1. Investigation of [11C]carfentanil for mu opioid receptor quantification in the rat brain
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Andrew C. Kelleher, Torben D. Pearson, Joseph Ramsey, Wenjing Zhao, Kelly A. O’Conor, Abolghasem Bakhoda, Tyler Stodden, Min Guo, Seth M. Eisenberg, Sarthak V. Shah, Michael L. Freaney, Woochan Kim, Yeona Kang, Dardo Tomasi, Christopher Johnson, Chung-An Fang, Nora D. Volkow, and Sung Won Kim
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Medicine ,Science - Abstract
Abstract [11C]Carfentanil ([11C]CFN) is the only selective carbon-11 labeled radiotracer currently available for positron emission tomography (PET) imaging of mu opioid receptors (MORs). Though used extensively in clinical research, [11C]CFN has not been thoroughly characterized as a tool for preclinical PET imaging. As we were occasionally observing severe vital sign instability in rat [11C]CFN studies, we set out to investigate physiological effects of CFN mass and to explore its influence on MOR quantification. In anesthetized rats (n = 15), significant dose-dependent PCO2 increases and heart rate decreases were observed at a conventional tracer dose range (IV, > 100 ng/kg). Next, we conducted baseline and retest [11C]CFN PET scans over a wide range of molar activities. Baseline [11C]CFN PET studies (n = 27) found that nondisplaceable binding potential (BPND) in the thalamus was positively correlated to CFN injected mass, demonstrating increase of MOR availability at higher injected CFN mass. Consistently, when CFN injected mass was constrained
- Published
- 2024
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