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19 results on '"Wysocka, A."'

2. Serine proteases and metalloproteases are highly increased in irritable bowel syndrome Tunisian patients

Author

Souha Soussou, Amin Jablaoui, Vincent Mariaule, Aicha Kriaa, Houda Boudaya, Magdalena Wysocka, Ali Amouri, Ali Gargouri, Adam Lesner, Emmanuelle Maguin, and Moez Rhimi

Subjects
Medicine, Science
Abstract

Abstract Serine proteases are involved in many biological processes and are associated with irritable bowel syndrome (IBS) pathology. An increase in serine protease activity has been widely reported in IBS patients. While most of the studies focused on host proteases, the contribution of microbial proteases are poorly studied. In the present study, we report the analysis of proteolytic activities in fecal samples from the first Tunisian cohort of IBS-M patients and healthy individuals. We demonstrated, for the first time, that metalloproteases activities were fourfold higher in fecal samples of IBS patients compared to controls. Of interest, the functional characterization of serine protease activities revealed a 50-fold increase in trypsin-like activities and a threefold in both elastase- and cathepsin G-like activities. Remarkably, we also showed a fourfold increase in proteinase 3-like activity in the case of IBS. This study also provides insight into the alteration of gut microbiota and its potential role in proteolytic modulation in IBS. Our results stressed the impact of the disequilibrium of serine proteases, metalloproteases and gut microbiota in IBS and the need of the further characterization of these targets to set out new therapeutic approaches.

Published
2023
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4. Choroidal thickness in children with type 1 diabetes depending on the pubertal status and metabolic parameters analyzed by optical coherence tomography

Author

Wysocka-Mincewicz Marta, Olechowski Andrzej, Baszyńska-Wilk Marta, Byczyńska Aleksandra, and Gołębiewska Joanna

Subjects
Medicine, Science
Abstract

Abstract To assess choroidal thickness (CT) in children with type 1diabetes (T1D) regarding their pubertal status and seek for factors influencing this parameter, using optical coherence tomography. Material and methods: 333 eyes out of 167 children with T1D without symptoms of diabetic retinopathy (mean age 12.81 ± 3.63 years, diabetes duration 4.59 ± 3.71 years) were enrolled. CT in all quadrants was evaluated. The studied population was divided into three groups: prepubertal, pubertal and postpubertal. The multivariate regression model was carried out using all metabolic parameter and then it was built using only the significant ones. Results: Significant differences in CT between males and females, except nasal and superior quadrants were observed. We revealed significant differences in CT between the three independent groups (Chi-square 18.6, p

Published
2021
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5. A unified resource and configurable model of the synapse proteome and its role in disease

Author

Oksana Sorokina, Colin Mclean, Mike D. R. Croning, Katharina F. Heil, Emilia Wysocka, Xin He, David Sterratt, Seth G. N. Grant, Thomas I. Simpson, and J. Douglas Armstrong

Subjects
Medicine, Science
Abstract

Abstract Genes encoding synaptic proteins are highly associated with neuronal disorders many of which show clinical co-morbidity. We integrated 58 published synaptic proteomic datasets that describe over 8000 proteins and combined them with direct protein–protein interactions and functional metadata to build a network resource that reveals the shared and unique protein components that underpin multiple disorders. All the data are provided in a flexible and accessible format to encourage custom use.

Published
2021
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10. Author Correction: A unified resource and configurable model of the synapse proteome and its role in disease

Author

David C. Sterratt, Colin Mclean, J. Douglas Armstrong, T. Ian Simpson, Xin He, Katharina Heil, Oksana Sorokina, Mike D. R. Croning, Seth G. N. Grant, and Emilia Wysocka

Subjects
Neurons, Proteomics, Multidisciplinary, Proteome, Computer science, Science, Disease, Computational biology, Synapse, Resource (project management), Databases, Genetic, Protein Interaction Mapping, Synapses, Medicine, Humans, Author Correction
Abstract

Genes encoding synaptic proteins are highly associated with neuronal disorders many of which show clinical co-morbidity. We integrated 58 published synaptic proteomic datasets that describe over 8000 proteins and combined them with direct protein-protein interactions and functional metadata to build a network resource that reveals the shared and unique protein components that underpin multiple disorders. All the data are provided in a flexible and accessible format to encourage custom use.

Published
2021

12. Continental-scale patterns of hyper-cryptic diversity within the freshwater model taxon Gammarus fossarum (Crustacea, Amphipoda)

Author

Thierry Rigaud, Krešimir Žganec, Rémi Wattier, Mišel Jelić, Christophe Piscart, Michael Danger, Denis Copilaş-Ciocianu, Arnaud Chaumot, Tomasz Mamos, Vincent Felten, Anthony Ollivier, Michał Grabowski, Anna Wysocka, Tomasz Rewicz, Biogéosciences [UMR 6282] [Dijon] (BGS), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), University of Lódź, University of Basel (Unibas), Institute of Ecology of Nature Research Centre, Varaždin City Museum, Riverly (Riverly), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC), Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire Terre et Environnement de Lorraine (OTELo), Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), University of Zadar, University of Guelph, University of Gdańsk (UG), Adour-Garonne water agency (program Dylaq), French Office for Biodiversity (OFB), Ministry of Science and Higher Education, Poland : N N303 579439, University of Lodz, Short-Term Scientific Mission (STSM) of COST Action : 39779 , CA15219, ANR-13-BSV7-0004,MultiStress,Effets combinés de stresseurs anthropiques et du parasitisme sur la variation génétique, le comportement, la physiologie et le rôle fonctionnel d'amphipodes d'eau douce du genre Gammarus(2013), ANR-14-CE21-0006,ProteoGam,Protéomique pour de nouveaux biomarqueurs en écotoxicologie chez les gammares: challenge de la biodiversité et immunoanalyse multiplexée comme outil de diagnostic(2014), Biogéosciences [UMR 6282] (BGS), Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), RiverLy - Fonctionnement des hydrosystèmes (RiverLy), Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire Terre et Environnement de Lorraine (OTELo), Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), and Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0106 biological sciences, 0301 basic medicine, Scale (anatomy), Genetic Linkage, Lineage (evolution), Biodiversity, lcsh:Medicine, Fresh Water, [SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy, Ecotoxicology, 01 natural sciences, lcsh:Science, Phylogeny, Barcode, media_common, Multidisciplinary, Reveals, Genus Niphargus Crustacea, Europe, Phylogeography, Biogeography, Hydrobiology, Molecular ecology, Species delimitation method, Amphipoda, media_common.quotation_subject, Biology, 010603 evolutionary biology, Article, Evolution, Molecular, 03 medical and health sciences, Complex, Animals, DNA Barcoding, Taxonomic, Lineages, Biodiversity assessment, lcsh:R, Genetic Variation, DNA, biology.organism_classification, Crustacean, 030104 developmental biology, Taxon, Evolutionary biology, lcsh:Q, Evolutionary ecology, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Diversity (politics)
Abstract

Traditional morphological diagnoses of taxonomic status remain widely used while an increasing number of studies show that one morphospecies might hide cryptic diversity, i.e. lineages with unexpectedly high molecular divergence. This hidden diversity can reach even tens of lineages, i.e. hyper cryptic diversity. Even well-studied model-organisms may exhibit overlooked cryptic diversity. Such is the case of the freshwater crustacean amphipod model taxon Gammarus fossarum. It is extensively used in both applied and basic types of research, including biodiversity assessments, ecotoxicology and evolutionary ecology. Based on COI barcodes of 4926 individuals from 498 sampling sites in 19 European countries, the present paper shows (1) hyper cryptic diversity, ranging from 84 to 152 Molecular Operational Taxonomic Units, (2) ancient diversification starting already 26 Mya in the Oligocene, and (3) high level of lineage syntopy. Even if hyper cryptic diversity was already documented in G. fossarum, the present study increases its extent fourfold, providing a first continental-scale insight into its geographical distribution and establishes several diversification hotspots, notably south-eastern and central Europe. The challenges of recording hyper cryptic diversity in the future are also discussed.

Published
2020
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14. Correction: Corrigendum: HIPSTR and thousands of lncRNAs are heterogeneously expressed in human embryos, primordial germ cells and stable cell lines

Author

Dinar Yunusov, Joanna Wysocka, Sergio Verjovski-Almeida, Lucas Ferreira daSilva, Toshihiko Ezashi, R. Michael Roberts, and Leticia Anderson

Subjects
0301 basic medicine, 03 medical and health sciences, 030104 developmental biology, Multidisciplinary, Cell culture, Embryo, Germ, Biology, Cell biology
Abstract

Scientific Reports 6: Article number: 32753; published online: 08 September 2016; updated: 16 March 2017 This Article contains errors in Figure 5F–J, where the right-hand y axes ‘Density (lncRNAs)’ are incorrectly given as ‘Density (except lncRNAs)’. The correct Figure 5 appears below as Figure 1.

Published
2017
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15. HIPSTR and thousands of lncRNAs are heterogeneously expressed in human embryos, primordial germ cells and stable cell lines

Author

Dinar Yunusov, Joanna Wysocka, Sergio Verjovski-Almeida, Lucas Ferreira daSilva, Toshihiko Ezashi, Leticia Anderson, and R. Michael Roberts

Subjects
0301 basic medicine, Blastomeres, Cellular differentiation, Population, Biology, Article, Cell Line, 03 medical and health sciences, Gene expression, Animals, Humans, RNA, Messenger, education, Promoter Regions, Genetic, Gene, Embryonic Stem Cells, Oligonucleotide Array Sequence Analysis, Genetics, education.field_of_study, Multidisciplinary, HEK 293 cells, LINHAGEM CELULAR, RNA, Gene Expression Regulation, Developmental, Embryonic stem cell, Corrigenda, 030104 developmental biology, Germ Cells, HEK293 Cells, Transcription Factor AP-2, Cell culture, RNA, Long Noncoding
Abstract

Eukaryotic genomes are transcribed into numerous regulatory long non-coding RNAs (lncRNAs). Compared to mRNAs, lncRNAs display higher developmental stage-, tissue-, and cell-subtype-specificity of expression, and are generally less abundant in a population of cells. Despite the progress in single-cell-focused research, the origins of low population-level expression of lncRNAs in homogeneous populations of cells are poorly understood. Here, we identify HIPSTR (Heterogeneously expressed from the Intronic Plus Strand of the TFAP2A-locus RNA), a novel lncRNA gene in the developmentally regulated TFAP2A locus. HIPSTR has evolutionarily conserved expression patterns, its promoter is most active in undifferentiated cells, and depletion of HIPSTR in HEK293 and in pluripotent H1BP cells predominantly affects the genes involved in early organismal development and cell differentiation. Most importantly, we find that HIPSTR is specifically induced and heterogeneously expressed in the 8-cell-stage human embryos during the major wave of embryonic genome activation. We systematically explore the phenomenon of cell-to-cell variation of gene expression and link it to low population-level expression of lncRNAs, showing that, similar to HIPSTR, the expression of thousands of lncRNAs is more highly heterogeneous than the expression of mRNAs in the individual, otherwise indistinguishable cells of totipotent human embryos, primordial germ cells, and stable cell lines.

Published
2016

16. PEGylated substrates of NSP4 protease: A tool to study protease specificity

Author

Jenne Dieter, Artur Giełdoń, Natalia Gruba, Krzysztof Rolka, Zbigniew Szewczuk, Marcin Sieńczyk, Remigiusz Bąchor, Renata Grzywa, Magdalena Wysocka, Krzysztof Brzozowski, and Adam Lesner

Subjects
0301 basic medicine, Sequence analysis, Peptidomimetic, Stereochemistry, Myeloblastin, In silico, medicine.medical_treatment, Polyethylene glycol, 01 natural sciences, Article, Substrate Specificity, 03 medical and health sciences, chemistry.chemical_compound, medicine, Humans, Fluorescent Dyes, chemistry.chemical_classification, Serine protease, Multidisciplinary, Protease, biology, 010405 organic chemistry, Sequence Analysis, DNA, 0104 chemical sciences, 030104 developmental biology, Enzyme, chemistry, Biochemistry, biology.protein, Peptidomimetics, DNA
Abstract

Herein we present the synthesis of a novel type of peptidomimetics composed of repeating diaminopropionic acid residues modified with structurally diverse heterobifunctional polyethylene glycol chains (abbreviated as DAPEG). Based on the developed compounds, a library of fluorogenic substrates was synthesized. Further library deconvolution towards human neutrophil serine protease 4 (NSP4) yielded highly sensitive and selective internally quenched peptidomimetic substrates. In silico analysis of the obtained peptidomimetics revealed the presence of an interaction network with distant subsites located on the enzyme surface.

Published
2016
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18. PEGylated substrates of NSP4 protease: A tool to study protease specificity

Author

Wysocka, Magdalena, primary, Gruba, Natalia, additional, Grzywa, Renata, additional, Giełdoń, Artur, additional, Bąchor, Remigiusz, additional, Brzozowski, Krzysztof, additional, Sieńczyk, Marcin, additional, Dieter, Jenne, additional, Szewczuk, Zbigniew, additional, Rolka, Krzysztof, additional, and Lesner, Adam, additional

Published
2016
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