
- View/download PDF
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